KEGG   ENZYME: 4.2.1.114
Entry
EC 4.2.1.114                Enzyme                                 
Name
methanogen homoaconitase;
methanogen HACN
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(R)-2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate-forming]
Reaction(IUBMB)
(R)-2-hydroxybutane-1,2,4-tricarboxylate = (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate (overall reaction) [RN:R09720];
(1a) (R)-2-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O [RN:R03444];
(1b) (Z)-but-1-ene-1,2,4-tricarboxylate + H2O = (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [RN:R04371]
Reaction(KEGG)
Substrate
(R)-2-hydroxybutane-1,2,4-tricarboxylate [CPD:C01251];
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:C04002];
H2O [CPD:C00001]
Product
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [CPD:C05662];
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:C04002];
H2O [CPD:C00001]
Comment
This enzyme catalyses several reactions in the pathway of coenzyme-B biosynthesis in methanogenic archaea. Requires a [4Fe-4S] cluster for activity. In contrast to EC 4.2.1.36, homoaconitate hydratase, this enzyme can catalyse both the dehydration of (R)-homocitrate to form cis-homoaconitate and the subsequent hydration reaction that forms homoisocitrate. In addition to cis-homoaconitate, the enzyme can also catalyse the hydration of the physiological substrates dihomoaconitate and trihomoaconitate as well as the non-physiological substrate tetrahomoaconitate. cis-Aconitate and threo-DL-isocitrate cannot act as substrates, and (S)-homocitrate and trans-homoaconitate act as inhibitors of the enzyme.
History
EC 4.2.1.114 created 2009
Pathway
ec00300  Lysine biosynthesis
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K16792  methanogen homoaconitase large subunit
K16793  methanogen homoaconitase small subunit
Genes
PASGKSS96_22110
PEWKZJ38_04415
BOHAKI39_19115
MMAXLCQ47_06430
SSOII1A49_21970
AACDLWP59_12505
RRSRoseRS_1053 RoseRS_1056
RCARcas_3472 Rcas_3474
CAUCaur_0329 Caur_0330
CHLChy400_0354 Chy400_0355
CAGCagg_3601 Cagg_3602
HAUHaur_3302 Haur_3303
LEKhrd7_27860(leuC_2) hrd7_27870(leuD_2)
PMETG4Y79_18425
DRADR_1610 DR_1614(leuD)
DGEDgeo_1154 Dgeo_1156
DDRDeide_13460 Deide_13470
DMRDeima_1353 Deima_1355
DGODGo_CA1306 DGo_CA1308
DPDDeipe_2762 Deipe_2764
DSWQR90_07810 QR90_09055
DCHSY84_02050 SY84_02060
DABAUC44_07725
DPUSU48_08705 SU48_08715
DEZDKM44_08505 DKM44_08520
DWUDVJ83_05635 DVJ83_05640
DFCDFI_07070 DFI_07080
DEINDAAJ005_09955 DAAJ005_11930
DGADEGR_14160(leuC1) DEGR_14180(leuD1)
DPHEHF33_03450 EHF33_05185
DRBN0D28_07410 N0D28_11260
TRATrad_1389 Trad_1390
TTHTT_C1546 TT_C1547
TTJTTHA1910(TTHA1910) TTHA1911(TTHA1911)
TTSThthe16_1910 Ththe16_1911
TTLTtJL18_1992 TtJL18_1993
TSCTSC_c01880(hacA) TSC_c01890(hacB)
THCTCCBUS3UF1_21060 TCCBUS3UF1_21070
TOSTheos_1936 Theos_1937
TAQTO73_0094 TO73_0095
TPARAV541_01240 AV541_01245
TBCA0O31_00873 A0O31_00874
TANTKNN15_05110 KNN15_05115
MRBMrub_2727 Mrub_2728
MREK649_08895 K649_08900
MSVMesil_0441 Mesil_0442
MTAIMtai_v1c23260 Mtai_v1c23270
OPROcepr_1780 Ocepr_1781
MHDMarky_0671 Marky_0672
SCORJ3U87_19130 J3U87_19135
MJAMJ_1003 MJ_1271
MFEMefer_0048 Mefer_1231
MVUMetvu_0677 Metvu_0864 Metvu_1303
MFSMFS40622_0030 MFS40622_0660 MFS40622_1618
MIFMetin_0516 Metin_1437
MJHJH146_0454 JH146_0954
MESGMLAUSG7_0025(hacB) MLAUSG7_0432(hacA)
MESAMLASG1_0006(hacA) MLASG1_1205(hacB)
MIGMetig_0050 Metig_0807 Metig_1570
MMPMMP0381 MMP1480
MMQMmarC5_0098 MmarC5_1257
MMXMmarC6_0529 MmarC6_1194
MMZMmarC7_0724 MmarC7_1379
MMDGYY_08235
MMAKMMKA1_04000(leuD) MMKA1_17030(leuD)
MMAOMMOS7_03940(leuD) MMOS7_15860(leuD)
MMADMMJJ_06580(dmdB_1) MMJJ_13590(dmdA_1)
MAEMaeo_0311 Maeo_0652
MVNMevan_0789 Mevan_1368
MVOMvol_1623 Mvol_1671
MOKMetok_0062 Metok_0567
METFCFE53_04745 CFE53_05095
MTHMTH_1631 MTH_829
MMGMTBMA_c02160(leuC1) MTBMA_c12270(leuD1)
MWOMWSIV6_0177(hacA) MWSIV6_1206(hacB)
MTEEMTTB_05450 MTTB_13810
METCMTCT_0743 MTCT_1490
METEtca_00796(DmdB_1) tca_01581(dmdA_2)
METZMETMT2_0903 METMT2_1470
METKFVF72_03470 FVF72_07395
MTHMFZP57_00775 FZP57_05725
METJFZP68_00480 FZP68_05785
MSTMsp_0374(leuD2) Msp_1100(leuC2)
METBAW729_05200 AW729_10315
MRUmru_0384(aksD) mru_1689(aksE)
MSIMsm_0723 Msm_0847
MEBAbm4_0180(aksD) Abm4_0514(aksE)
MMILsm9_1773(aksE) sm9_2161(aksD)
MEYETL18_01065 TL18_07930
MOLYLM1_0055 YLM1_1157
MAROMarbSA_13800 MarbSA_18800
METVK4897_04160 K4897_07245
MELMetbo_1944 Metbo_2070
MEWMSWAN_0570 MSWAN_0676
METHMBMB1_0289(leuC2) MBMB1_0696(leuD1)
MFCBRM9_1499(aksE) BRM9_1986(aksD)
MFIDSM1535_0178(leuC2) DSM1535_2193(leuD1)
MCUBMCBB_0567(hacA) MCBB_0707(hacB)
MSUBBK009_01700 BK009_11610
METNBK008_07185 BK008_09225
METTCIT01_01955 CIT01_06840
METOCIT02_03290 CIT02_05440
MEMEHYG87_03480 HYG87_07560
MFVMfer_0205 Mfer_0965
MKAMK1206(leuD_2) MK1208(leuC_2)
MBAMbar_A0244 Mbar_A1961 Mbar_A2270
MBYMSBRM_0704 MSBRM_1355 MSBRM_2710
MBWMSBRW_0751 MSBRW_2476 MSBRW_2776
MBARMSBR2_1054 MSBR2_1672 MSBR2_2666
MBAKMSBR3_1018 MSBR3_1673 MSBR3_2760
MACMA_1223(leuC) MA_3085(leuC) MA_3751(leuD)
MMAMM_0409 MM_0645
MMAZMmTuc01_0417 MmTuc01_0660
MMJMSMAS_0664 MSMAS_0894
MMACMSMAC_0666 MSMAC_0883
MVCMSVAZ_0690 MSVAZ_1170 MSVAZ_2221
MEKMSKOL_0668 MSKOL_1122 MSKOL_2220
MLSMSLAZ_2100 MSLAZ_2434
METMMSMTP_0731 MSMTP_2281
MEFMSWH1_0826 MSWH1_0957
MEQMSWHS_0885 MSWHS_1027
MSJMSSAC_2956 MSSAC_3368 MSSAC_3559
MSZMSSIH_2594 MSSIH_2894 MSSIH_3101
MSWMSSIT_2634 MSSIT_2938 MSSIT_3158
MTHRMSTHT_2374 MSTHT_2523
MTHEMSTHC_0762 MSTHC_0906
MHORMSHOH_0786 MSHOH_1131
MFZAOB57_002735 AOB57_003550
MBUMbur_1578 Mbur_2263(hacA)
MMETMCMEM_0412 MCMEM_2089
MELOJ7W08_00085 J7W08_09410
MMHMmah_0127 Mmah_0418
MHAZBHR79_01390 BHR79_02815
MEVMetev_1312 Metev_1371
MZHMzhil_0725 Mzhil_1660
MPYMpsy_2354 Mpsy_2821
MZIHWN40_08640 HWN40_10575
MHZMetho_0481 Metho_0694
MTPMthe_0788 Mthe_0853
MCJMCON_0444(leuC1) MCON_1922(leuD1)
MHIMhar_1525 Mhar_2395
MHUMhun_1799 Mhun_1800
MRTJKHC33_05410 KHC33_05415
MLAMlab_0233 Mlab_0234
MEMMemar_0634 Memar_0635
MBGBN140_1716(leuD3) BN140_1717(leuC3)
MEMAMMAB1_2209(leuC)
MCHKMchiMG62_07640 MchiMG62_07650
MSUMOH143_09890 OH143_09895
MPIMpet_2489 Mpet_2490
MENDL6E24_09225
MBNMboo_0599 Mboo_0600
MFOMetfor_2591 Metfor_2592
MPLMpal_2143 Mpal_2144
MPDMCP_1961 MCP_1962
MEZMtc_1558(leuD-2) Mtc_1559(leuC-2)
RCIRCIX2645(leuD-2) RCIX2646(leuC-2)
SSAIN0B31_20520
CDIVCPM_1676
MEUZKRP56_01645 KRP56_01650 KRP56_05135
 » show all
Reference
1  [PMID:18765671]
  Authors
Drevland RM, Jia Y, Palmer DR, Graham DE
  Title
Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis.
  Journal
J Biol Chem 283:28888-96 (2008)
DOI:10.1074/jbc.M802159200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.114
IUBMB Enzyme Nomenclature: 4.2.1.114
ExPASy - ENZYME nomenclature database: 4.2.1.114
BRENDA, the Enzyme Database: 4.2.1.114

DBGET integrated database retrieval system