KEGG   ENZYME: 4.2.3.21
Entry
EC 4.2.3.21                 Enzyme                                 

Name
vetispiradiene synthase;
vetispiradiene-forming farnesyl pyrophosphate cyclase;
pemnaspirodiene synthase;
HVS;
vetispiradiene cyclase
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, vetispiradiene-forming)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate = vetispiradiene + diphosphate [RN:R06523]
Reaction(KEGG)
R06523
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448]
Product
vetispiradiene [CPD:C12142];
diphosphate [CPD:C00013]
Comment
The initial internal cyclization produces the monocyclic intermediate germacrene A.
History
EC 4.2.3.21 created 2004, modified 2011
Pathway
ec00909  Sesquiterpenoid and triterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K14182  vetispiradiene synthase
Genes
SLY: 101264374(TPS33) 101264676(TPS35)
SPEN: 107030098
SOT: 102577472(PVS2) 102580114 102595695(PVS4)
CANN: 107839748
NTA: 107801757
NSY: 104223404
NTO: 104109674
ITR: 116007323 116023016 116023036 116023069 116023259 116032609
EGT: 105964611 105964660 105964663 105975162 105975175
Reference
1  [PMID:7706281]
  Authors
Back K, Chappell J
  Title
Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases.
  Journal
J Biol Chem 270:7375-81 (1995)
DOI:10.1074/jbc.270.13.7375
  Sequence
Reference
2
  Authors
Keller, H., Czernic, P., Ponchet, M., Ducrot, P.H., Back, K., Chappell, J., Ricci, P. and Marco, Y.
  Title
Sesquiterpene cyclase is not a determining factor for elicitor- and pathogen-induced capsidiol accumulation in tobacco.
  Journal
Planta 205:467-476 (1998)
Reference
3  [PMID:9204881]
  Authors
Mathis JR, Back K, Starks C, Noel J, Poulter CD, Chappell J.
  Title
Pre-steady-state study of recombinant sesquiterpene cyclases.
  Journal
Biochemistry 36:8340-8 (1997)
DOI:10.1021/bi963019g
Reference
4  [PMID:10588069]
  Authors
Yoshioka H, Yamada N, Doke N.
  Title
cDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans.
  Journal
Plant Cell Physiol 40:993-8 (1999)
DOI:10.1093/oxfordjournals.pcp.a029633
  Sequence
[sot:102595695]
Reference
5  [PMID:11745124]
  Authors
Martin VJ, Yoshikuni Y, Keasling JD.
  Title
The in vivo synthesis of plant sesquiterpenes by Escherichia coli.
  Journal
Biotechnol Bioeng 75:497-503 (2001)
DOI:10.1002/bit.10037
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.21
IUBMB Enzyme Nomenclature: 4.2.3.21
ExPASy - ENZYME nomenclature database: 4.2.3.21
BRENDA, the Enzyme Database: 4.2.3.21
CAS: 192465-18-2

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