KEGG   ENZYME: 6.2.1.66
Entry
EC 6.2.1.66                 Enzyme                                 
Name
glyine---[glycyl-carrier protein] ligase;
dhbF (gene name);
sfmB (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
glycine:[glycyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + glycine + holo-[glycyl-carrier protein] = AMP + diphosphate + glycyl-[glycyl-carrier protein] (overall reaction);
(1a) ATP + glycine = diphosphate + (glycyl)adenylate [RN:R12721];
(1b) (glycyl)adenylate + holo-[glycyl-carrier protein] = AMP + glycyl-[glycyl-carrier protein]
Reaction(KEGG)
R12721;
(other) R12722 R12723
Substrate
ATP [CPD:C00002];
glycine [CPD:C00037];
holo-[glycyl-carrier protein];
(glycyl)adenylate [CPD:C22361]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
glycyl-[glycyl-carrier protein];
(glycyl)adenylate [CPD:C22361]
Comment
The adenylation domain of the enzyme catalyses the activation of glycine to (glycyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein (as in the case of DhbF in bacillibactin biosynthesis), or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.66 created 2021
Pathway
ec01053  Biosynthesis of siderophore group nonribosomal peptides
Orthology
K04780  glyine---[glycyl-carrier protein] ligase
Genes
BRHRBRH_00578
VPEVarpa_4519
VPDVAPA_1c40300
JAGGJA_3056(dhbF)
BSUBSU31960(dhbF)
BSRI33_3287
BSLA7A1_2308
BSHBSU6051_31960(dhbF)
BSYI653_15395
BSUTBSUB_03414(dhbF)
BSULBSUA_03414(dhbF)
BSUSQ433_17480(dhbF)
BSOBSNT_09659(dhbF)
BSNBSn5_06870
BSQB657_31960(dhbF)
BSXC663_3055
BSPU712_15925
BSSBSUW23_15540(dhbF)
BSTGYO_3487
BLIBL04024(dhbF)
BLDBLi03898(dhbF)
BLHBaLi_c39150(dhbF)
BAYRBAM_029010
BAQBACAU_2929(dhbF)
BYABANAU_3090(dhbF)
BAMPB938_14845(dhbF)
BQYMUS_3488(dhbF)
BAMLBAM5036_2817(dhbF)
BAMARBAU_3032(dhbF)
BAMNBASU_2823(dhbF)
BAMBBAPNAU_3080(dhbF)
BAMTAJ82_16420
BAMYV529_31610(dhbF)
BMPNG74_03051(dhbF)
BAOBAMF_2992(dhbF)
BAZBAMTA208_15880(dhbF)
BQLLL3_03265(dhbF)
BXHBAXH7_03248(dhbF)
BAMIKSO_004640
BAMCU471_30070
BAMFU722_15635
BSIACWD84_04845
BAEBATR1942_13800
BVMB9C48_14825
BSONS101395_00491(entF)
BHTDIC78_15210
BMOJHC660_30810(dhbF)
BTEQG4P54_16295
BIQAN935_16035
BANBA_2372(dhbF)
BARGBAA_2372(dhbF)
BATBAS2208
BAHBAMEG_2228(bacF)
BAIBAA_2430(bacF)
BAXH9401_2251
BANTA16_24050
BANRA16R_24320
BANSBAPAT_2265 BAPAT_2266
BANVDJ46_1161(dltA)
BCABCE_2402(dhbF)
BCZBCE33L2131(entF)
BCRBCAH187_A2472(bacF)
BCBBCB4264_A2337(bacF)
BCUBCAH820_2390(bacF)
BCGBCG9842_B2988(bacF)
BCQBCQ_2298(dhbF)
BCXBCA_2439(bacF)
BALBACI_c23170(rimI5)
BNCBCN_2293
BCFbcf_11800
BCERBCK_23055
BCEFBcrFT9_01878 BcrFT9_01879 BcrFT9_01880
BCYBcer98_1758
BTKBT9727_2147(entF)
BTLBALH_2111(entF)
BTBBMB171_C2077(bacF)
BTTHD73_2593
BTHRYBT1520_12705
BTCCT43_CH2269(bacF)
BTFYBT020_12045
BTMMC28_1578
BTGBTB_c23880(dhbF)
BTIBTG_08300
BTNBTF1_09045
BTHTH175_ch2305
BTHUYBT1518_12780
BTWBF38_3557(dltA)
BTHYAQ980_18085
BWEBcerKBAB4_2179
BWWbwei_2663(dltA3)
BMYOBG05_3688(dltA)
BTYBtoyo_4934
BMYCDJ92_4947(dltA)
BBYCY96_10655
BWDCT694_12505
BTROFJR70_07850
BMOBMLA2C4_12210
BNTGSN03_11560
BPACLMD38_14960
BPUSUP12_18995
BGYBGLY_4305(dhbF)
BALTCFN77_19550
BSAFBSL056_20115
BITBIS30_05510
BCABEFK13_16390
BRYM0696_16090(dhbF)
BJSMY9_3208
GSTHW35_12765
BACWQR42_18505
BACPSB24_14220
BACBOY17_17830
BACYQF06_14305
BACLBS34A_34930(dhbF)
BALMBsLM_3191
BACSAUL54_15200
BEOBEH_16975
BGIBGM20_10080
BKOCKF48_20580
OIHOB0958(dhbF)
GMCGY4MC1_0171
GTHGeoth_0191
PTLAOT13_02025
ANMGFC28_2443(dltA)
ANLGFC29_2992(dltA)
ANDGRQ40_01370
ACAIISX45_04580
ASEDIRT44_08355
BMURABE28_001170
PPSRI6J18_13150
SCIBHUG20_17410
SCIAHUG15_20215
SLMSMM221_10930
GYMGYMC10_4573
PLVERIC2_c15760(dhbF)
PIHUB51_13750
PKBB4V02_18060
PLWD5F53_07335
PLENEIM92_18440
PCHIPC41400_19010
PCELHUB94_23720
TABCIG75_13300
SIVSSIL_0953
SSILSOLI23_13850
VIJJNUCC6_01520
PFTJBW_01674
MSHOMSHO_41470
CPRECsp1_26920(dhbF_2)
CUWLH390_08965
NHUH0264_13950
RHARHA1_ro02319
ROPROP_20370
ROAPd630_LPD06513
RKOJWS14_12085
ROZCBI38_26795
TPRTpau_3693
TPULTPB0596_40260(dhbF_1)
SGRSGR_6739
SGBWQO_01320
SFISFUL_387
SCZABE83_33725
SALWCP975_14820
SCADDN051_01415
SALJSMD11_0929(dhbF)
SFKKY5_3423
SKACP970_25135
SRKFGW37_27510
SCAVCVT27_00785
SRIMCP984_09395
SCOECP976_03610
SBATG4Z16_05985
SDWK7C20_29975
SCALI6J39_01025
SFBCP974_29335
SPEUCGZ69_27080
SGJIAG43_29410
KABB7C62_00825
RTERIDM49_03310
 » show all
Reference
1  [PMID:11112781]
  Authors
May JJ, Wendrich TM, Marahiel MA
  Title
The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2,3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin.
  Journal
J Biol Chem 276:7209-17 (2001)
DOI:10.1074/jbc.M009140200
  Sequence
[bsu:BSU31960]
Reference
2  [PMID:17981978]
  Authors
Li L, Deng W, Song J, Ding W, Zhao QF, Peng C, Song WW, Tang GL, Liu W
  Title
Characterization of the saframycin A gene cluster from Streptomyces lavendulae NRRL 11002 revealing a nonribosomal peptide synthetase system for assembling the unusual tetrapeptidyl skeleton in an iterative manner.
  Journal
J Bacteriol 190:251-63 (2008)
DOI:10.1128/JB.00826-07
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.66
IUBMB Enzyme Nomenclature: 6.2.1.66
ExPASy - ENZYME nomenclature database: 6.2.1.66
BRENDA, the Enzyme Database: 6.2.1.66

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