Lyases;
Carbon-nitrogen lyases;
Amine-lyases
BRITE hierarchy

D-ribose 5-phosphate,D-glyceraldehyde 3-phosphate pyridoxal 5'-phosphate-lyase

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate (overall reaction) [RN:R10089];
(1a) L-glutamine + H2O = L-glutamate + NH3 [RN:R00256];
(1b) D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3 = pyridoxal 5'-phosphate + 4 H2O + phosphate [RN:R10088]

R00256 R10088 R10089;
(other) R07456
Reaction

D-ribose 5-phosphate [CPD:C00117];
D-glyceraldehyde 3-phosphate [CPD:C00118];
L-glutamine [CPD:C00064];
H2O [CPD:C00001];
NH3 [CPD:C00014]

The ammonia is provided by the glutaminase subunit and channeled to the active site of the lyase subunit by a 100 A tunnel. The enzyme can also use ribulose 5-phosphate and dihydroxyacetone phosphate. The enzyme complex is found in aerobic bacteria, archaea, fungi and plants.

EC 4.3.3.6 created 2011