KEGG   ENZYME: 4.1.99.1
Entry
EC 4.1.99.1                 Enzyme                                 
Name
tryptophanase;
L-tryptophanase;
L-tryptophan indole-lyase (deaminating);
TNase
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
Sysname
L-tryptophan indole-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-tryptophan + H2O = indole + pyruvate + NH3 (overall reaction) [RN:R00673];
(1a) L-tryptophan = indole + 2-aminoprop-2-enoate [RN:R12758];
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous) [RN:R11100];
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous) [RN:R11099]
Reaction(KEGG)
Substrate
L-tryptophan [CPD:C00078];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
indole [CPD:C00463];
pyruvate [CPD:C00022];
NH3 [CPD:C00014];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
A pyridoxal-phosphate protein, requiring K+. The enzyme cleaves a carbon-carbon bond, releasing indole and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. Also catalyses 2,3-elimination and beta-replacement reactions of some indole-substituted tryptophan analogues of L-cysteine, L-serine and other 3-substituted amino acids.
History
EC 4.1.99.1 created 1972
Pathway
ec00380  Tryptophan metabolism
ec01100  Metabolic pathways
Orthology
K01667  tryptophanase
Genes
PANPODANSg724
FVRFVEG_14027
MAWMAC_08116
MAJMAA_10321
ELAUCREL1_916
EHIEHI_111610(347.t00005)
EDIEDI_311960
EIVEIN_327340
ECOb3708(tnaA)
ECJJW3686(tnaA)
ECDECDH10B_3895(tnaA)
EBWBWG_3399(tnaA)
ECOKECMDS42_3145(tnaA)
ECEZ5203(tnaA)
ECSECs_4645(tnaA)
ECFECH74115_5139(tnaA)
ETWECSP_4756(tnaA)
ELXCDCO157_4381(tnaA)
EOIECO111_4535(tnaA)
EOJECO26_4874(tnaA)
EOHECO103_4450(tnaA)
ECOOECRM13514_4774(tnaA)
ECOHECRM13516_4558(tnaA)
ESLO3K_25305(tnaA)
ESOO3O_00035(tnaA)
ESMO3M_25225(tnaA)
ECKEC55989_4178(tnaA)
ECGE2348C_4019(tnaA)
EOKG2583_4499(tnaA)
ELRECO55CA74_21605(tnaA)
ELHETEC_3999
ECWEcE24377A_4217(tnaA)
EUNUMNK88_4517(tnaA)
ECPECP_3908
ENAECNA114_3857(tnaA)
ECOSEC958_4181(tnaA)
ECVAPECO1_2752(tnaA)
ECOAAPECO78_22410
ECXEcHS_A3922(tnaA)
ECMEcSMS35_4075(tnaA)
ECYECSE_3994
ECRECIAI1_3887(tnaA)
ECQECED1_4399(tnaA)
EUMECUMN_4239(tnaA)
ECTECIAI39_4312(tnaA)
EOCCE10_4352(tnaA)
EBRECB_03592(tnaA)
EBLECD_03592(tnaA)
EBEB21_03536(tnaA)
EBDECBD_4324
ECIUTI89_C4261(tnaA)
EIHECOK1_4157(tnaA)
ECZECS88_4131(tnaA)
ECCc4631(tnaA)
ELOEC042_4065(tnaA)
ELNNRG857_18470(tnaA)
ESEECSF_3553
ECLEcolC_4286
EKOEKO11_4641
EKFKO11_03575(tnaA)
EABECABU_c41910(tnaA)
EDHEcDH1_4259
EDJECDH1ME8569_3596(tnaA)
ELUUM146_18730(tnaA)
ELWECW_m4007(tnaA)
ELLWFL_19585(tnaA)
ELCi14_4224(tnaA)
ELDi02_4224(tnaA)
ELPP12B_c3843(tnaA)
ELFLF82_2274(tnaA)
ECOLLY180_19205(tnaA)
ECOIECOPMV1_04046(tnaA)
ECOJP423_20580(tnaA)
EFEEFER_4004(tnaA)
EALEAKF1_ch2226c(tnaA)
EMAC1192_16980(tnaA)
ESZFEM44_10115(tnaA)
SFLSF3754(tnaA)
SFXS4017(tnaA)
SFVSFV_3805(tnaA)
SFESFxv_4081(tnaA)
SFNSFy_5348
SFSSFyv_5419
SFTNCTC1_04050(tnaA)
SBCSbBS512_E4214(tnaA)
CCONAFK62_02325(tnaA)
CDMAFK67_02310(tnaA)
CMWAFK63_16310(tnaA)
KOXKOX_09730(tnaA)
KOEA225_0649
KOYJ415_28015(tnaA)
KOMHR38_08450(tnaA)
KOKKONIH1_03450(tnaA)
KOCAB185_32730(tnaA)
KLLBJF97_03990
KLWDA718_25075(tnaA)
KGRJJJ10_24515(tnaA)
RORRORB6_15790(tnaA)
RAODSD31_22580(tnaA)
CKOCKO_02849
CSEDJY391_04810(tnaA)
CAMAF384_20245(tnaA)
CAFAL524_03030(tnaA)
CFARCI104_25255(tnaA)
CIRC2U53_09720(tnaA)
CTELGBC03_04390(tnaA)
CITZE4Z61_17090(tnaA)
CARSE1B03_16555(tnaA)
ESCEntcl_2391
KLEAO703_10280(tnaA) AO703_20280(tnaA)
KGOCEW81_05195(tnaA)
KASKATP_09560(tnaA)
KLUK7B04_24365(tnaA)
LAXAPT61_22105(tnaA)
LEIC2U54_04835(tnaA)
LEHC3F35_11655(tnaA)
LEEDVA44_23740(tnaA)
LERGNG29_23095(tnaA)
LEAGNG26_22435(tnaA)
LPNUKQ929_20540(tnaA)
METYMRY16398_49300(tnaA)
AHNNCTC12129_03675(tnaA)
ASUBNLZ15_22310(tnaA)
YREHEC60_03830(tnaA)
PDZHHA33_24855(tnaA)
PSHISAMEA2665130_0893(tnaA)
YENYE0650(tnaA)
YEPYE105_C0770
YELLC20_04527(tnaA)
YEWCH47_2831(tnaA)
YETCH48_953(tnaA)
YEFFORC2_3446
YEEYE5303_26881(tnaA)
YFRAW19_3989(tnaA)
YINCH53_1040(tnaA)
YKRCH54_1801(tnaA)
YMADA391_18765(tnaA)
YHID5F51_03835(tnaA)
YCAF0T03_03840(tnaA)
YASN0H69_03515(tnaA)
SERASer39006_006065(tnaA)
SERQCWC46_06060(tnaA)
SOFNCTC11214_00995(tnaA)
PCCPCC21_036110
PBRAB5S52_03415
DDDDda3937_01355(tnaA)
DZEDd1591_0587
DDCDd586_3553
DZCW909_17045(tnaA)
DSOA4U42_05600(tnaA)
CEDLH89_15965(tnaA)
DFNCVE23_18965(tnaA)
DDQDDI_3576
DAQDAQ1742_03842(tnaA)
DICDpoa569_000581(tnaA)
DDADd703_0626
PAMPANA_0116(tnaA)
PLFPANA5342_4311(tnaA)
PAJPAJ_3278(tnaA)
PAQPAGR_g4160
PSTWDSJ_22365
PALHB1H58_12595
PLUplu0799(tnaA)
PLUMA4R40_03995(tnaA)
PTTVY86_04110(tnaA)
PAKHB0X70_04445
PLUICE143_04375(tnaA)
PVGCRN77_08810
PHAUPH4a_02250
PROTBTA34_11755
PCOLF1325_13670
PCIBF9282_13770
XBVXBW1_0706(tnaA)
XNEXNC1_0444(tnaA) XNC1_2962(tnaA)
XNMXNC2_0435(tnaA) XNC2_2841(tnaA)
XDOXDD1_0413(tnaA)
XHOA9255_12890 A9255_13910(tnaA)
PSIS70_13785(tnaA)
PSXDR96_507(tnaA)
PSTABGK56_16230
PRGRB151_038050(tnaA)
PALACO695_07390
PHEINCTC12003_03267(tnaA)
PRQCYG50_19680
PRJNCTC6933_03369(tnaA)
PVCG3341_16735
PMAGJI723_16735
MMKMU9_1101
MWIMNY66_07345
ETRETAE_0843(tnaA)
ETDETAF_0789
ETEETEE_2719(tnaA)
ETCETAC_04050(tnaA)
EDWQY76_15975(tnaA)
EDLAAZ33_04480(tnaA)
EHOA9798_03465
HITNTHI0831(tnaA)
HIUHIB_08350
HIZR2866_1769(tnaA)
HIKHifGL_000379(tnaA)
HIAH733_0605
HIHNF38_01615(tnaA)
HICNTHIC486_00286(tnaA)
HIXNTHI723_00998(tnaA)
HHZNCTC10839_00483(tnaA)
GLECJD39_09300(tnaA)
HSOHS_0801(tnaA)
HSMHSM_1270
PMUPM1420(tnaA)
PMVPMCN06_1678(tnaA)
PULNT08PM_1733
PMPPmu_16710(tnaA)
PMULDR93_274(tnaA)
PDAG4362423_01740(tnaA)
PSKYA6B44_03335
PATLKGI96_04110(tnaA)
PCAIK7G93_001769(tnaA)
MVRX781_900
MBOSICJ55_01930(tnaA)
AIOEXH44_05600(tnaA)
RPNENCTC8284_00193(tnaA_1)
RHEYFEE42_08330(tnaA)
RHAEIHV77_11575(tnaA)
OOIA6A10_02035
FCLA4G17_06315
VCHVC_A0161
VCFIR04_00720(tnaA)
VCSMS6_A0141
VCEVch1786_II0954(tnaA)
VCQEN18_04510(tnaA)
VCJVCD_000086
VCIO3Y_14228(tnaA)
VCOVC0395_1117(tnaA)
VCRVC395_A0154(tnaA)
VCMVCM66_A0159(tnaA)
VCLVCLMA_B0143
VCXVAA049_1753(tnaA)
VCZVAB027_874(tnaA)
VVUVV2_0854(tnaA)
VVYVVA1325
VVMVVMO6_04222
VVLVV93_v1c42380(tnaA)
VPAVPA0192
VPBVPBB_A0178
VPKM636_00290(tnaA)
VPFM634_19065(tnaA)
VPHVPUCM_20185
VHAVIBHAR_06709
VCAM892_18930(tnaA)
VAGN646_3629
VEXVEA_000805
VDBAL552_02290(tnaA)
VHRAL538_20685(tnaA)
VNAPN96_15750(tnaA)
VOWA9237_17005(tnaA) A9237_24140
VROBSZ04_05365(tnaA)
VSPVS_1262
VEJVEJY3_23856(tnaA)
VFUvfu_B01026
VNIVIBNI_A0261(tnaA)
VANVAA_01202
LAGN175_11350(tnaA)
VAUVANGNB10_cI0734c(tnaA)
VCYIX92_23840(tnaA) IX92_27170(tnaA)
VCTJV59_24025(tnaA) JV59_25700(tnaA) JV59_30555(tnaA)
VTUIX91_08130(tnaA) IX91_22075(tnaA) IX91_23675(tnaA)
VEUIXK98_05775 IXK98_16740(tnaA) IXK98_24560
VSIMTO69_15865
VFLAL536_07365(tnaA)
VMIAL543_02320(tnaA)
VSCVSVS12_04542 VSVS12_04570
VGABSQ33_21350
VSHBSZ05_15630 BSZ05_23090(tnaA)
VAFD1115_16420
VNLD3H41_21655
VCCFAZ90_06610(tnaA)
VSRVspart_01120(tnaA)
VKABTD91_00275(tnaA)
VZIG5S32_03555(tnaA)
VNVIF132_01700(tnaA)
VGIMID13_09080(tnaA)
VSLLTQ54_15740(tnaA)
VPLSA104470976_03032(tnaA)
VJPNP165_16445
AWDAWOD_I_1599(tnaA)
PPRPBPRA1344 PBPRA2532 PBPRB0382
PGBH744_1c0148
PGHFH974_23495(tnaA) FH974_23590
GHOAL542_02385(tnaA)
PSWLK03_10530(tnaA)
SSESsed_3916
SPSWSps_01497
SPSHFM037_17125
SCYPJYB88_15355
SSEMJYB85_15820
LSDEMK97_00790
MIIBTJ40_09600(tnaA)
MICTFIU95_10885(tnaA)
MICZGL2_33720(tnaA)
GSNYC6258_01928
EEINX720_12365
AHAAHA_1963
AHYAHML_10600(tnaA)
AHDAI20_09355(tnaA)
AHRV428_10890(tnaA)
AHPV429_10900(tnaA)
AHJV469_12505(tnaA)
AHHRY45_10515(tnaA)
AHIVU14_12410(tnaA)
ASAASA_2330(tnaA)
AEOO23A_p1614
AVRB565_2093
AVOAMS64_10185(tnaA)
AMEDB224_5641
ADHCK627_16240(tnaA)
ACAVVI35_20810(tnaA)
AEMCK911_07465(tnaA)
AEAC2U39_21540(tnaA)
ARVC7N77_04550(tnaA)
AESC2U30_14875(tnaA)
AELNCTC12917_04171(tnaA)
AALLI6G90_05710
AJDI6H43_20710
ASAAKXJ75_24025(tnaA)
CHJNCTC10426_01845(tnaA)
ORBIPMB12_10185
CVICV_1163(tnaA)
CVCBKX93_18915(tnaA)
CHRIDK842_13385(tnaA)
CHRBDK843_18840(tnaA)
CHRMFYK34_01430
PSENH8B_1029
AMAHDLM_0375
AQSDKK66_02995(tnaA)
VOGLCH97_08330
CDNBN940_09186
RFRRfer_2475
RACRA876_07355
COFFOZ74_12240
ADNAlide_3129
ADKAlide2_1318
SIMPC6571_17110
MELAC6568_02920
MJELVC68_11595
NOKFAY22_04185
AZAAZKH_4575(tnaA)
DVUDVU_2204(tnaA)
DVLDvul_1032
DVGDeval_2055
AORYAMOR_00300(tnaA)
APAUAMPC_39770(tnaA)
MSDMYSTI_00930
MFBMFUL124B02_35910
RLTRleg2_4875
RPARPA3564
PSFPSE_p0052(tpl)
LABPFJ695_08345
TMOTMO_c0531(tnaA)
AIAAWH56_003570
CTHUHUR95_16640
SRAILN051_04300
TABCIG75_15650
CTCCTC_01509
CTETBN906_01677(tnaA)
CNONT01CX_2071
CLBClo1100_1733
CACECACET_c32610(tnaA)
CEUA7L45_03865
CCAAKQH81_10115
AMTAmet_3003
GFEGferi_20905
CRSFQB35_02970
CRASKVH43_01985
PBIFKXZ80_04540
CLOHMPREF0868_1596
VFAMM35RIKEN_12190(tpl)
ACOLK5I23_08455 K5I23_12190
CSHClosa_3812
LUAD4A81_12835
CASPNQ535_06980
PSORRSJ16_04470
PBQC3V37_06620
STHSTH439(tna-2) STH441(tna-1)
DSYDSY1332 DSY4042
DHDDhaf_1324 Dhaf_2460
DDHDesde_1258 Desde_1942
DORDesor_2544
DAIDesaci_3976
DRMDred_2018
DFGB0537_11105
AACXDEACI_2826
CBARPATL70BA_2536(tnaA)
VGUHYG85_08800
VPYHZI73_01940
ACAEHYG86_02730
THEFE1B22_00910
FMSM1R53_06160
TTETTE1602(TnaA)
TEPTepRe1_2348
TAETepiRe1_2524(tnaA)
TOCToce_0634
NTHNther_2022
HORHore_20900
AFTBBF96_07640
KMEH0A61_01773(tpl)
MHAPJFY71_03950
KPARJL105_06865
CAZOG3A45_06025
ECOUM1R54_08245
PUFUFO1_0888
PFTJBW_04294
MANRMPAN_016640
MBJKQ51_01254(tnaA)
XCLG4Z02_08840
TBMBK010_09925
TBZBK011_09965
TBKHF295_07440
SVLStrvi_6349 Strvi_8511
SRCM271_29125(tnaA)
SMALSMALA_3214
SSOII1A49_19875
SAUOBV401_19575
SSIAA7J05_03675
SFYGFH48_07915
SPHVF9278_05175
SCHACP983_30385
STUIGCM10017668_67990(tnaA)
SDECL3078_01570 L3078_06625
SDURM4V62_42895
KSKKSE_04600(tnaA)
ABRYNYE86_00400
AGMDCE93_01720
SANWG7063_08185
ICAIntca_0141
ARSADJ73_16090
AUSIPK37_10020
PACPPA1068
PAKHMPREF0675_4128
PAVTIA2EST22_05315(tnaA)
PAXTIA2EST36_05285(tnaA)
PAZTIA2EST2_05225(tnaA)
PAWPAZ_c11120(tnaA)
PADTIIST44_09280(tnaA)
PCNTIB1ST10_05480(tnaA)
PACCPAC1_05600(tnaA)
PACHPAGK_1083
PACNTIA1EST1_05245(tnaA)
PRLBCB70_01960
SENSACE_1957
SVISvir_37510
SACCEYD13_20825(tnaA1)
AMQAMETH_4408(tnaA)
AMYCCU254_00135 CU254_12745 CU254_42175
AACDLWP59_01735 LWP59_22600
SSYIEKG83_35075
KALKALB_2889
KPHYAOZ06_19335
LEDBBK82_44630
PMADBAY61_22865
ACTUActkin_02732(tnaA1)
MICHFJK98_01375
AMSAMIS_22590
ACTRAsp14428_20920
ATLAthai_68160(tnaA)
DFUDfulv_46365
DROSDrose_36550
CCAZCOUCH_23195
ABATCFX1CAM_0064(tnaA)
LEKhrd7_02370(tpl)
CAPCLDAP_10980(tnaA)
DFCDFI_10620
TDETDE_0251(tnaA)
TPEDTPE_1510(tnaA)
TPKJO40_03810
TRMJO41_04090
TPHGFUT81_02820
TRZGWP43_01875
TMEDDWB79_04615
SSMSpirs_0133
BHYBHWA1_01919(tnaA)
BHDBHYOB78_06540
BPWWESB_0101(tnaA)
BIPBint_2781(tnaA)
FNUFN1943
FNCHMPREF0946_01787
FNTHMPREF0405_01771
FUSHMPREF0409_01795
FNEFSDG_01509
FHWRN87_03445
FPDCTM68_07980
FVAFV113G1_02940(tnaA)
FGOC4N16_05100
FNFBSQ88_00510
FPEIC4N17_00935
FCII6I83_00005
CSOMMKD34_13485
TLITlie_1330
BTHBT_1492
BTHOBtheta7330_01722(tpl)
BXYBXY_45090
BOABovatus_01573(tnaA1)
BCELBcellWH2_01052(tpl)
BHFC3V43_02205
BISDXK01_019875
BUNBun01g_21110(tnaA)
BEGINE88_00522(tpl)
BSTEK6V31_00025
BFEK6V30_20685
BNOK8P02_11105
BHUMJXR92_009705
PGIPG_1401
PGNPGN_0880
PGTPGTDC60_2059(tnaA)
PAHPoras_0335
PCRENCTC12858_01229(tpl_2)
PCAGNCTC12856_01907(tnaA2)
PENDLA319_05995
PETPEIBARAKI_4317
TOHBCB71_06605
OSPOdosp_1832
BUYD8S85_05690
BVIRI6J59_10205
PITPIN17_A1377
PNIJ5A59_09980
AFDAlfi_0891
ASHAL1_23350
AOKA3BBH6_18500(tnaA)
ADAA5CPEGH6_16250(tnaA)
TTZFHG85_04895
BLQL21SP5_01020(tpl)
BACCBRDCF_p2216(tnaA)
MBASALGA_2691
NSONIASO_14125(tnaA)
SLISlin_0829
LBYLbys_1646
FBAFIC_02450
EAOBD94_0046
EMNM876_17465(tnaA)
EENBBD30_06445
ELBVO54_02560(tpl)
EMGBBD33_03180
EGOBBD34_09600
ELZFCS00_10485
CHRYCEY12_13725
CHRZCO230_11360
CSALNBC122_00880(tpl_1)
CBPEB354_20770
CNPM0D58_11870
CFAELL667_06320
CRHIKB553_14685
CORZMTP08_13930
CSUPMTP09_13795
KHIEG338_02925
KBEJ4771_09670
KFAQ73A0000_11910
KAQL0B70_05075
EZEKI430_12370
FBOJ9309_04020
SHYRLA303_01165
PMOPmob_0309
DTNDTL3_1682(tnaA)
CPOCOPRO5265_1547
BANABARAN1_1003(tnaA)
BIHBIP78_1357
HALVNG_2373G(tnaA)
HSLOE_4331R(tnaA)
HDLHALDL1_16665(tnaA)
HHBHhub_3742(tnaA)
HAGSJT689_11040
HABOJRZ79_09325
HLULT972_14080
HANRLJ422_09605
HJEHacjB3_08945(tnaA)
HALHHTSR_0012
HHSRHSR6_0012(tnaA)
HSUHLASF_0008(tnaA)
HSFHLASA_0008(tnaA)
HALXM0R89_18930
SALIL593_14215(tnaA)
HMArrnAC2439(tnaA)
HHIHAH_2872(tnaA)
HHNHISP_14600(tnaA)
HABSG26_06740
HTABVU17_13970
HSINKDQ40_15100
HDOMUK72_11975
SSAIN0B31_15445
HVOHVO_0009(tnaA)
HMEHFX_0008(tnaA)
HGIABY42_00040
HALEG3A49_00430
HLSKU306_04690
HBOHbor_02170
HALMFCF25_06720
HALGHUG10_12270
HALUHUG12_03805
HTUHtur_0018
HDABB347_14045
HSALJMJ58_09260
HLOJ0X27_06040
HAKZJ0X25_06670
NMGNmag_1937(tnaA)
HXAHalxa_1429
NATNJ7G_0476
NPENatpe_0008
NVRFEJ81_00050
NPLFGF80_00040
NAYHYG81_10535
HJTDVR14_09475
NGENatgr_1589
NOUNatoc_0009
HLRHALLA_19250(tnaA)
HLCCHINAEXTREME04645
NAGAArcMg_0007
NANAArc1_0008
NBGDV706_06955
NASGCU68_06325
SAWLNGM29_02830
APEAPE_1275(tnaA)
PSYTDSAG12_03077(tnaA)
 » show all
Reference
1  [PMID:14017164]
  Authors
BURNS RO, DEMOSS RD.
  Title
Properties of tryptophanase from Escherichia coli.
  Journal
Biochim Biophys Acta 65:233-44 (1962)
DOI:10.1016/0006-3002(62)91042-9
Reference
2  [PMID:14284727]
  Authors
NEWTON WA, MORINO Y, SNELL EE.
  Title
PROPERTIES OF CRYSTALLINE TRYPTOPHANASE.
  Journal
J Biol Chem 240:1211-8 (1965)
Reference
3
  Authors
Cowell, J.L., Maser, K. and DeMoss, R.D.
  Title
Tryptophanase from Aeromonas liquifaciens. Purification, molecular weight and some chemical, catalytic and immunological properties.
  Journal
Biochim Biophys Acta 315:449-463 (1973)
Reference
4  [PMID:236639]
  Authors
Snell EE
  Title
Tryptophanase: structure, catalytic activities, and mechanism of action.
  Journal
Adv Enzymol Relat Areas Mol Biol 42:287-333 (1975)
DOI:10.1002/9780470122877.ch6
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.1
IUBMB Enzyme Nomenclature: 4.1.99.1
ExPASy - ENZYME nomenclature database: 4.1.99.1
BRENDA, the Enzyme Database: 4.1.99.1
CAS: 9024-00-4

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