KEGG   ENZYME: 4.2.1.119
Entry
EC 4.2.1.119                Enzyme                                 
Name
enoyl-CoA hydratase 2;
2-enoyl-CoA hydratase 2;
AtECH2;
ECH2;
MaoC;
MFE-2;
PhaJAc;
D-3-hydroxyacyl-CoA hydro-lyase;
D-specific 2-trans-enoyl-CoA hydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(3R)-3-hydroxyacyl-CoA hydro-lyase
Reaction(IUBMB)
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H2O [RN:R09698]
Reaction(KEGG)
R09698 > R07935 R07951
Substrate
(3R)-3-hydroxyacyl-CoA [CPD:C01086]
Product
(2E)-2-enoyl-CoA [CPD:C00658];
H2O [CPD:C00001]
Comment
This enzyme catalyses a hydration step in peroxisomal beta-oxidation. The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units [1]. The enzymes from Aeromonas caviae [4] and Arabidopsis thaliana [5] are monofunctional enzymes. 2-Enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2 [3].
History
EC 4.2.1.119 created 2009
Pathway
ec01040  Biosynthesis of unsaturated fatty acids
ec01100  Metabolic pathways
Orthology
K12405  (3R)-3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase / enoyl-CoA hydratase 2
K19658  peroxisomal enoyl-CoA hydratase 2
K19659  enoyl-CoA hydratase
Genes
HSA3295(HSD17B4)
PTR462019(HSD17B4)
PPS100981028(HSD17B4)
GGO101129538(HSD17B4)
PON100448539(HSD17B4)
NLE100590035(HSD17B4)
MCC698369(HSD17B4)
MCF102123675(HSD17B4)
MTHB126957048
CSAB103244395(HSD17B4)
CATY105590649(HSD17B4)
PANU101004327(HSD17B4)
TGE112625827(HSD17B4)
RRO104654581(HSD17B4)
RBB108529825(HSD17B4)
TFN117065971(HSD17B4)
PTEH111530208(HSD17B4)
CJC100390100(HSD17B4)
SBQ101036776(HSD17B4)
CSYR103262046
MMUR105872240
LCAT123648404(HSD17B4)
OGA100966879(HSD17B4)
MMU15488(Hsd17b4)
MCAL110284447(Hsd17b4)
MPAH110332881(Hsd17b4)
RNO79244(Hsd17b4)
MCOC116087150(Hsd17b4)
CGE103161104(Hsd17b4)
MAUA101827399(Hsd17b4)
PLEU114694768(Hsd17b4)
MORG121441957 121453615(Hsd17b4)
MFOT126505968
AAMP119815878(Hsd17b4)
NGI103745113(Hsd17b4)
HGL101713520(Hsd17b4)
CPOC100379226(Hsd17b4)
CCAN109674145
DORD105985471(Hsd17b4)
DSP122101973(Hsd17b4)
NCAR124986751
OCU100346781(HSD17B4)
OPI101530287(HSD17B4)
TUP102468828(HSD17B4)
CFA474630(HSD17B4)
CLUD112659672(HSD17B4)
VVP112932403(HSD17B4)
VLG121494637(HSD17B4)
AML100471749(HSD17B4)
UMR103670554(HSD17B4)
UAH113261629(HSD17B4)
UAR123790430(HSD17B4)
ELK111158891
LLV125100000
MPUF101683185(HSD17B4)
ORO101385731(HSD17B4)
EJU114209435(HSD17B4)
ZCA113928790(HSD17B4)
MLX118016560(HSD17B4)
NSU110590913(HSD17B4)
FCA101091840(HSD17B4)
PYU121010581(HSD17B4)
PBG122486641(HSD17B4)
LRUF124515159
PTG102968873(HSD17B4)
PPAD109278892(HSD17B4)
AJU106975089(HSD17B4)
HHV120240242(HSD17B4)
BTA493643(HSD17B4)
BOM102268517(HSD17B4)
BBUB102412917(HSD17B4)
CHX102172142(HSD17B4)
OAS101114519(HSD17B4)
ODA120864671(HSD17B4)
CCAD122440074(HSD17B4)
SSC397574(HSD17B4)
CFR102508317(HSD17B4)
CBAI105082053(HSD17B4)
CDK105104293(HSD17B4)
VPC102537534(HSD17B4)
LVE103084787(HSD17B4)
OOR101282963(HSD17B4)
DLE111183342(HSD17B4)
PCAD102996127(HSD17B4)
PSIU116751686(HSD17B4)
ECB100034046(HSD17B4)
EPZ103548315(HSD17B4)
EAI106833039(HSD17B4)
MYB102240475(HSD17B4)
MYD102767159(HSD17B4)
MMYO118656167(HSD17B4)
MLF102425700(HSD17B4)
MNA107530713(HSD17B4)
PKL118703106(HSD17B4)
HAI109374101(HSD17B4)
DRO112315031(HSD17B4)
SHON118975196(HSD17B4)
AJM119051192(HSD17B4)
PDIC114494306(HSD17B4)
PHAS123818113(HSD17B4)
MMF118642891(HSD17B4)
RFQ117024890(HSD17B4)
PALE102887000(HSD17B4)
PGIG120590862(HSD17B4)
PVP105290860(HSD17B4)
RAY107511154(HSD17B4)
TOD119243559(HSD17B4)
SARA101542842(HSD17B4)
LAV100659299(HSD17B4)
TMU101356123
DNM101423884(HSD17B4)
MDO100028098(HSD17B4)
GAS123235791(HSD17B4)
SHR100932771(HSD17B4)
PCW110202743(HSD17B4)
OAA100081754(HSD17B4)
GGA395785(HSD17B4)
PCOC116234067(HSD17B4)
CJO107306670(HSD17B4)
NMEL110389447(HSD17B4)
APLA101791118(HSD17B4)
ACYG106048362(HSD17B4)
AFUL116500754(HSD17B4)
TGU100218226(HSD17B4)
LSR110479177(HSD17B4)
SCAN103821465(HSD17B4)
PMOA120512524(HSD17B4)
OTC121332706(HSD17B4)
PRUF121362562(HSD17B4)
GFR102036502(HSD17B4)
FAB101807233(HSD17B4)
PHI102109547(HSD17B4)
PMAJ107216347(HSD17B4)
CCAE111941198(HSD17B4)
CCW104690497(HSD17B4)
CBRC103611629(HSD17B4)
ETL114056305(HSD17B4)
ZAB102062238(HSD17B4)
ACHL103806159(HSD17B4)
FPG101917400(HSD17B4)
FCH102058406(HSD17B4)
CLV102095771(HSD17B4)
EGZ104122491(HSD17B4)
NNI104014571(HSD17B4)
PLET104618021(HSD17B4)
ACUN113489653(HSD17B4)
TALA104363287(HSD17B4)
PADL103912347(HSD17B4)
AFOR103897966(HSD17B4)
ACHC115336530(HSD17B4)
HALD104320961(HSD17B4)
CSTI104557391(HSD17B4)
CMAC104480460(HSD17B4)
MUI104541285(HSD17B4)
FGA104069667(HSD17B4)
LDI104348760(HSD17B4)
OHA104339186(HSD17B4)
NNT104407165(HSD17B4)
DPUB104304758(HSD17B4)
PGUU104458725(HSD17B4)
AVIT104272741(HSD17B4)
CVF104294213(HSD17B4)
AROW112978041(HSD17B4)
NPD112958912(HSD17B4)
TGT104571515(HSD17B4)
DNE112992405
SCAM104150534(HSD17B4)
ASN102371766(HSD17B4)
AMJ102571005(HSD17B4)
CPOO109323436(HSD17B4)
GGN109297318(HSD17B4)
PSS102451304(HSD17B4)
CPIC101945395(HSD17B4)
TST117879364(HSD17B4)
CABI116836212(HSD17B4)
MRV120408024(HSD17B4)
ACS100558484(hsd17b4)
PVT110084344(HSD17B4)
SUND121921111(HSD17B4)
PBI103063870(HSD17B4)
PMUR107294349(HSD17B4)
CTIG120316078(HSD17B4)
TSR106543382(HSD17B4)
PGUT117674677(HSD17B4)
VKO123031365(HSD17B4)
PMUA114606393(HSD17B4)
ZVI118076708(HSD17B4)
GJA107116225(HSD17B4)
STOW125436558(HSD17B4)
XLA444492(hsd17b4.L)
XTR613082(hsd17b4)
NPR108787696(HSD17B4)
RTEM120931986(HSD17B4)
BBUF120992600(HSD17B4)
BGAR122924617(HSD17B4)
DRE393105(hsd17b4)
SANH107703629(hsd17b4)
SGH107571774(hsd17b4)
CAUA113094195 113106903
PPRM120475505(hsd17b4)
MAMB125279588(hsd17b4)
IPU108265438(hsd17b4)
PHYP113529268(hsd17b4)
SMEO124399595(hsd17b4)
TFD113656214(hsd17b4)
AMEX103046836(hsd17b4)
EEE113571049(hsd17b4)
TRU101062498(hsd17b4)
TNGGSTEN00030945G001
LCO104920820(hsd17b4)
NCC104966339(hsd17b4)
CGOB115013548(hsd17b4)
ELY117252434(hsd17b4)
EFO125890843(hsd17b4)
PLEP121944362(hsd17b4)
SLUC116054060(hsd17b4)
ECRA117944965(hsd17b4)
ESP116689590(hsd17b4)
PFLV114555481(hsd17b4)
GAT120830783(hsd17b4)
PPUG119225552(hsd17b4)
MSAM119891683(hsd17b4)
CUD121509730(hsd17b4)
ALAT119019193(hsd17b4)
MZE101482787(hsd17b4)
ONL100706762(hsd17b4)
OAU116310500(hsd17b4)
OLA101159921(hsd17b4)
OML112148954(hsd17b4)
XMA102223774(hsd17b4)
XCO114154573(hsd17b4)
XHE116730397(hsd17b4)
PRET103470095(hsd17b4)
PFOR103141760(hsd17b4)
PLAI106944940(hsd17b4)
PMEI106923064(hsd17b4)
GAF122827419(hsd17b4)
CVG107086311(hsd17b4)
CTUL119798579(hsd17b4)
GMU124877861(hsd17b4)
NFU107394149(hsd17b4)
KMR108233704(hsd17b4)
ALIM106513677(hsd17b4)
NWH119413904(hsd17b4)
AOCE111564685(hsd17b4)
MCEP125012708(hsd17b4)
POV109637138(hsd17b4)
SSEN122769476(hsd17b4)
HHIP117768550(hsd17b4)
HSP118114529(hsd17b4)
LCF108882142
SDU111230866(hsd17b4)
SLAL111645149(hsd17b4)
XGL120805559(hsd17b4)
HCQ109526033(hsd17b4)
BPEC110167024(hsd17b4)
MALB109956607(hsd17b4)
BSPL114861822(hsd17b4)
SASA106585849(hsd17b4)
STRU115164957(hsd17b4)
OTW112247069(hsd17b4)
OMY110525264(hsd17b4)
OGO124034633(hsd17b4)
ONE115111275(hsd17b4)
SALP111974039(hsd17b4)
SNH120045172(hsd17b4)
CCLU121584411(hsd17b4)
ELS105014609(hsd17b4)
SFM108938133(hsd17b4)
PKI111856620(hsd17b4)
AANG118206736(hsd17b4)
LOC102697531(hsd17b4)
PSPA121302596(hsd17b4) 121316706
ARUT117427495 117973220
LCM102356548(HSD17B4)
CMK103181119(hsd17b4)
BFO118409354
BBEL109480804
CIN100187421
SCLV120338122
SPU581580
DMEDmel_CG3415(Mfe2)
DPO4815128
DMN108161558
DGR6565453
RZE108376746 108381954
AGAAgaP_AGAP008667
ACOZ120955687
AARA120901803
ASTE118512713
AAG5568633
AALB109428196
CQUCpipJ_CPIJ001576
CPII120414661
CNS116337519 116349688
BCOO119079223 119084844
NVI100123018
CGLO123268667
FAS105272013
DAM107040917
AGIF122847409
TCA663655(17-beta-HD4)
SOY115888404
CSET123306476
AGB108915760
LDC111505860
APLN108736403
PPYR116158989 116159225 116159796 116159821 116176974 116179454
OTU111423793
BMOR101745499
CFEL113389004
API100167575
DNX107163428
AGS114120233
RMD113555196
DCI103506752 108253294
HHAL106683228
NLU111057072
FOC113212460
TPAL117645519
CSEC111873091
DPXDAPPUDRAFT_332860
DMK116931070
PVM113818099
PJA122257684
PCHN125025607
HAME121875772
PCLA123765087
PTRU123518804
HAZT108681612
EAF111696524
LSM121129872
PPOI119094250 119094251
DSV119440587
RSAN119387719
VDE111252755
VJA111266535
DFR124493442 124496269
SDM118180496
CELCELE_E04F6.3(maoc-1) CELE_M03A8.1(dhs-28)
CBRCBG_10899(Cbr-dhs-28)
LOALOAG_00696
NAINECAME_18174
BGT106065027
GAE121385566
HRF124143730
HRJ124264477
CRG105326295
MYI110453744
PMAX117334218
MMER123566556
OSN115209823
NVE5504006
ATEN116294223
XEN124434950
TADTRIADDRAFT_19721
ATHAT1G76150(ECH2)
ALY9325125
CRB17896162
CSAT104713278 104751510
EUSEUTSA_v10018917mg
BRP103832064
BNA106406002 106421636
BOE106300265
RSZ108817730
THJ104813765
CPAP110818108
CIT102628415
CICCICLE_v10001939mg
PVY116118994
MINC123228653
TCC18591652
GRA105770003
GHI107904979
GAB108453194
DZI111312875 111315264
EGR104448895
GMX100780490 100819304
GSJ114408530 114414554
PVUPHAVU_009G020900g
VRA106762546
VAR108343556
VUN114164056
CCAJ109802448
APRC113864692
MTR25490284
CAM101492992
LJALj1g3v2268360.1(Lj1g3v2268360.1) Lj1g3v2268360.2(Lj1g3v2268360.2)
ADU107460260
AIP107609751
AHF112705683 112766271
LANG109343989
FVE101293547
RCN112174118
PPER18792035
PMUM103321173
PAVI110746650
PDUL117616221
MDM103440214
ZJU107422728
MNT112092488 21394908
CSV101212798
CMO103488374
BHJ120075272
MCHA111014062
CMAX111492280
CMOS111462566
CPEP111806066
RCU8267862
JCU105635268
HBR110636119
MESC110607144 110614577
POP7494669
PEU105137277
PALZ118061960
JRE109018894
QSU112018181 112018301
QLO115974589
TWL119999981
VVI100262653
VRI117907649
SLY101253171
SPEN107005416
SOT102584477
SSTN125863057
CANN107850442
NTA107759031 107773136 107779741 107781501
NSY104216112 104225437
NTO104091855 104109348
NAU109229916 109238023
INI109184502
ITR116032074
SIND105178076
OEU111379149
EGT105950771
SSPL121779755 121782573 121810944
HAN110908076
ECAD122605681
LSV111889535
CCAV112513425
DCR108219134 108219837
CSIN114285416 114307347
BVG104884406
SOE110790608
CQI110703653 110721632
NNU104603702
MING122073830
TSS122657945 122661128
PSOM113289071 113291837
NCOL116253609
OSA4347752
DOSAOs09t0544900-01(Os09g0544900)
OBR102709487 102718952
BDI100844320
ATS109758253
TDC119302740 119311411
TAES123104667 123112928 123122450 123188462
TUA125510275
SBI8054996 8054997
ZMA100272552 103629303 103633156 109942761
SITA101763185 101780628
SVS117844851
PVIR120685902 120696147
PHAI112881184 112882208
PDA103701068
EGU105055161
MUS103970614
DCT110113475
PEQ110023970
AOF109848560
ATR18424521
SMOSELMODRAFT_158543 SELMODRAFT_175507
PPP112281828
SSCKSPSK_04458
NHENECHADRAFT_39459
CFJCFIO01_06283
PFYPFICI_10015
BFUBCIN_11g06000
MBEMBM_09500
GLZGLAREA_11483
CIMCIMG_03533
CPWCPC735_007150
PBNPADG_00601
BGHBDBG_06329
TVSTRAVEDRAFT_75483
DSQDICSQDRAFT_90880
PCOPHACADRAFT_254910
SHSSTEHIDRAFT_55703
HIRHETIRDRAFT_480313
FMEFOMMEDRAFT_108864
LBCLACBIDRAFT_189724(MFP-2)
CCICC1G_06395 CC1G_15551
ABPAGABI1DRAFT114661(AGABI1DRAFT_114661)
ABVAGABI2DRAFT194845(AGABI2DRAFT_194845)
MPRMPER_05878
MRRMoror_12680
SCMSCHCO_02625208(SCHCODRAFT_02625208)
CPUTCONPUDRAFT_58660
WSEWALSEDRAFT_59590
WICJ056_003885
PGRPGTG_04400
MLRMELLADRAFT_85332
MBRMONBRDRAFT_23152
DDIDDB_G0291247(mfeA)
DPPDICPUDRAFT_59190
DFADFA_00521(mfeB)
TGOTGME49_229140
TETTTHERM_00317440 TTHERM_00410270
PTMGSPATT00003144001 GSPATT00004822001
PIFPITG_13257
PSOJPHYSODRAFT_330751
VTAB0498(phaJ)
SPLSpea_1753
SHLShal_1988
SPSWSps_03222
SPSHFM037_25875
FBLFbal_0386
MPURMARPU_08260
AHAAHA_2422
AHYAHML_10855
AHDAI20_09105
AHRV428_11160
AHPV429_11170
AHJV469_12230
AHHRY45_10770
AHIVU14_12155
AAJBOQ57_10160
ASAASA_2281(phaJ)
AEOO23A_p1565
AMEDB224_2076
ADHCK627_15975
ACAVVI35_11530
AEAC2U39_11930
ARVC7N77_13455
AELNCTC12917_02268(phaJ)
ASAAKXJ75_12935
PCAYFRD00_09620
ALENG9X62_11225
 » show all
Reference
1  [PMID:15644212]
  Authors
Koski KM, Haapalainen AM, Hiltunen JK, Glumoff T
  Title
Crystal structure of 2-enoyl-CoA hydratase 2 from human peroxisomal multifunctional enzyme type 2.
  Journal
J Mol Biol 345:1157-69 (2005)
DOI:10.1016/j.jmb.2004.11.009
  Sequence
[hsa:3295]
Reference
2  [PMID:9457873]
  Authors
Fukui T, Shiomi N, Doi Y
  Title
Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae.
  Journal
J Bacteriol 180:667-73 (1998)
DOI:10.1128/JB.180.3.667-673.1998
  Sequence
Reference
3  [PMID:12832794]
  Authors
Koski MK, Haapalainen AM, Hiltunen JK, Glumoff T
  Title
Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2  domain of Candida tropicalis peroxisomal multifunctional enzyme type 2.
  Journal
Acta Crystallogr D Biol Crystallogr 59:1302-5 (2003)
DOI:10.1107/S090744490300982X
Reference
4  [PMID:11134939]
  Authors
Hisano T, Fukui T, Iwata T, Doi Y
  Title
Crystallization and preliminary X-ray analysis of (R)-specific enoyl-CoA hydratase from Aeromonas caviae involved in polyhydroxyalkanoate biosynthesis.
  Journal
Acta Crystallogr D Biol Crystallogr 57:145-7 (2001)
DOI:10.1107/S0907444900014062
Reference
5  [PMID:16982622]
  Authors
Goepfert S, Hiltunen JK, Poirier Y
  Title
Identification and functional characterization of a monofunctional peroxisomal enoyl-CoA hydratase 2 that participates in the degradation of even cis-unsaturated fatty acids in Arabidopsis thaliana.
  Journal
J Biol Chem 281:35894-903 (2006)
DOI:10.1074/jbc.M606383200
  Sequence
[ath:AT1G76150]
Reference
6  [PMID:1879422]
  Authors
Engeland K, Kindl H
  Title
Evidence for a peroxisomal fatty acid beta-oxidation involving D-3-hydroxyacyl-CoAs. Characterization of two forms of hydro-lyase that convert D-(-)-3-hydroxyacyl-CoA into 2-trans-enoyl-CoA.
  Journal
Eur J Biochem 200:171-8 (1991)
DOI:10.1111/j.1432-1033.1991.tb21064.x
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.119
IUBMB Enzyme Nomenclature: 4.2.1.119
ExPASy - ENZYME nomenclature database: 4.2.1.119
BRENDA, the Enzyme Database: 4.2.1.119

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