KEGG   ENZYME: 4.2.1.140
Entry
EC 4.2.1.140                Enzyme                                 
Name
gluconate/galactonate dehydratase;
gluconate dehydratase (ambiguous);
Sso3198 (gene name);
Pto0485 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
D-gluconate/D-galactonate hydro-lyase
Reaction(IUBMB)
(1) D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O [RN:R01538];
(2) D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O [RN:R03033]
Reaction(KEGG)
R01538 R03033
Substrate
D-gluconate [CPD:C00257];
D-galactonate [CPD:C00880]
Product
2-dehydro-3-deoxy-D-gluconate [CPD:C00204];
H2O [CPD:C00001];
2-dehydro-3-deoxy-D-galactonate [CPD:C01216]
Comment
The enzyme is involved in glucose and galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway in Picrophilus torridus [3] and via the branched variant of the Entner-Doudoroff pathway in Sulfolobus solfataricus [1,2]. In vitro it utilizes D-gluconate with 6-10 fold higher catalytic efficiency than D-galactonate [1,3]. It requires Mg2+ for activity [1,2]. cf. EC 4.2.1.6, galactonate dehydratase, and EC 4.2.1.39, gluconate dehydratase.
History
EC 4.2.1.140 created 2013
Pathway
ec00030  Pentose phosphate pathway
ec00052  Galactose metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K05308  gluconate/galactonate dehydratase
Genes
MOPMesop_2573
MAMMesau_05515
MAMOA6B35_24840
MESMEJ066_30325
TROtrd_0911
CCZCCALI_01967
SUSAcid_3439
HALVNG_0442G(rspA)
HSLOE_1664R(gnaD)
HHBHhub_1548(gnaD1)
HAGSJT689_03140
HABOJRZ79_01640
HNOLT974_02950 LT974_08915
HLULT972_10545
HANRLJ422_01860
HJEHacjB3_02665 HacjB3_13980 HacjB3_16911
SALRFQU85_02870
HALREFA46_006425 EFA46_011035
HAADMW046_07370 MW046_14350
HALXM0R89_12595
HAXZM0R88_13190
SALIL593_03520 L593_07320
HARAAArcS_2287(rspA4)
HMArrnAC0575(dgoA4)
HHIHAH_1276
HHNHISP_06530
HABSG26_14175
HTABVU17_06220
HALJG9465_00310 G9465_11295 G9465_19980
HSINKDQ40_07110
NMONmlp_1390(gnaD)
HMUHmuk_0321 Hmuk_2254
HALZE5139_04890 E5139_14415
HALLLC1Hm_1568(gnaD) LC1Hm_2851(dgoD4)
HAZPGBQ70_04885 GBQ70_14430
HALIBV210_12330
HSNDV733_15585
HRRHZS55_02730 HZS55_16360
HPELHZS54_02460 HZS54_19125
HLTI7X12_07425 I7X12_08665 I7X12_10485 I7X12_19535
HARCHARCEL1_10530
HDOMUK72_10685
HWAHQ_2412A(gnaD)
HWCHqrw_2676(gnaD)
HVOHVO_1488(gnaD)
HMEHFX_1553(gnaD)
HGIABY42_07250
HALEG3A49_10595
HLSKU306_12510
HBOHbor_17420
HAJDU500_05985
HAERDU502_13920
HRAEI982_16340
HLMDV707_09645
HALMFCF25_14340
HLAHlac_1300 Hlac_2579
HALPDOS48_06235
HALBEKH57_01910
HEZZEO776_08250
HALQHrr1229_007680 Hrr1229_014835
HSSJ7656_05055 J7656_08185
HSAIHPS36_10285 HPS36_13505
HAZZKI388_05945
SRUBC2R22_00660 C2R22_09090
HAEhalTADL_0374
HALNB4589_014190
HALGHUG10_06105 HUG10_06895 HUG10_06975 HUG10_07030 HUG10_07280 HUG10_11455 HUG10_16095
HALUHUG12_08375 HUG12_08435 HUG12_08585 HUG12_18715
HMPK6T50_02880
HREK6T36_02700
HRMK6T25_11995
HACBHbl1158_11715
HDFAArcSl_1039(gnaD) AArcSl_2834(gnaD2)
HAHHalar_1011 Halar_3730
HTUHtur_2654
HDABB347_08605 BB347_14430 BB347_14735
HSALJMJ58_15575 JMJ58_23050 JMJ58_23365
HLOJ0X27_05005
HAKZJ0X25_01030 J0X25_01075
NMGNmag_0821(gnaD1) Nmag_1145(gnaD2) Nmag_1155(gnaD3) Nmag_3936(gnaD4)
HXAHalxa_4119 Halxa_4131
NATNJ7G_1251
NPENatpe_2049 Natpe_2057
NVRFEJ81_08260 FEJ81_09005 FEJ81_09055 FEJ81_21985
NPLFGF80_03725
NAYHYG81_01505 HYG81_02195 HYG81_23400 HYG81_24285
HALYHYG82_04600 HYG82_13665
HJTDVR14_18340 DVR14_18380 DVR14_22470 DVR14_22510
NGENatgr_0070 Natgr_2179
HRUHalru_1854
NOUNatoc_2547
HLRHALLA_00750 HALLA_06625
HLCCHINAEXTREME08390 CHINAEXTREME10075 CHINAEXTREME10125
NAJB1756_04600
NAGAArcMg_3063
NANAArc1_0639
NBGDV706_11035
NASGCU68_01070 GCU68_01085 GCU68_19305
SAWLNGM29_12820 NGM29_14000
TACTa0085
TVOTVG0179275(TVG0179275)
PTOPTO0485
FACFACI_IFERC01G0930
FAIFAD_1816
CDIVCPM_1948
STOSTK_23660(gnaD)
SOHD1869_12965
SSOSSO3198
SOLSsol_0789 Ssol_0935
SSOASULA_0780 SULA_0968
SSOLSULB_0782 SULB_0970
SSOFSULC_0780 SULC_0969
SSHIJ5U23_00300 J5U23_00504
SCASSACC_30490 SACC_31330
SAISaci_0885
SACNSacN8_04295
SACRSacRon12I_04285
SACSSUSAZ_04035
SISLS215_2325 LS215_2528
SIAM1425_2162 M1425_2356
SIMM1627_2242 M1627_2434
SIDM164_2165 M164_2363
SIYYG5714_2288 YG5714_2500
SINYN1551_0404 YN1551_0627
SIILD85_2428 LD85_2659
SIHSiH_2107 SiH_2298
SIRSiRe_2039 SiRe_2246
SICSiL_2014 SiL_2206
SULABFU36_06085 BFU36_07045
SULEGFS03_11775 GFS03_12675
SULOGFS33_03585 GFS33_04555
SULLEWF20_14025
MSEMsed_1303
MEMJMJ1HA_1511
MCNMcup_0900
MHKDFR87_11980
MPRUDFR88_11805
MTENGWK48_00490
MJNMjAS7_2371
AHOAhos_0261
AMANB6F84_04915
ABRIDFR85_14595 DFR85_15175
ASULDFR86_04375
AAMBD1866_07685
ACIHHS5_15440
SACDHS1genome_0402 HS1genome_1634
SAZOD1868_08675
CSTYKN1_06730
STEPIC006_2132
SAHSHS7_10370
PYRP186_1932
CMACmaq_0154
TTNTTX_1156(gad)
TUZTUZN_0958
VDIVdis_1648 Vdis_2509
VMOVMUT_0856 VMUT_2313
VSOVsou_02030
MIYMicr_00459
 » show all
Reference
1  [PMID:15474024]
  Authors
Lamble HJ, Milburn CC, Taylor GL, Hough DW, Danson MJ
  Title
Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway in Sulfolobus solfataricus.
  Journal
FEBS Lett 576:133-6 (2004)
DOI:10.1016/j.febslet.2004.08.074
  Sequence
[sso:SSO3198]
Reference
2  [PMID:15869466]
  Authors
Ahmed H, Ettema TJ, Tjaden B, Geerling AC, van der Oost J, Siebers B
  Title
The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.
  Journal
Biochem J 390:529-40 (2005)
DOI:10.1042/BJ20041711
  Sequence
[sso:SSO3198]
Reference
3  [PMID:20023024]
  Authors
Reher M, Fuhrer T, Bott M, Schonheit P
  Title
The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate- specific aldolase.
  Journal
J Bacteriol 192:964-74 (2010)
DOI:10.1128/JB.01281-09
  Sequence
[pto:PTO0485]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.140
IUBMB Enzyme Nomenclature: 4.2.1.140
ExPASy - ENZYME nomenclature database: 4.2.1.140
BRENDA, the Enzyme Database: 4.2.1.140

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