KEGG   ENZYME: 6.1.1.26
Entry
EC 6.1.1.26                 Enzyme                                 
Name
pyrrolysine---tRNAPyl ligase;
PylS;
pyrrolysyl-tRNA synthetase
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
Sysname
L-pyrrolysine:tRNAPyl ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-pyrrolysine + tRNAPyl = AMP + diphosphate + L-pyrrolysyl-tRNAPyl [RN:R08578]
Reaction(KEGG)
R08578
Substrate
ATP [CPD:C00002];
L-pyrrolysine [CPD:C16138];
tRNA(Pyl) [CPD:C16139]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-pyrrolysyl-tRNA(Pyl) [CPD:C17027]
Comment
In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNAPyl, with a CUA anticodon, can be charged directly with pyrrolysine by this class II aminoacyl---tRNA ligase. The enzyme is specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids [1].
History
EC 6.1.1.26 created 2007
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K11627  pyrrolysyl-tRNA synthetase
Genes
MBAMbar_A0839
MBYMSBRM_0155
MBWMSBRW_0162
MBARMSBR2_2226
MBAKMSBR3_2277
MACMA_0155
MMAMM_1445
MMJMSMAS_3219
MMACMSMAC_3241
MVCMSVAZ_3361
MEKMSKOL_3320
MLSMSLAZ_3211
METMMSMTP_3119
MEFMSWH1_3068
MEQMSWHS_3279
MSJMSSAC_0137
MSZMSSIH_0124
MSWMSSIT_0128
MTHRMSTHT_1934
MTHEMSTHC_1349
MHORMSHOH_3965
MFZAOB57_008970
MBUMbur_2086
MMETMCMEM_0285
MELOJ7W08_11520
MMHMmah_0283
MHAZBHR79_02105(pylS)
MEVMetev_0309
MZHMzhil_1025
MPYMpsy_1721(pylS)
MZIHWN40_00965(pylS)
MHZMetho_0022
 » show all
Reference
1  [PMID:15329732]
  Authors
Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA.
  Title
Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.
  Journal
Nature 431:333-5 (2004)
DOI:10.1038/nature02895
  Sequence
Reference
2  [PMID:15314242]
  Authors
Polycarpo C, Ambrogelly A, Berube A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Soll D
  Title
An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.
  Journal
Proc Natl Acad Sci U S A 101:12450-4 (2004)
DOI:10.1073/pnas.0405362101
  Sequence
Reference
3  [PMID:15372017]
  Authors
Schimmel P, Beebe K.
  Title
Molecular biology: genetic code seizes pyrrolysine.
  Journal
Nature 431:257-8 (2004)
DOI:10.1038/431257a
Other DBs
ExplorEnz - The Enzyme Database: 6.1.1.26
IUBMB Enzyme Nomenclature: 6.1.1.26
ExPASy - ENZYME nomenclature database: 6.1.1.26
BRENDA, the Enzyme Database: 6.1.1.26

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