KEGG   ENZYME: 5.5.1.27
Entry
EC 5.5.1.27                 Enzyme                                 
Name
D-galactarolactone cycloisomerase;
GCI
Class
Isomerases;
Intramolecular lyases;
Intramolecular lyases (only sub-subclass identified to date)
Sysname
D-galactaro-1,4-lactone lyase (ring-opening)
Reaction(IUBMB)
(1) D-galactaro-1,4-lactone = 5-dehydro-4-deoxy-D-glucarate [RN:R10847];
(2) D-glucaro-1,4-lactone = 5-dehydro-4-deoxy-D-glucarate [RN:R11083]
Reaction(KEGG)
R10847 R11083
Substrate
D-galactaro-1,4-lactone [CPD:C20896];
D-glucaro-1,4-lactone [CPD:C21095]
Product
5-dehydro-4-deoxy-D-glucarate [CPD:C00679]
Comment
The enzyme, characterized from the bacterium Agrobacterium fabrum strain C58, is involved in degradation of D-galacturonate and D-glucuronate. Activity with D-galactaro-1,4-lactone is 4-fold higher than with D-glucaro-1,4-lactone.
History
EC 5.5.1.27 created 2015
Pathway
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
Orthology
K18983  D-galactarolactone cycloisomerase
Genes
CAMAF384_17470
CTELGBC03_28085
IZHFEM41_12900
GQUAWC35_04195
SODSant_1026 Sant_2261
SLIGGTU79_12705
PAOPat9b_4431
KLNLH22_06000
PANSFCN45_23420
PSIS70_13550
PSXDR96_551
PSTABGK56_16010
PRGRB151_022700(rhmD)
PRQCYG50_05295
PVCG3341_16965
VNIVIBNI_B0980
VSCVSVS12_03474(lra3)
VAFD1115_10435
VASGT360_19110
PGBH744_1c0304
SPLSpea_3858
SHLShal_0409
SJASJ2017_1939
COLWA3Q33_18040
THTE2K93_07385
PATPatl_0897
PBWD172_007745
PCARPC2016_3905
PMESFX988_01827
CATEC2869_00665
FBLFbal_0608
MTHDA3224_10175
MAGAMag101_05735
SROSBBH56_04565
GAIIMCC3135_03235(gci_1) IMCC3135_28780(gci_2)
CSACsal_2476
HELHELO_3407(gci)
HAKKO116_03370
HAMHALO3643
HOLHORIV_64370
HTTHZS52_07800
HCAMI4484_00185
HSXHNO51_00185
HSVHNO53_00185
HTXEKK97_00190
KUYFY550_00795
ADIB5T_03504
MMEMarme_0065
MPCMar181_2906
MARSA8C75_18900
LLPGH975_04805 GH975_07765
SALNSALB1_0470
CGDCR3_3860
PGPCUJ91_31590
PTROG5S35_31625
PACSFAZ98_10555 FAZ98_30730
BPARBN117_4287
BPABPP1390
BBHBN112_0281 BN112_3736
BBRBB2459 BB4687
BBMBN115_2658
BBXBBS798_2295 BBS798_4463
BPTBpet2903
BBROBAU06_00140 BAU06_14035 BAU06_14065 BAU06_22040
BFZBAU07_12100 BAU07_12145 BAU07_22265
BOHAKI39_13245 AKI39_13285 AKI39_13430
PUSCKA81_13890
AKATKWG_00290
ODIODI_R1804
PARKLSG25_04810
VAPVapar_5519
VPDVAPA_2c05200
DRGH9K76_08570
XYKGT347_26065
ATWC0099_01355
DFIAXF13_00735
MESWA9K65_013755
MESPC1M53_17420
MESMeso_1946
RPODE0E05_16250
NKIKW403_06520
RHINGR_b14290
SFHSFHH103_05618
EADOV14_a1863 OV14_b0357
EAHFA04_25485 FA04_30525
EMXFKV68_21235
ATUAtu3139
ATAAWN88_10710
AGRAGROH133_10632
ATFAch5_29940(gci)
AGCBSY240_2211
AROB0909_22900
AGTEYD00_14440
ALFCFBP5473_09270
ARUIG6M88_13790
AVQHRR99_06015
RLBRLEG3_27710
RIRBN877_II0123
RPUSCFBP5875_14590
RHVBA939_26325
RJGCCGE525_24245 CCGE525_30040
RHRCKA34_27150 CKA34_27745
ROYG3A56_17325
RIIFFM53_017765
RROSD4A92_09815
NGLRG1141_CH22490
NGGRG540_CH23330
NEOCYG48_08760
NENNCHU2750_21870(gci) NCHU2750_42880 NCHU2750_47880 NCHU2750_53580
SHZshn_16860 shn_28795
SZOK8M09_21620
SOJK6301_22790
PDESFE840_018515
BJUBJ6T_69710
BJPRN69_33820
BRSS23_45390
BRCBCCGELA001_12525
BRADBF49_1895
BICLMTR13_05250 LMTR13_17465
BOTCIT37_35350
BRQCIT40_11260
BGQX265_26885
BSYMCIT39_06340 CIT39_10370
BARHWN72_15330
BBANJ4G43_016105
RPBRPB_4061
RBKE0H22_13950
VGOGJW-30_1_00576(rhmD)
BOPAXW83_04155 AXW83_25440 AXW83_26310
BVVBHK69_06615 BHK69_14610
BOIBLM15_17215 BLM15_23225
BOFFQV39_20260
XAUXaut_3370
XDIEZH22_23385
SNOSnov_4076 Snov_4090
STARG3545_11175 G3545_16275 G3545_16295 G3545_27185
LNEFZC33_19190
APRAG3A50_04735
MMESMMSR116_04700
MOCBB934_24410
CHELAL346_24405
CDQBOQ54_18660
PHLKKY_3040
DEQXM25_20980
DEAFPZ08_20120
YTIFNA67_02305
JIEOH818_20375
NOHG5V57_20170
PSTGE8M01_20230
ACUTMRB58_08345 MRB58_11990
SILSPO2414
RUAD1823_06565
RUTFIU92_10665(yitF)
RCONK3740_04780
RDERD1_0679
RLIRLO149_c038620
OATOAN307_c19320
OAROA238_c47540 OA238_c47610
OCTFTO60_03720
MALGMALG_00963 MALG_02603
YPACCEW88_05335
YANAYJ57_18565
TPROGa0080559_TMP359 Ga0080559_TMP419
SULZC1J03_24785
SPSESULPSESMR1_04919(gci)
AHTANTHELSMS3_02587
RSHRsph17029_4153
PTPRCA23_c23230 RCA23_c23350
RHCRGUI_3938
LITFPZ52_00260
PPRUFDP22_06925
PSHQF3W81_11305
PALWPSAL_035960(gci)
HDHG5B40_00590 G5B40_03710 G5B40_08685
CMAGCBW24_05885
SSANNX02_16030
SPLMBXU08_01130
SPHCCVN68_11675
SPHLLPB140_03495
EGNBMF35_a0049 BMF35_a0055 BMF35_a0333
PHZCHX26_04735
SRFLHU95_06515
RAPRHOA_4870
SHUMSTHU_30510(gci_2) STHU_32630
SVCSTVA_18940 STVA_20350(gci_2)
STELSTAQ_17870(gci) STAQ_29340
ABSAZOBR_p310126
ABQABAZ39_25585
AZTTSH58p_01545
AARED3093_24830
NACINUH88_05275
BSSBSUW23_05560(yitF)
BSTGYO_1403(yitF)
BLIBL01977(yitF)
BLDBLi02157(yitF)
BLHBaLi_c22320(yitF)
BAEBATR1942_03120
BGYBGLY_2291
BITBIS30_16195
BBEBBR47_38150(yitF)
BLRBRLA_c034680(yitF)
BFMBP422_14390
BAGRBA6348_12065
BRWGOP56_09425
BCHSJNE38_19360
PNPIJ22_01170 IJ22_15690
PALOE6C60_0203
NTRB0W44_15605
KPULGXN76_07985
PABSJIR001_24760(yitF)
DRUDesru_2534
TFRBR63_01905 BR63_13330
FWADCMF_06330
VPYHZI73_07910
GEKkuro4_13840(gci)
LPILLIP_0420 LIP_0509 LIP_1982
SVLStrvi_4313
SRCM271_03750
SMALSMALA_7628
SBHSBI_00752
SXISXIM_20370
SCHACP983_06225
SGXH4W23_01580
SHARHUT13_08940
MOYCVS54_01196(gci)
AGYATC03_14870
SALAESZ53_09205
HEAHL652_18515
ARZAUT26_19340
ARNCGK93_10790
ARXARZXY2_4041
PUEFV140_21085
GARAOZ07_00220
SATKSA2016_0899
BCVBcav_0644
BRXBH708_16440 BH708_16790
BGGCFK41_13980
CETB8281_01575
MIKFOE78_04480
MICGGJV80_15955
PSIMKR76_00300
KFLKfla_2398
NOABKM31_21020
NAKEH165_12545
SENSACE_2582
PAUTPdca_64030
ACTIUA75_20035
ACADUA74_19545
PMADBAY61_16475
MTEMGCE86_03285
PSUUPsuf_090430
ATLAthai_20320 Athai_45540
PVSA1sIA79_01995 A1sIA79_06480
ERZER308_01805 ER308_15350
TTRTter_2214
VBSEGM51_06115
AAGGETAA8_24950(rhmD)
AMOBHG15A2_17800(yitF)
PLSVT03_23450(yitF)
PLHVT85_12270(yitF)
FMRFuma_05789(yitF)
GMRGmarT_18570(yitF)
MRIMal4_04010
SDYNMal52_00340(yitF)
GESVT84_20960(rhmD)
GMSSOIL9_12620
TIMGMBLW1_25140
ULIETAA1_22390(rhmD) ETAA1_55000(dgoD_4)
SACISinac_0917
AGVOJF2_00180(yitF)
PFERIRI77_24375
ABACLuPra_02649(dgoD_3)
MCOSGM418_07785
MBASALGA_2221
CHITFW415_11695
COYHF329_06185
HHYHalhy_2207
PGSCPT03_08685
SHTKO02_11610 KO02_15740
SPHNBV902_21785
STHANCTC11429_00215
MMABHQ865_19795
SBXCA265_12255
CMRCycma_3933
EOLEmtol_1245
MAQALAG90_11920
FLMMY04_5029 MY04_5048
FLLEI427_16820 EI427_21965
FYAKMW28_27615 KMW28_27710
FKAKM029_22790 KM029_26030
FFAFFWV33_17870
MARMYQ22_09895
MARTBTR34_09960
MARBCJ263_06230
MAREEJ994_11795
CAOCelal_0417
CLYCelly_1807
CLHIX49_08920
CBALM667_19555
CBATM666_19475
ZGAZOBELLIA_3156(dgoD)
PUNNQP51_16915
TJETJEJU_2926
WFUAXE80_10120
FOHFORMA_02450
TAJC1A40_08335
KANIMCC3317_46240(gci)
GAAHX109_10180
FBEFF125_03875
HALXM0R89_21380
HALJG9465_20630
HPELHZS54_10535
HGIABY42_15285
HAQDU484_06805
HAJDU500_00255 DU500_07075
HAERDU502_04740
HRAEI982_05865 EI982_09005
HALQHrr1229_004955
SRUBC2R22_00735
HALGHUG10_06970 HUG10_07125 HUG10_11805 HUG10_15365
HALUHUG12_05215 HUG12_08520 HUG12_17675
HMPK6T50_03860
HREK6T36_17035
HTUHtur_4435
HSALJMJ58_23585
HXAHalxa_0385
NOUNatoc_1246
HLRHALLA_00610 HALLA_04440
VDIVdis_0390
 » show all
Reference
1  [PMID:22493433]
  Authors
Andberg M, Maaheimo H, Boer H, Penttila M, Koivula A, Richard P
  Title
Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate.
  Journal
J Biol Chem 287:17662-71 (2012)
DOI:10.1074/jbc.M111.335240
  Sequence
[atu:Atu3139]
Reference
2  [PMID:24450804]
  Authors
Bouvier JT, Groninger-Poe FP, Vetting M, Almo SC, Gerlt JA
  Title
Galactaro delta-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily.
  Journal
Biochemistry 53:614-6 (2014)
DOI:10.1021/bi5000492
  Sequence
[atu:Atu3139]
Other DBs
ExplorEnz - The Enzyme Database: 5.5.1.27
IUBMB Enzyme Nomenclature: 5.5.1.27
ExPASy - ENZYME nomenclature database: 5.5.1.27
BRENDA, the Enzyme Database: 5.5.1.27

DBGET integrated database retrieval system