KEGG   ENZYME: 4.2.1.107
Entry
EC 4.2.1.107                Enzyme                                 

Name
3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase;
46 kDa hydratase 2;
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase [(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA-forming]
Reaction(IUBMB)
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA = (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O [RN:R04813]
Reaction(KEGG)
R04813;
(other) R04809
Substrate
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA [CPD:C15614]
Product
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA [CPD:C05460];
H2O [CPD:C00001]
Comment
This enzyme forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. The enzyme is involved in the beta-oxidation of the cholesterol side chain in the cholic-acid-biosynthesis pathway.
History
EC 4.2.1.107 created 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec01100  Metabolic pathways
Orthology
K12405  (3R)-3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase / enoyl-CoA hydratase 2
Genes
HSA: 3295(HSD17B4)
PTR: 462019(HSD17B4)
PPS: 100981028(HSD17B4)
GGO: 101129538(HSD17B4)
PON: 100448539(HSD17B4)
NLE: 100590035(HSD17B4)
MCC: 698369(HSD17B4)
MCF: 102123675(HSD17B4)
CSAB: 103244395(HSD17B4)
CATY: 105590649(HSD17B4)
PANU: 101004327(HSD17B4)
RRO: 104654581(HSD17B4)
RBB: 108529825(HSD17B4)
TFN: 117065971(HSD17B4)
PTEH: 111530208(HSD17B4)
CJC: 100390100(HSD17B4)
SBQ: 101036776(HSD17B4)
MMUR: 105872240
MMU: 15488(Hsd17b4)
MCAL: 110284447(Hsd17b4)
MPAH: 110332881(Hsd17b4)
RNO: 79244(Hsd17b4)
MCOC: 116087150(Hsd17b4)
CGE: 103161104(Hsd17b4)
PLEU: 114694768(Hsd17b4)
NGI: 103745113(Hsd17b4)
HGL: 101713520(Hsd17b4)
CCAN: 109674145
OCU: 100346781(HSD17B4)
TUP: 102468828(HSD17B4)
CFA: 474630(HSD17B4)
VVP: 112932403(HSD17B4)
VLG: 121494637(HSD17B4)
AML: 100471749(HSD17B4)
UMR: 103670554(HSD17B4)
UAH: 113261629(HSD17B4)
ORO: 101385731(HSD17B4)
ELK: 111158891
MPUF: 101683185(HSD17B4)
EJU: 114209435(HSD17B4)
MLX: 118016560(HSD17B4)
FCA: 101091840(HSD17B4)
PYU: 121010581(HSD17B4)
PTG: 102968873(HSD17B4)
PPAD: 109278892(HSD17B4)
AJU: 106975089(HSD17B4)
HHV: 120240242(HSD17B4)
BTA: 493643(HSD17B4)
BOM: 102268517(HSD17B4)
BBUB: 102412917(HSD17B4)
CHX: 102172142(HSD17B4)
OAS: 101114519(HSD17B4)
ODA: 120864671(HSD17B4)
CCAD: 122440074(HSD17B4)
SSC: 397574(HSD17B4)
CFR: 102508317(HSD17B4)
CBAI: 105082053(HSD17B4)
CDK: 105104293(HSD17B4)
LVE: 103084787(HSD17B4)
OOR: 101282963(HSD17B4)
DLE: 111183342(HSD17B4)
PCAD: 102996127(HSD17B4)
ECB: 100034046(HSD17B4)
EPZ: 103548315(HSD17B4)
EAI: 106833039(HSD17B4)
MYB: 102240475(HSD17B4)
MYD: 102767159(HSD17B4)
MMYO: 118656167(HSD17B4)
MNA: 107530713(HSD17B4)
HAI: 109374101(HSD17B4)
DRO: 112315031(HSD17B4)
SHON: 118975196(HSD17B4)
AJM: 119051192(HSD17B4)
MMF: 118642891(HSD17B4)
PGIG: 120590862(HSD17B4)
TOD: 119243559(HSD17B4)
LAV: 100659299(HSD17B4)
TMU: 101356123
MDO: 100028098(HSD17B4)
SHR: 100932771(HSD17B4)
PCW: 110202743(HSD17B4)
OAA: 100081754(HSD17B4)
GGA: 395785(HSD17B4)
PCOC: 116234067(HSD17B4)
CJO: 107306670(HSD17B4)
NMEL: 110389447(HSD17B4)
APLA: 101791118(HSD17B4)
ACYG: 106048362(HSD17B4)
TGU: 100218226(HSD17B4)
LSR: 110479177(HSD17B4)
SCAN: 103821465(HSD17B4)
PMOA: 120512524(HSD17B4)
GFR: 102036502(HSD17B4)
FAB: 101807233(HSD17B4)
PHI: 102109547(HSD17B4)
PMAJ: 107216347(HSD17B4)
CCAE: 111941198(HSD17B4)
CCW: 104690497(HSD17B4)
ETL: 114056305(HSD17B4)
FPG: 101917400(HSD17B4)
FCH: 102058406(HSD17B4)
CLV: 102095771(HSD17B4)
EGZ: 104122491(HSD17B4)
NNI: 104014571(HSD17B4)
ACUN: 113489653(HSD17B4)
PADL: 103912347(HSD17B4)
NPD: 112958912(HSD17B4)
ASN: 102371766(HSD17B4)
AMJ: 102571005(HSD17B4)
CPOO: 109323436(HSD17B4)
GGN: 109297318(HSD17B4)
PSS: 102451304(HSD17B4)
CPIC: 101945395(HSD17B4)
TST: 117879364(HSD17B4)
CABI: 116836212(HSD17B4)
ACS: 100558484(hsd17b4)
PVT: 110084344(HSD17B4)
PBI: 103063870(HSD17B4)
PMUR: 107294349(HSD17B4)
TSR: 106543382(HSD17B4)
PGUT: 117674677(HSD17B4)
PMUA: 114606393(HSD17B4)
ZVI: 118076708(HSD17B4)
GJA: 107116225(HSD17B4)
XLA: 444492(hsd17b4.L)
XTR: 613082(hsd17b4)
NPR: 108787696(HSD17B4)
DRE: 393105(hsd17b4)
SANH: 107703629(hsd17b4)
SGH: 107571774(hsd17b4)
IPU: 108265438(hsd17b4)
PHYP: 113529268(hsd17b4)
AMEX: 103046836(hsd17b4)
EEE: 113571049(hsd17b4)
TRU: 101062498(hsd17b4)
LCO: 104920820(hsd17b4)
NCC: 104966339(hsd17b4)
CGOB: 115013548(hsd17b4)
ELY: 117252434(hsd17b4)
PLEP: 121944362(hsd17b4)
SLUC: 116054060(hsd17b4)
ECRA: 117944965(hsd17b4)
PFLV: 114555481(hsd17b4)
GAT: 120830783(hsd17b4)
MSAM: 119891683(hsd17b4)
CUD: 121509730(hsd17b4)
MZE: 101482787(hsd17b4)
ONL: 100706762(hsd17b4)
OAU: 116310500(hsd17b4)
OLA: 101159921(hsd17b4)
OML: 112148954(hsd17b4)
XMA: 102223774(hsd17b4)
XCO: 114154573(hsd17b4)
XHE: 116730397(hsd17b4)
PRET: 103470095(hsd17b4)
CVG: 107086311(hsd17b4)
CTUL: 119798579(hsd17b4)
NFU: 107394149(hsd17b4)
KMR: 108233704(hsd17b4)
ALIM: 106513677(hsd17b4)
AOCE: 111564685(hsd17b4)
POV: 109637138(hsd17b4)
LCF: 108882142(hsd17b4)
SDU: 111230866(hsd17b4)
SLAL: 111645149(hsd17b4)
XGL: 120805559(hsd17b4)
HCQ: 109526033(hsd17b4)
BPEC: 110167024(hsd17b4)
MALB: 109956607(hsd17b4)
SASA: 106585849(hsd17b4)
OTW: 112247069(hsd17b4)
OMY: 110525264(hsd17b4)
SALP: 111974039(hsd17b4)
SNH: 120045172(hsd17b4)
ELS: 105014609(hsd17b4)
SFM: 108938133(hsd17b4)
PKI: 111856620(hsd17b4)
AANG: 118206736(hsd17b4)
LOC: 102697531(hsd17b4)
PSPA: 121302596(hsd17b4) 121316706
LCM: 102356548(HSD17B4)
CMK: 103181119(hsd17b4)
BFO: 118409354
BBEL: 109480804
CIN: 100187421
SCLV: 120338122
SPU: 581580
DME: Dmel_CG3415(Mfe2)
DMN: 108161558
DGR: 6565453
CCAT: 101461807
ACOZ: 120955687
AARA: 120901803
AAG: 5568633
AALB: 109428196
CPII: 120414661
AME: 409986
ACER: 107999187
BBIF: 117204816
NVI: 100123018
CCIN: 107263397
TCA: 663655(17-beta-HD4)
AGB: 108915760
LDC: 111505860
APLN: 108736403
OTU: 111423793
BMOR: 101745499
API: 100167575
DNX: 107163428
AGS: 114120233
RMD: 113555196
HHAL: 106683228
NLU: 111057072
CSEC: 111873091
DMK: 116931070
HAME: 121875772
HAZT: 108681612
EAF: 111696524
DSV: 119440587
RSAN: 119387719
VDE: 111252755
VJA: 111266535
SDM: 118180496
CEL: CELE_E04F6.3(maoc-1) CELE_M03A8.1(dhs-28)
BGT: 106065027
GAE: 121385566
PMAX: 117334218
NVE: 5504006
ATEN: 116294223
DDI: DDB_G0291247(mfeA)
 » show all
Reference
1  [PMID:9371691]
  Authors
Qin YM, Haapalainen AM, Conry D, Cuebas DA, Hiltunen JK, Novikov DK.
  Title
Recombinant 2-enoyl-CoA hydratase derived from rat peroxisomal multifunctional enzyme 2: role of the hydratase reaction in bile acid synthesis.
  Journal
Biochem J 328 ( Pt 2):377-82 (1997)
DOI:10.1042/bj3280377
Reference
2  [PMID:8619845]
  Authors
Xu R, Cuebas DA.
  Title
The reactions catalyzed by the inducible bifunctional enzyme of rat liver peroxisomes cannot lead to the formation of bile acids.
  Journal
Biochem Biophys Res Commun 221:271-8 (1996)
DOI:10.1006/bbrc.1996.0585
Reference
3
  Authors
Kinoshita, T., Miyata, M., Ismail, S.M., Fujimoto, Y., Kakinuma, K., Kokawa, N.I. and Morisaki, M.
  Title
Synthesis and determination of stereochemistry of four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.
  Journal
Chem Pharm Bull 36:134-141 (1988)
Reference
4
  Authors
Fujimoto, Y., Kinoshita, T., Oya, I., Kakinuma, K., Ismail, S.M., Sonoda, Y., Sato, Y. and Morisaki, M.
  Title
Non-stereoselective conversion of the four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.
  Journal
Chem Pharm Bull 36:142-145 (1988)
Reference
5  [PMID:11146090]
  Authors
Kurosawa T, Sato M, Nakano H, Fujiwara M, Murai T, Yoshimura T, Hashimoto T.
  Title
Conjugation reactions catalyzed by bifunctional proteins related to beta-oxidation in bile acid biosynthesis.
  Journal
Steroids 66:107-14 (2001)
DOI:10.1016/S0039-128X(00)00217-8
Reference
6  [PMID:12543708]
  Authors
Russell DW
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu Rev Biochem 72:137-74 (2003)
DOI:10.1146/annurev.biochem.72.121801.161712
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.107
IUBMB Enzyme Nomenclature: 4.2.1.107
ExPASy - ENZYME nomenclature database: 4.2.1.107
BRENDA, the Enzyme Database: 4.2.1.107
CAS: 152787-68-3

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