KEGG   ENZYME: 4.1.3.24
Entry
EC 4.1.3.24                 Enzyme                                 
Name
malyl-CoA lyase;
malyl-coenzyme A lyase;
(3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase;
mclA (gene name);
mcl1 (gene name);
(3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase (acetyl-CoA-forming);
L-malyl-CoA lyase
Class
Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases
Sysname
(S)-malyl-CoA glyoxylate-lyase (acetyl-CoA-forming)
Reaction(IUBMB)
(1) (S)-malyl-CoA = acetyl-CoA + glyoxylate [RN:R00473];
(2) (2R,3S)-2-methylmalyl-CoA = propanoyl-CoA + glyoxylate [RN:R00934]
Reaction(KEGG)
R00473 R00934
Substrate
(S)-malyl-CoA [CPD:C04348];
(2R,3S)-2-methylmalyl-CoA [CPD:C06027]
Product
acetyl-CoA [CPD:C00024];
glyoxylate [CPD:C00048];
propanoyl-CoA [CPD:C00100]
Comment
The enzymes from Rhodobacter species catalyse a step in the ethylmalonyl-CoA pathway for acetate assimilation [3,5]. The enzyme from halophilic bacteria participate in the methylaspartate cycle and catalyse the reaction in the direction of malyl-CoA formation [6]. The enzyme from the bacterium Chloroflexus aurantiacus, which participates in the 3-hydroxypropanoate cycle for carbon assimilation, also has the activity of EC 4.1.3.25, (3S)-citramalyl-CoA lyase [2,4].
History
EC 4.1.3.24 created 1972, modified 2014
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec00660  C5-Branched dibasic acid metabolism
ec00680  Methane metabolism
ec00720  Carbon fixation pathways in prokaryotes
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K08691  malyl-CoA/(S)-citramalyl-CoA lyase
K19281  beta-methylmalyl-CoA/(S)-malyl-CoA lyase
K19282  bifunctional (S)-malyl-CoA lyase/thioesterase
Genes
SACZAOT14_23590(mcl1)
LLG44548918_02118(mcl1)
MCAMCA1739
METUGNH96_10430
MMTMetme_2695
MDNJT25_000155
MDHAYM39_08385
MKOMKLM6_1752
METLU737_10260
MPADKEF85_03350
MELLIVG45_03090
MAHMEALZ_3217(mclA1)
MBUREQU24_04630
MPSYCEK71_18035
MMAIsS8_2223
MMOBF6R98_20170
MEINmethR_P0949
MOZMoryE10_14040
MISZMishRS11D_11830
MESLKKZ03_00260
BLEPAL038_08990
THIPN838_00825
TSNW908_06430
RPJN234_12055
CNCCNE_BB1p10140(mcl)
BGPBGL_2c16890
BPLAbpln_2g17560
BPHBphy_5924
BFNOI25_5771 OI25_7353
BCAIK788_0001579
PHSC2L64_21705
PTERC2L65_26290
PTROG5S35_34875
PACSFAZ98_23740
BBROBAU06_24990
BFZBAU07_25025
BOHAKI39_23800
HYNF9K07_00140
CBABSMCB_1586
MPTMpe_A3254
METPC1M51_05895
XYKGT347_12545
NETNeut_0607
NITNAL212_0420
NIINit79A3_0103
NCOAAW31_08285
NURATY38_03755
NSTNstercoris_01831
NLCEBAPG3_012580
METRBSY238_253
NIMW01_13780
NARCNTG6680_0561(mcl)
URUDSM104443_00577(mcl1_1)
FMYHO273_03895
APPCAP2UW1_2508
DEOCAY53_08885
DMLDmul_11170
DAXFDQ92_06220
MLNA9174_19865
MOPMesop_3793
MAMMesau_03584
MAMOA6B35_22110
MESPC1M53_18670
MHUAMCHK_2942
MJREB229_19625
MERDEB233_16970
AAKAA2016_5786
AMIHCO731_05425(mcl1)
PHTBLM14_27150
PLAPlav_3098
PMOBHG718_00970
KMNHW532_00815
RBSRHODOSMS8_01011(mcl)
RHINGR_c26230
SFHSFHH103_02474(cite1)
SFDUSDA257_c22030(mcl1)
SAMESAMCFNEI73_pC0656(mcl)
SINOSS05631_b61290
ESJSJ05684_b48780
EAKEKH55_4315
BRCBCCGELA001_06950
BRADBF49_2901
BOTCIT37_18285
BRQCIT40_04560
BGQX265_31260
BSYMCIT39_02900
BELBE61_53460(citE)
BDGLPJ38_11055
BOPAXW83_07320
BOSBSY19_2901
BVVBHK69_20015
BOIBLM15_06415
BOFFQV39_02445
XAUXaut_3870
XDIEZH22_19265
AZCAZC_1227
LNEFZC33_08695
MEAMex_1p1733(mcl) Mex_1p4295
MDIMETDI2485(mcl) METDI4906
MEXMext_1802 Mext_3917
MCHMchl_2138 Mchl_4285
MPOMpop_1754 Mpop_4390
MZAB2G69_01480 B2G69_16670
MRDMrad2831_1650 Mrad2831_3663
METM446_6315 M446_6559
MNOMnod_7119 Mnod_7178
MORMOC_2043 MOC_4185
METAY590_08615 Y590_19480
MAQUMaq22A_c18385(citE) Maq22A_c19250(mcl)
MPHYMCBMB27_01298 MCBMB27_03145
MEEDA075_20140 DA075_27160
METDC0214_10380 C0214_21595
METXA3862_13170 A3862_22625
METSDK389_15360 DK389_27325
METIDK427_15555 DK427_17480
MMESMMSR116_01155 MMSR116_21315
MTEADK419_14290 DK419_26925
MINDmvi_25720 mvi_28860
MOGMMB17_01150 MMB17_19730
MTADM6G65_09600 M6G65_20420
MSLMsil_1719
MTUNMTUNDRAET4_3402(mcl)
MLGCWB41_04715
RHJHZY79_12425
BBARRHAL1_03825(mcl_2)
CHELAL346_17425
CDQBOQ54_08925
HDNHden_1659 Hden_3153
HDTHYPDE_30183 HYPDE_38943
HMCHYPMC_1941 HYPMC_4395(mcl)
HNIW911_10365 W911_15805
RVARvan_1682
FILBN1229_v1_1240(mcl) BN1229_v1_2672
FIYBN1229_v1_1238(mcl) BN1229_v1_3242
RHZRHPLAN_35810
MCGGL4_3371
METGHT051_02845
BVRBVIR_1803
BLAGBLTE_09690(mclA)
MSCBN69_1171 BN69_2035
MBRYB1812_00060 B1812_17545
MROSEHO51_12185 EHO51_15075
MHEYH2LOC_011805 H2LOC_014625
MPARF7D14_06360 F7D14_09175
MTWCQW49_12235 CQW49_16215
CMETK6K41_15080 K6K41_22170
PLEOOHA_1_00681(mcl) OHA_1_00777(citE)
HDIHDIA_2217(mcl1_1) HDIA_2467(mcl1_2)
BRND1F64_11585
PSINCAK95_23545
NOHG5V57_13010
RBMTEF_05695
PSFPSE_3192
PPHRAPZ00_18415
LAPACP90_14745
LABRCHH27_05755
LABPFJ695_18280
LABTFIU93_20440(mcl2)
LAGGB0E33_26575
SIWGH266_00855
PHRC6569_07730
PSTGE8M01_31685
CCRCC_2999
CCSCCNA_03094
CAKCaul_0731
CSECseg_3490
CHQAQ619_14395
CMBCSW64_04970
CFHC1707_10190
CAUFCSW63_06460
BALBM8231_03195
TSVDSM104635_00778(mcl1)
SILSPO1565 SPO3608(mclA)
SITTM1040_3165
RUAD1823_18170 D1823_20490
RUTFIU92_13270(mcl2) FIU92_19865(mcl3)
RCONK3740_00765 K3740_20040
JANJann_0471
RDERD1_1001(citE) RD1_1160(mclA) RD1_4242(mclA)
RLIRLO149_c002070(mcl1) RLO149_c036850(mcl2)
RPONG3256_17055
DSHDshi_3050(citE2)
PGAPGA1_c30490
PGLPGA2_c28380
PGDGal_00369
PHPPhaeoP97_00458
PPICPhaeoP14_02820
PHQD1820_09925
OATOAN307_c02220
OAROA238_c37200
OTMOSB_28710(mcl1)
OCTFTO60_14185
LMDMETH_02340 METH_18390
LEJETW24_03080 ETW24_17910
LAQUR2C4_14570 R2C4_19790
LEVETW23_00250 ETW23_17075
LCAEK3721_02490 K3721_16185
REDroselon_01454 roselon_02260
CIDP73_3958 P73_4243
CMARIMCC12053_1300
CEHCEW89_10920
MALGMALG_03008
CONTQ29_15155
YPACCEW88_13335
YANAYJ57_18165
TPROGa0080559_TMP1541
PABYGa0080574_TMP2641
SUAMBOO69_01375
SULZC1J03_02230
SULIC1J05_00980
SULDB5M07_15575
SPOTG6548_03345
SMEDJNX03_11790
SINLDSM14862_03056(mcl1)
SPSESULPSESMR1_03496(mcl1)
TOMBWR18_14535
RMMROSMUCSMR3_02149(mcl1) ROSMUCSMR3_03455(mcl1)
ROKRAK1035_1037 RAK1035_2340
RIDRIdsm_02779(mcl1_1) RIdsm_05011(mcl1_2)
ROMEI983_00710 EI983_08270
ROHFIU89_19925(mcl2)
AHTANTHELSMS3_00280(mcl1)
SAGUCDO87_13335 CDO87_15505
THAACFI11_04070
OCDFHY55_18010
MARUFIU81_14310(mcl3)
ROTFIV09_13785(mcl2)
RMHLVO79_03465
MALUKU6B_08980(mcl1) KU6B_14460 KU6B_40050
TGLHFZ77_14905
TMDKUV46_04605
HATRC74_08495
PALXGQA70_15345 GQA70_23220
LSALKBK07_02450
RSPRSP_1771
RSHRsph17029_0417
RSQRsph17025_2481
RSKRSKD131_0071
CAZTLV780_11350
RCPRCAP_rcc03164(citE2)
RHPLPB142_02980
RBLB6K69_09750
PDEPden_0799
PAMIJCM7686_2763 JCM7686_3168
PYEA6J80_07835
PCONB0A89_03920
PZHCX676_04845 CX676_15565
PAROCUV01_10870
PAMNJCM7685_2683 pAMV1p0135(mcl) pAMV3p0611(mcal)
PMUTDPM13_05940
PARREOJ32_05825
PAAKFIU66_06855
PKDF8A10_08855
PPANESD82_17880
PLIAE4191_12750
PSANHGN31_05715
PMAUCP157_02398(mcl1)
PTPRCA23_c05980(mclA)
RSUNHU_02809
RHMB5V46_11900
RHCRGUI_3145
RMBK529_018960
DAAAKL17_3598
DONBSK21_02035
THWBMG03_17130
TECAKL02_016915
LVSLOKVESSMR4R_02838(mcl1)
RBGBG454_02060 BG454_05615
GEHHYN69_16130 HYN69_16155
GFUKM031_08955 KM031_11830
TAWEI545_00080 EI545_09215
SALOEF888_18940
SEDIEBB79_18450 EBB79_24155
BOOE2K80_13755
PSEBEOK75_09010
PPRUFDP22_10475
PAEDG5B38_01665 G5B38_14245
MONG8E03_06220
PAMOBAR1_02110
PSHQF3W81_17090
POZI0K15_04605
PALWPSAL_024060(mcl1)
PGVSL003B_2239
CACTHZ995_04375
RBZB9057_11515
THASC6Y53_06905
HDHG5B40_08975
CMAGCBW24_04620
GCEKYE46_00395
NSMJO391_00315 JO391_12675
ACROK3J57_12105
FAQG5B39_01155
AMAQGO499_03725 GO499_18685
HNEHNE_0078(mclA)
HBAHbal_0778
CBOTATE48_06115
HBCAEM38_14745
RDICMV14_07525
TFVIDJ81_06845
GBEGbCGDNIH1_0055
GBHGbCGDNIH2_0055
GBCGbCGDNIH3_0055
GBSGbCGDNIH4_0055
RAPRHOA_5783(mcl)
SKTIGS68_03250
SMUCJL100_003365
SROEJL101_003125
RRURru_A0217
RRFF11_01090
RPMRSPPHO_00107
MAGamb3977
MGYMGMSRv2__3257
MGRYMSR1_29830(mcl1)
MAGXXM1_1373
MAGNWV31_14965
TMOTMO_0350(citE)
FERFNB15_09270
HTQFRZ44_44960
HADHFRZ61_42590 FRZ61_45950
DEXHWD60_03940
DVNHQ394_01635
TXITH3_02680
THACCSC3H3_02980
TIIDY252_18625
THAIIT893_02795
MAGQMGMAQ_0659
ECOGFIV45_00610
SNEANBZ79_03900
SALUDC74_4078
SALLSAZ_21750
STREGZL_04819
SLDT261_4311
SLCSL103_17980
SNRSNOUR_21930
SGVB1H19_20540
STROSTRMOE7_00865
SPLACP981_36035
SDWK7C20_34930
SAUHSU9_032330
SANUK7396_01540
SINEKI385_01365
SCTSCAT_4364
SCYSCATT_43520
ABRYNYE86_27165
ICAIntca_2587
ARSADJ73_02415
JTEASJ30_03525
JLIEXU32_11365
JMEEEW87_011295
JAYH7A72_03525
TEHGKE56_09880
PEIH9L10_09525
AUSIPK37_13860
NCANoca_4393
NDKI601_0974(mcl1)
NOYEXE57_13540
NOIFCL41_01330
NOOFE634_19600
NSNEXE58_09730
NBEBack2_15770(citE_1)
NANOG5V58_24335
NAROCFH99_21490
NPIG7071_06375
NAQUENKNEFLB_04247(mcl)
NROK8W59_01590
NOQLN652_11495
NCQK6T13_15930
NPCKUV85_11405
PSIMKR76_23920
AERAERYTH_05645
AEZC3E78_04975
AEBC6I20_10755
AEFGEV26_04810
FRAFrancci3_2805
FSYFsymDg_0509
AMDAMED_0079(citE)
AMNRAM_00395
AMMAMES_0076(citE)
AMZB737_0077(citE)
AOIAORI_0068(mcl)
AJAAJAP_00370
AMQAMETH_0090(citE)
AMYCCU254_39710
AMYBBKN51_34375
AABA4R43_28985
AMYYYIM_00345(mcl2)
AORISD37_41460
AACDLWP59_00255
APRTMUY14_36265
AROONQK81_25995
KALKALB_2473
KPHYAOZ06_42935
ALOCRK61727
PMADBAY61_00315
ACTUActkin_00833(mcl1)
EKEEK0264_04565
BSOLFSW04_11520
CWOCwoe_4213
PARNNBH00_07200
SBAEDSM104329_01824(mcl) DSM104329_03745(mcl1)
AYMYM304_14270
ATQGH723_12970
AQZKSP35_02830
IAMHC251_13125
EUZDVS28_a0494
RRSRoseRS_4173
RCARcas_0912
CAUCaur_0174
CHLChy400_0187
CAGCagg_1328
TROtrd_0455
STISthe_2064
GGRHKW67_06650
HDLHALDL1_15310
HHBHhub_3380(aceB)
HNOLT974_13710
HLULT972_01030
HSUHLASF_2007(aceB)
HSFHLASA_1993(aceB)
SALRFQU85_05715
HAADMW046_18245
HALXM0R89_18090 M0R89_21245
SALIL593_04660
SAIMK0C01_12450
HMArrnAC0690(citE1) rrnAC1965(aceB)
HHIHAH_1341(citE1) HAH_2476(aceB)
HHNHISP_06865 HISP_12605
HABSG26_04910 SG26_14710
HTABVU17_06535 BVU17_12045
HALJG9465_08585
HSINKDQ40_00995 KDQ40_06765
NPHNP_4430A(aceB)
NMONmlp_2357(aceB)
NHOHWV23_13655
NSALHWV07_07395
HMUHmuk_2984
HALZE5139_02195
HALLLC1Hm_2321(mcl)
HAZPGBQ70_02195
HALIBV210_07645
HRRHZS55_19985
HPELHZS54_23485
HDOMUK72_12480
SSAIN0B31_16615
HWAHQ_1720A(aceB)
HWCHqrw_1842(aceB)
HVOHVO_1983(aceB1) HVO_B0200(aceB2)
HMEHFX_2078(aceB)
HGIABY42_09665 ABY42_16600
HALEG3A49_07690 G3A49_12900
HLSKU306_02605
HBOHbor_30530
HLMDV707_13970
SRUBC2R22_21540
HALGHUG10_03375
HALUHUG12_13540
HMPK6T50_01580
HREK6T36_01385
HRMK6T25_15035
HACBHbl1158_08495
HDFAArcSl_1031(aceB)
HTUHtur_1590 Htur_4695
HDABB347_04010
HSALJMJ58_00685 JMJ58_24075
HLOJ0X27_02295
HAKZJ0X25_14760
NMGNmag_3338(mcl) Nmag_3363(aceB)
HXAHalxa_3159
NATNJ7G_3289
NPENatpe_2590
NVRFEJ81_11880
NPLFGF80_10920
NAYHYG81_14690
HALYHYG82_17310
HJTDVR14_02935
NGENatgr_3384
HRUHalru_1548
NOUNatoc_1509 Natoc_4308
HLRHALLA_10835
HLCCHINAEXTREME16075
NAJB1756_06555 B1756_12025
NAGAArcMg_4138
NANAArc1_5073
NBGDV706_12980 DV706_17910
NASGCU68_13725 GCU68_18820
SAWLNGM29_08770
 » show all
Reference
1  [PMID:14285256]
  Authors
TUBOI S, KIKUCHI G.
  Title
ENZYMIC CLEAVAGE OF MALYTL-COENZYME A INTO ACETYL-COENZYME A AND GLYOXYLIC ACID.
  Journal
Biochim Biophys Acta 96:148-53 (1965)
DOI:10.1016/0005-2787(65)90618-0
Reference
2  [PMID:12374834]
  Authors
Herter S, Busch A, Fuchs G
  Title
L-Malyl-coenzyme A lyase/beta-methylmalyl-coenzyme A lyase from Chloroflexus aurantiacus, a bifunctional enzyme involved in autotrophic CO(2) fixation.
  Journal
J Bacteriol 184:5999-6006 (2002)
DOI:10.1128/JB.184.21.5999-6006.2002
  Sequence
[cau:Caur_0174]
Reference
3  [PMID:15687206]
  Authors
Meister M, Saum S, Alber BE, Fuchs G
  Title
L-malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase is involved in acetate assimilation of the isocitrate lyase-negative bacterium Rhodobacter capsulatus.
  Journal
J Bacteriol 187:1415-25 (2005)
DOI:10.1128/JB.187.4.1415-1425.2005
  Sequence
Reference
4  [PMID:17259315]
  Authors
Friedmann S, Alber BE, Fuchs G
  Title
Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus.
  Journal
J Bacteriol 189:2906-14 (2007)
DOI:10.1128/JB.01620-06
Reference
5  [PMID:20047909]
  Authors
Erb TJ, Frerichs-Revermann L, Fuchs G, Alber BE
  Title
The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and (3S)- Malyl-CoA thioesterase.
  Journal
J Bacteriol 192:1249-58 (2010)
DOI:10.1128/JB.01267-09
  Sequence
[rsp:RSP_1771]
Reference
6  [PMID:27920298]
  Authors
Borjian F, Han J, Hou J, Xiang H, Zarzycki J, Berg IA.
  Title
Malate Synthase and beta-Methylmalyl Coenzyme A Lyase Reactions in the Methylaspartate Cycle in Haloarcula hispanica.
  Journal
J Bacteriol 199:e00657-16 (2017)
DOI:10.1128/JB.00657-16
Other DBs
ExplorEnz - The Enzyme Database: 4.1.3.24
IUBMB Enzyme Nomenclature: 4.1.3.24
ExPASy - ENZYME nomenclature database: 4.1.3.24
BRENDA, the Enzyme Database: 4.1.3.24
CAS: 37290-67-8

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