KEGG   ENZYME: 6.4.1.8
Entry
EC 6.4.1.8                  Enzyme                                 
Name
acetophenone carboxylase
Class
Ligases;
Forming carbon-carbon bonds;
Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
Sysname
acetophenone:carbon-dioxide ligase (ADP-forming)
Reaction(IUBMB)
2 ATP + acetophenone + HCO3- + H2O + H+ = 2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate [RN:R05453]
Reaction(KEGG)
R05453
Substrate
ATP [CPD:C00002];
acetophenone [CPD:C07113];
HCO3- [CPD:C00288];
H2O [CPD:C00001];
H+ [CPD:C00080]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
3-oxo-3-phenylpropanoate [CPD:C07114]
Comment
The enzyme is involved in anaerobic degradation of ethylbenzene. No activity with acetone, butanone, 4-hydroxy-acetophenone or 4-amino-acetophenone.
History
EC 6.4.1.8 created 2011
Pathway
ec00642  Ethylbenzene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K10701  acetophenone carboxylase
Genes
EBAc1A100(apc4) c1A102(apc3) c1A105(apc1) c1A200(apc2) c1A97(apc5)
ABREpbN1_00090(apcE) pbN1_00120(apcB)
DDENKI615_16780 KI615_16790
RMARGBA65_17305 GBA65_17310
HAKZJ0X25_19550 J0X25_19560 J0X25_19565
Reference
1  [PMID:20047908]
  Authors
Jobst B, Schuhle K, Linne U, Heider J
  Title
ATP-dependent carboxylation of acetophenone by a novel type of carboxylase.
  Journal
J Bacteriol 192:1387-94 (2010)
DOI:10.1128/JB.01423-09
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.4.1.8
IUBMB Enzyme Nomenclature: 6.4.1.8
ExPASy - ENZYME nomenclature database: 6.4.1.8
UM-BBD (Biocatalysis/Biodegradation Database): 6.4.1.8
BRENDA, the Enzyme Database: 6.4.1.8

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