KEGG   ENZYME: 4.2.1.157
Entry
EC 4.2.1.157                Enzyme                                 
Name
(R)-2-hydroxyisocaproyl-CoA dehydratase;
2-hydroxyisocaproyl-CoA dehydratase;
HadBC
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(R)-2-hydroxy-4-methylpentanoyl-CoA hydro-lyase
Reaction(IUBMB)
(R)-2-hydroxy-4-methylpentanoyl-CoA = 4-methylpent-2-enoyl-CoA + H2O [RN:R11076]
Reaction(KEGG)
R11076
Substrate
(R)-2-hydroxy-4-methylpentanoyl-CoA [CPD:C21089]
Product
4-methylpent-2-enoyl-CoA [CPD:C21090];
H2O [CPD:C00001]
Comment
The enzyme, isolated from the bacterium Peptoclostridium difficile, is involved in the reductive branch of L-leucine fermentation. It catalyses an alpha/beta-dehydration, which depends on the reductive formation of ketyl radicals on the substrate generated by injection of a single electron from the ATP-dependent activator protein HadI.
History
EC 4.2.1.157 created 2015
Orthology
K20025  (R)-2-hydroxyisocaproyl-CoA dehydratase alpha subunit
K20026  (R)-2-hydroxyisocaproyl-CoA dehydratase beta subunit
Genes
SYMK6K13_14535
PPEEI6G31_11955
CBOCBO2197(hadC) CBO2198(hadB)
CBACLB_2135(hgdB) CLB_2136
CBHCLC_2140 CLC_2141
CBYCLM_2398(hgdB) CLM_2399
CBLCLK_1636 CLK_1637
CBBCLD_2382 CLD_2383
CBICLJ_B2405 CLJ_B2406
CBNCbC4_0487(hgdA) CbC4_0488(hgdB)
CBFCLI_2241 CLI_2242
CBMCBF_2226 CBF_2227
CBJH04402_02206 H04402_02207
CLDCLSPO_c22290 CLSPO_c22300
CTYKCTK_C06860(fldB) CTK_C06870(fldC)
CPRFK7H06_13615 K7H06_13620
PBIFKXZ80_01470 KXZ80_01475
CFEMHCR03_03840
LASAL9O85_02215 L9O85_02220
CASPNQ535_01535 NQ535_01540
CDFCD630_03970(hadB) CD630_03980(hadC)
PDCCDIF630_00525(hadB) CDIF630_00526(hadC)
CDCCD196_0382(hadB) CD196_0383(hadC)
CDLCDR20291_0368(hadB) CDR20291_0369(hadC)
PDFCD630DERM_03970(hadB) CD630DERM_03980(hadC)
PSORRSJ16_16040 RSJ16_16045
ETMCE91St48_25640(hadC) CE91St48_25650(hadB)
AMEILV469_02740 LV469_02745
 » show all
Reference
1  [PMID:15654892]
  Authors
Kim J, Darley D, Buckel W
  Title
2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile.
  Journal
FEBS J 272:550-61 (2005)
DOI:10.1111/j.1742-4658.2004.04498.x
  Sequence
Reference
2  [PMID:21366233]
  Authors
Knauer SH, Buckel W, Dobbek H
  Title
Structural basis for reductive radical formation and electron recycling in (R)-2-hydroxyisocaproyl-CoA dehydratase.
  Journal
J Am Chem Soc 133:4342-7 (2011)
DOI:10.1021/ja1076537
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.157
IUBMB Enzyme Nomenclature: 4.2.1.157
ExPASy - ENZYME nomenclature database: 4.2.1.157
BRENDA, the Enzyme Database: 4.2.1.157

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