KEGG   ENZYME: 6.2.1.7
Entry
EC 6.2.1.7                  Enzyme                                 
Name
cholate---CoA ligase;
BAL;
bile acid CoA ligase;
bile acid coenzyme A ligase;
choloyl-CoA synthetase;
choloyl coenzyme A synthetase;
cholic thiokinase;
cholate thiokinase;
cholic acid:CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl coenzyme A synthetase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA synthetase;
THCA-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate---CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate:CoA ligase (AMP-forming);
cholyl-CoA synthetase;
trihydroxycoprostanoyl-CoA synthetase
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
cholate:CoA ligase (AMP-forming)
Reaction(IUBMB)
(1) ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA [RN:R02794];
(2) ATP + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA [RN:R04580]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
cholate [CPD:C00695];
CoA [CPD:C00010];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
choloyl-CoA [CPD:C01794];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA
Comment
Requires Mg2+ for activity. The mammalian enzyme is membrane-bound and catalyses the first step in the conjugation of bile acids with amino acids, converting bile acids into their acyl-CoA thioesters. Chenodeoxycholate, deoxycholate, lithocholate and trihydroxycoprostanoate can also act as substrates [7]. The bacterial enzyme is soluble and participates in an anaerobic bile acid 7 alpha-dehydroxylation pathway [5].
History
EC 6.2.1.7 created 1961 (EC 6.2.1.29 created 1992, incorporated 2005), modified 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec00121  Secondary bile acid biosynthesis
ec01100  Metabolic pathways
Orthology
K08748  solute carrier family 27 (fatty acid transporter), member 5
K15868  bile acid-coenzyme A ligase
Genes
HSA10998(SLC27A5)
PTR456351(SLC27A5)
PPS100973518(SLC27A5)
GGO101141402(SLC27A5)
PON100450572(SLC27A5)
NLE100598523(SLC27A5)
MCC713713(SLC27A5)
MCF102139611(SLC27A5)
CSAB103235458(SLC27A5)
CATY105593284(SLC27A5)
PANU101012361(SLC27A5)
TGE112612525(SLC27A5)
RRO104680867(SLC27A5)
RBB108514368(SLC27A5)
TFN117091868(SLC27A5)
PTEH111530345(SLC27A5)
CJC100394591(SLC27A5)
SBQ101033269(SLC27A5)
CSYR103262310(SLC27A5)
MMUR105864502(SLC27A5)
LCAT123624765(SLC27A5)
OGA100943998(SLC27A5)
MMU26459(Slc27a5)
MCAL110297223(Slc27a5)
MPAH110336881(Slc27a5)
RNO79111(Slc27a5)
MCOC116101654(Slc27a5)
MUN110562939(Slc27a5)
CGE100765567(Slc27a5)
MAUA101824762(Slc27a5)
PLEU114703883(Slc27a5)
MORG121449385(Slc27a5)
AAMP119820463(Slc27a5)
NGI103725476(Slc27a5)
HGL101724180(Slc27a5)
CPOC100717817(Slc27a5)
CCAN109683037(Slc27a5)
DORD105994312(Slc27a5)
DSP122110673(Slc27a5)
NCAR124966722
OCU100340287(SLC27A5)
OPI101527096(SLC27A5)
TUP102483776(SLC27A5)
CFA608675(SLC27A5)
CLUD112643327(SLC27A5)
VVP112908791(SLC27A5)
VLG121484156(SLC27A5)
AML100478693(SLC27A5)
UMR103677216(SLC27A5)
UAH113248017(SLC27A5)
UAR123776624(SLC27A5)
ELK111139861
LLV125089011
MPUF101687342(SLC27A5)
ORO101381954(SLC27A5)
EJU114200965(SLC27A5)
ZCA113936449(SLC27A5)
MLX117997921(SLC27A5)
FCA101086686(SLC27A5)
PYU121023395(SLC27A5)
PBG122494649(SLC27A5)
PTG102948587(SLC27A5)
PPAD109260425(SLC27A5)
AJU106987933(SLC27A5)
HHV120227093(SLC27A5)
BTA533016(SLC27A5)
BOM102285844(SLC27A5)
BIU109571897(SLC27A5)
BBUB102396712(SLC27A5)
CHX102172839(SLC27A5)
OAS101120689(SLC27A5)
ODA120871847(SLC27A5)
CCAD122420603(SLC27A5)
SSC102158419(SLC27A5)
CFR102519177(SLC27A5)
CBAI105074455(SLC27A5)
CDK105096797(SLC27A5)
VPC102538658(SLC27A5)
BACU103020672(SLC27A5)
LVE103086085(SLC27A5)
OOR101280268(SLC27A5)
DLE111170038(SLC27A5)
PCAD102979842(SLC27A5)
PSIU116744807(SLC27A5)
ECB100051678(SLC27A5)
EPZ103567891(SLC27A5)
EAI106846663(SLC27A5)
MYB102261821(SLC27A5)
MYD102751593(SLC27A5)
MMYO118657417(SLC27A5)
MLF102425294(SLC27A5)
MNA107539367(SLC27A5)
PKL118712387(SLC27A5)
HAI109377097(SLC27A5)
DRO112313581(SLC27A5)
SHON118988175(SLC27A5)
AJM119051596(SLC27A5)
PDIC114511678(SLC27A5)
PHAS123825485(SLC27A5)
MMF118638221(SLC27A5)
RFQ117034578(SLC27A5)
PALE102890200(SLC27A5)
PGIG120607133(SLC27A5)
PVP105308648(SLC27A5)
RAY107500402(SLC27A5)
MJV108408237(SLC27A5)
TOD119249800(SLC27A5)
SARA101552573(SLC27A5)
LAV100676206(SLC27A5)
TMU101361902
DNM101438816(SLC27A5)
MDO100029941(SLC27A5)
GAS123242899(SLC27A5)
SHR100916639(SLC27A5)
PCW110220184(SLC27A5)
OAA100090562(SLC27A5)
KIMG3T16_11475 G3T16_16845
SDFACG33_01260
DOEDENOEST_3894
PLAPlav_1025 Plav_1029 Plav_1034
RBSRHODOSMS8_00086(baiB)
BJAbll0131(bll0131)
BJUBJ6T_01450
BJPRN69_00685
BRABRADO6529
BBTBBta_1112
BRSS23_06710
AOLS58_10890
BRCBCCGELA001_03190
BICLMTR13_34780
BROBRAD285_6438
BRKCWS35_11230
BOTCIT37_14150
BRQCIT40_01725
BGQX265_03260
BGZXH91_04175
BSYMCIT39_00180
BBETF8237_17610
BARHWN72_46090
BVZBRAD3257_0642
BELBE61_86530
BDGLPJ38_06565
BSEPHAP48_0020605
BQBJ4P68_0013450
VGOGJW-30_1_02490(baiB_2)
XDIEZH22_21750
RBMTEF_16880
CAKCaul_3247 Caul_4604
CAULKCG34_17910
PZUPHZ_c2517
BNEDA69_06025
TSVDSM104635_02995 DSM104635_03075
YANAYJ57_19780
TMDKUV46_00545
RSUNHU_03725
RHMB5V46_17095
HNEHNE_0099(baiB)
NGFFRF71_13160
SMAZLH19_12220 LH19_27280
SPHOC3E99_13085
SWISwit_0351 Swit_1599 Swit_1600
SPHDHY78_17585 HY78_17590 HY78_17610 HY78_26355
RDICMV14_03665
SJPSJA_C2-03890 SJA_C2-04050
SCHSphch_3661
SPMIK663_20463
SPHTK426_28370
CIJWG74_11935
NAOY958_27085
CSCIHDCHBGLK_01430(baiB)
PHXKGNDJEFE_00401(baiB)
MPAMAP_2354
MITOCO_16930 OCO_48560
MIAOCU_17130 OCU_48510
MIDMIP_02338 MIP_07342
MYOOEM_14930 OEM_48710
MCHIAN480_08330 AN480_23315 AN480_25245
MIROCQ_14600 OCQ_49570
MMALCKJ54_07175 CKJ54_23135
MMANMMAN_24730 MMAN_36630
MSAMycsm_00180
MULMUL_3844
MMCMmcs_0223
MKMMkms_0233
MJLMjls_0213
MMIMMAR_3983
MMAEMMARE11_37900
MMMW7S_07140 W7S_24315
MLIMULP_04163
MKNMKAN_05880
MYEAB431_01945
MGOAFA91_08425
MSHGMSG_00422 MSG_03568
MFJMFLOJ_30860 MFLOJ_51280
MSTOMSTO_34790 MSTO_55390
MSIMMSIM_20450 MSIM_40770
MSAKMSAS_18290
MKUI2456_02760 I2456_18740
MXEMYXE_17320 MYXE_46350
MNVMNVI_02600 MNVI_19750
MPAGC0J29_02725 C0J29_21705
MGORH0P51_02740 H0P51_19675
MCOOMCOO_16060 MCOO_35900
MBAIMB901379_03460(lcfB_9)
MSEOMSEO_23560 MSEO_43170
MHEKJMUB5695_01801 JMUB5695_04479
MGAUMGALJ_47310
MLJMLAC_10490
MBRDMBRA_12830 MBRA_32660
MMAMK3U93_16925
MHOLK3U96_25350
MSENK3U95_01455
MPAEK0O64_01570
MSPGF6B93_16025
MOTLTS72_05340
MRFMJO55_01200
MPAAMKK62_07880 MKK62_23045
MCROMI149_02120
MSMMSMEG_0318
MSGMSMEI_0312
MSBLJ00_01600
MSNLI99_01600
MSHLI98_01600
MVAMvan_0252
MGIMflv_0419
MCBMycch_0222
MFTXA26_02940
MPHLMPHLCCUG_04868(baiB_2)
MVQMYVA_0257
MLLB1R94_01565
MRHMycrhN_1496
MTHN4412656_00248(fadD_2)
MDUMDUV_11370
MCHTMCHIJ_07000
MAUUNCTC10437_00256
MMORMMOR_01890 MMOR_58370
MAICMAIC_53080
MIJMINS_27090
MTYMTOK_10360
MPSCMPSYJ_06360
MGADMGAD_45630
MHEVMHEL_18710
MSARMSAR_18910
MANYMANY_43890
MPOFMPOR_07960
MBOKMBOE_15230
MFGK6L26_30000
MFLVNCTC10271_04880
MCEEMCEL_29920
MABMAB_2316
MMVMYCMA_1247
MABBMASS_2242
MABLMMASJCM_2266
MCHEBB28_11610
MIZBAB75_12360
MSTEMSTE_02250
MSAOMYCSP_11375
MSALDSM43276_02035(baiB)
CGYCGLY_15810
NBRO3I_021420
NNONONO_c50630
NOZDMB37_08020
NYALTV02_22920
RTMG4H71_11130
RKOJWS14_47605
GOMD7316_03708(baiB_2)
SBHSBI_00137
STRCAA958_12300
SNZDC008_01285
SSEOD0Z67_25560
SBATG4Z16_17215
YIALO772_03550 LO772_29980 LO772_35175
CPHAFGI33_11885
RFSC1I64_10215
CARTPA27867_2220
BRRC1N80_03880
AERAERYTH_14445
ACTWF7P10_09660 F7P10_11280
NOWGBF35_30420
FREFranean1_0614 Franean1_4026
FRIFraEuI1c_2666 FraEuI1c_3335 FraEuI1c_4447 FraEuI1c_5318 FraEuI1c_5444
SENSACE_3387(baiB)
AMYYYIM_33605(baiB2)
PDXPsed_0542
PSEAWY02_09705
PSEEFRP1_04515
PSEHXF36_02965
PSEQAD006_12175
PECQAD017_20000
PAUTPdca_52450
PBROHOP40_02455
SSYIEKG83_31105
KALKALB_5626
KPHYAOZ06_30715
MICHFJK98_11545
PLKCIK06_12190
VERHUT12_13430
EKEEK0264_04430
AYMYM304_25790
ATQGH723_15305
AQZKSP35_22785
ELEElen_3099
 » show all
Reference
1  [PMID:13303991]
  Authors
ELLIOTT WH.
  Title
The enzymic activation of cholic acid by guinea-pig-liver microsomes.
  Journal
Biochem J 62:427-33 (1956)
DOI:10.1042/bj0620427
Reference
2  [PMID:13403911]
  Authors
ELLIOTT WH.
  Title
The breakdown of adenosine triphosphate accompanying cholic acid activation by guinea-pig liver microsomes.
  Journal
Biochem J 65:315-21 (1957)
DOI:10.1042/bj0650315
Reference
3  [PMID:3183523]
  Authors
Prydz K, Kase BF, Bjorkhem I, Pedersen JI.
  Title
Subcellular localization of 3 alpha, 7 alpha-dihydroxy- and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-coenzyme A ligase(s) in rat liver.
  Journal
J Lipid Res 29:997-1004 (1988)
Reference
4  [PMID:2521999]
  Authors
Schepers L, Casteels M, Verheyden K, Parmentier G, Asselberghs S, Eyssen HJ, Mannaerts GP.
  Title
Subcellular distribution and characteristics of trihydroxycoprostanoyl-CoA synthetase in rat liver.
  Journal
Biochem J 257:221-9 (1989)
DOI:10.1042/bj2570221
Reference
5  [PMID:1551828]
  Authors
Mallonee DH, Adams JL, Hylemon PB
  Title
The bile acid-inducible baiB gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A ligase.
  Journal
J Bacteriol 174:2065-71 (1992)
DOI:10.1128/JB.174.7.2065-2071.1992
  Sequence
Reference
6  [PMID:9390170]
  Authors
Wheeler JB, Shaw DR, Barnes S.
  Title
Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes.
  Journal
Arch Biochem Biophys 348:15-24 (1997)
DOI:10.1006/abbi.1997.0391
  Sequence
[rno:79111]
Reference
7  [PMID:12454267]
  Authors
Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S.
  Title
Molecular cloning and expression of rat liver bile acid CoA ligase.
  Journal
J Lipid Res 43:2062-71 (2002)
DOI:10.1194/jlr.m200260-jlr200
  Sequence
[rno:79111]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.7
IUBMB Enzyme Nomenclature: 6.2.1.7
ExPASy - ENZYME nomenclature database: 6.2.1.7
BRENDA, the Enzyme Database: 6.2.1.7
CAS: 9027-90-1

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