KEGG   ENZYME: 6.3.2.26
Entry
EC 6.3.2.26                 Enzyme                                 
Name
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase;
L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine synthetase;
ACV synthetase;
L-alpha-aminoadipyl-cysteinyl-valine synthetase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
L-2-aminohexanedioate:L-cysteine:L-valine ligase (AMP-forming, valine-inverting)
Reaction(IUBMB)
3 ATP + L-2-aminohexanedioate + L-cysteine + L-valine + H2O = 3 AMP + 3 diphosphate + N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [RN:R04870]
Reaction(KEGG)
R04870
Substrate
ATP [CPD:C00002];
L-2-aminohexanedioate [CPD:C00956];
L-cysteine [CPD:C00097];
L-valine [CPD:C00183];
H2O [CPD:C00001]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [CPD:C05556]
Comment
Requires Mg2+. The enzyme contains 4'-phosphopantetheine, which may be involved in the mechanism of the reaction. Forms part of the penicillin biosynthesis pathway (for pathway, click here).
History
EC 6.3.2.26 created 2002
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12743  N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase
Genes
ANIAN2621.2
AORAO090038000543
AFVAFLA_009029
APUUAPUU_12364S
ABEARB_02226
TVETRV_00553
SCTSCAT_5684(pcbAB)
SCYSCATT_56840
SCLFBB341_07790
STIRDDW44_23095
AABA4R43_03220
AMYYYIM_39185(srfAA)
PSUUPsuf_059700
 » show all
Reference
1  [PMID:11851475]
  Authors
Byford MF, Baldwin JE, Shiau CY, Schofield CJ.
  Title
The Mechanism of ACV Synthetase.
  Journal
Chem Rev 97:2631-2650 (1997)
DOI:10.1021/cr960018l
Reference
2  [PMID:9355751]
  Authors
Theilgaard HB, Kristiansen KN, Henriksen CM, Nielsen J.
  Title
Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum.
  Journal
Biochem J 327 ( Pt 1):185-91 (1997)
DOI:10.1042/bj3270185
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.26
IUBMB Enzyme Nomenclature: 6.3.2.26
ExPASy - ENZYME nomenclature database: 6.3.2.26
BRENDA, the Enzyme Database: 6.3.2.26
CAS: 57219-73-5

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