Entry Name 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase;
Class Lyases;BRITE hierarchy
Sysname 5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate pyruvate-lyase [(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate-forming]
Reaction(IUBMB) 5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate [RN:
R08166 ]
Reaction(KEGG) Substrate 5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate [CPD:
C16519 ]
Product (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate [CPD:
C05817 ];
pyruvate [CPD:
C00022 ]
Comment This enzyme is involved in the biosynthesis of vitamin K2 (menaquinone). In most anaerobes and all Gram-positive aerobes, menaquinone is the sole electron transporter in the respiratory chain and is essential for their survival. It had previously been thought that the reactions carried out by this enzyme and EC
2.2.1.9 , 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase, were carried out by a single enzyme but this has since been disproved [2].
History EC 4.2.99.20 created 2008 (EC 2.5.1.64 created 2003, part-incorporated 2008)
Pathway ec00130 Ubiquinone and other terpenoid-quinone biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology K08680 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
K14759 isochorismate synthase / 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase / 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / o-succinylbenzoate synthase
Genes CSAT : 104704752 104750694 104787948
PSOM : 113275718 113281895
TAES : 123127572 123137052 123144651
PVIR : 120656211 120706507
SMO : SELMODRAFT_121498 SELMODRAFT_157803
ECOO : ECRM13514_3020(yfbB)
ECOH : ECRM13516_2965(yfbB)
EDJ : ECDH1ME8569_2200(yfbB)
ECOI : ECOPMV1_02425(menH)
RTG : NCTC13098_02011(menH)
REE : electrica_01486(menH)
CLAP : NCTC11466_01326(menH)
KIE : NCTC12125_00420(menH)
AHN : NCTC12129_01547(yfbB)
METY : MRY16398_16350(menH)
PSHI : SAMEA2665130_2006(menH)
PSGC : G163CM_03900(menH_3)
SMW : SMWW4_v1c33920(menH)
SFJ : SAMEA4384070_3398(menH)
SOF : NCTC11214_03404(menH)
PAQU : DMB82_0014820(menH)
DIC : Dpoa569_002644(menH)
MTHI : C7M52_00764(menH_1)
PHEI : NCTC12003_01572(menH)
PRJ : NCTC6933_02322(menH)
LRI : NCTC12151_01276(menH)
HIC : NTHIC486_00706(menH)
HPIT : NCTC13334_01273(menH)
HHZ : NCTC10839_00884(menH)
HAEG : NCTC8502_00939(menH)
PDAG : 4362423_00351(menH)
ADP : NCTC12871_00402(menH)
ALIG : NCTC10568_02125(menH)
ASEG : NCTC10977_00866(menH)
AVT : NCTC3438_01960(menH)
RPNE : NCTC8284_01143(menH)
PAET : NCTC13378_01484(menH)
VAU : VANGNB10_cI1810c(ytxM_menH?)
VPL : SA104470976_01679(menH)
SCAA : TUM17387_36810(menH)
TNI : TVNIR_1662(menH_[H])
AEL : NCTC12917_00432(menH)
CDIZ : CEDIAZO_00455(menH_1)
BAZ : BAMTA208_15080(ytxM)
BSON : S101395_01270(menH)
BFD : NCTC4823_01167(menH)
PARG : PspKH34_06270(menH)
BLEN : NCTC4824_03197(menH)
SUQ : HMPREF0772_12190(menH)
SDP : NCTC12225_02004(menH)
SPIC : SAMEA4384060_1911(menH)
SSH : NCTC13712_00990(menH)
SSIM : SAMEA4384339_0959(menH)
SSTE : SAMEA4384403_1818(menH)
ECEC : NCTC12421_02103(menH)
VRM : 44547418_00396(menH)
VDN : NCTC11831_00357(menH)
BALA : DSM104299_02591(menH_1)
RTS : CE91St31_31150(menH)
NSPH : BDGGKGIB_02556(menH)
PROC : Ptc2401_01967(menH)
» show all Taxonomy Reference Authors Jiang M, Chen X, Guo ZF, Cao Y, Chen M, Guo Z.
Title Identification and Characterization of (1R,6R)-2-Succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate Synthase in the Menaquinone Biosynthesis of Escherichia coli.
Journal Sequence Reference Authors Jiang M, Cao Y, Guo ZF, Chen M, Chen X, Guo Z.
Title Menaquinone biosynthesis in Escherichia coli: identification of 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate as a novel intermediate and re-evaluation of MenD activity.
Journal Sequence Other DBs ExPASy - ENZYME nomenclature database: 4.2.99.20
