KEGG   ENZYME: 4.1.99.20
Entry
EC 4.1.99.20                Enzyme                                 
Name
3-amino-4-hydroxybenzoate synthase;
3,4-AHBA synthase;
griH (gene name)
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
Sysname
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate hydro-lyase (cyclizing, 3-amino-4-hydroxybenzoate-forming)
Reaction(IUBMB)
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate = 3-amino-4-hydroxybenzoate + phosphate + 2 H2O [RN:R10469]
Reaction(KEGG)
R10469
Substrate
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate [CPD:C20644]
Product
3-amino-4-hydroxybenzoate [CPD:C12115];
phosphate [CPD:C00009];
H2O [CPD:C00001]
Comment
Requires Mn2+ for maximum activity. The reaction is suggested to take place in several steps. Schiff base formation, double bond migration and dephosphorylation followed by ring opening and closing to form a pyrrolidine ring, and finally dehydration to form the product 3-amino-4-hydroxybenzoate. In the bacterium Streptomyces griseus the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore.
History
EC 4.1.99.20 created 2013, modified 2016
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K17835  3-amino-4-hydroxybenzoic acid synthase
Genes
PAKHB0X70_03100
PLUICE143_03085
POJPtoMrB4_11000
PTWTUM18999_11970
MIIBTJ40_01850
CRZD1345_21920
BURBcep18194_C6863
BCEPAPZ15_37510
BSTLBBJ41_35550
BUZAYM40_17235
PBHAAW51_0199
MANCIV454_17570
SURSTAUR_3233 STAUR_6099
AVMJQX13_12295
CFUSCYFUS_007180
CCROCMC5_017700(aroB)
BWHA9C19_08405
MEKUHUW50_13325 HUW50_25370
PCHIPC41400_02280
PTJJRJ22_24805
CCECcel_2374
SINTK5I26_08865
DDLDesdi_3050
HMOHM1_1788(aroB)
MCHTMCHIJ_41760 MCHIJ_41820
NIEKV110_28195(griH) KV110_32285
RTMG4H71_04125
RGOKYT97_13025
GPOGPOL_c23160
GOMD7316_02314(griH)
GAMGII34_12235
GPDGII33_11545
GOILK459_04840
SGRSGR_4248(griH)
SCBSCAB_69861
SRCM271_02965
SBHSBI_01605
SVESVEN_5125
SVTSVTN_26395
SLDT261_2096
SPRISPRI_1930
STSIA4E84_17115
SLSSLINC_4427 SLINC_5464
SNRSNOUR_07210(griH)
SPLULK06_031740
SGVB1H19_08410
SFKKY5_6733
SLKSLUN_21420
SKACP970_06990
SGALCP966_31005
SPADDVK44_19835
STUIGCM10017668_29370
SFEUIM697_30795
SDWK7C20_14900
SROIIAG44_38355
SMAOCAG99_20770
SLFJEQ17_24460
SDECL3078_11310
STRIC7M71_007295
MIOAOA12_03110
MPHMLP_27050
FRAFrancci3_2069 Francci3_4206
SENSACE_5077
SACGFDZ84_06735 FDZ84_27220
SESPBN6_21370(griH1) BN6_63890(griH2)
SSYIEKG83_36320
KALKALB_2250
KPHYAOZ06_26450
MICBMicB006_2903
MCABHXZ27_13405
MSAGGCM10017556_11890
ACTNL083_5378
PFLAPflav_059800
PSUUPsuf_020550 Psuf_091140
DVCDvina_29175 Dvina_40325
GLYK3N28_00695
MPROBJP34_11735
 » show all
Reference
1  [PMID:17003031]
  Authors
Suzuki H, Ohnishi Y, Furusho Y, Sakuda S, Horinouchi S
  Title
Novel benzene ring biosynthesis from C(3) and C(4) primary metabolites by two enzymes.
  Journal
J Biol Chem 281:36944-51 (2006)
DOI:10.1074/jbc.M608103200
  Sequence
[sgr:SGR_4248]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.20
IUBMB Enzyme Nomenclature: 4.1.99.20
ExPASy - ENZYME nomenclature database: 4.1.99.20
BRENDA, the Enzyme Database: 4.1.99.20

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