KEGG   ENZYME: 7.2.4.5
Entry
EC 7.2.4.5                  Enzyme                                 

Name
glutaconyl-CoA decarboxylase;
glutaconyl coenzyme A decarboxylase;
pent-2-enoyl-CoA carboxy-lyase;
4-carboxybut-2-enoyl-CoA carboxy-lyase
Class
Translocases;
Catalysing the translocation of inorganic cations;
Linked to decarboxylation
Sysname
(2E)-4-carboxybut-2-enoyl-CoA carboxy-lyase [(2E)-but-2-enoyl-CoA-forming]
Reaction(IUBMB)
(2E)-4-carboxybut-2-enoyl-CoA + Na+[side 1] = (2E)-but-2-enoyl-CoA + CO2 + Na+[side 2] [RN:R03028]
Reaction(KEGG)
R03028
Substrate
(2E)-4-carboxybut-2-enoyl-CoA [CPD:C02411];
Na+[side 1]
Product
(2E)-but-2-enoyl-CoA [CPD:C00877];
CO2 [CPD:C00011];
Na+[side 2]
Comment
The enzyme from the bacterium Acidaminococcus fermentans is a biotinyl-protein, requires Na+, and acts as a sodium pump. Prior to the Na+-dependent decarboxylation, the carboxylate is transferred to biotin in a Na+-independent manner. The conserved lysine, to which biotin forms an amide bond, is located 34 amino acids before the C-terminus, flanked on both sides by two methionine residues, which are conserved in every biotin-dependent enzyme.
History
EC 7.2.4.5 created 1986 as EC 4.1.1.70, modified 2003, transferred 2019 to EC 7.2.4.5
Pathway
ec00362  Benzoate degradation
ec00650  Butanoate metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K01615  glutaconyl-CoA decarboxylase subunit alpha
Genes
GEO: Geob_0238(gcdA)
GEM: GM21_2800
GBM: Gbem_1453(gcdA)
GBN: GEOBRER4_29690
DML: Dmul_25060(gcdA)
DAT: HRM2_38510(gcdA1) HRM2_38640(gcdA2)
DTO: TOL2_C38230
DOV: DSCO28_25370 DSCO28_61390
DWD: DSCW_22800
DALK: DSCA_21110 DSCA_57350
DLI: dnl_11250(gcdA)
DMM: dnm_079260(gcdA)
SAT: SYN_00481
DBR: Deba_2446
OVA: OBV_10900
CSO: CLS_16270
FAA: HMPREF0389_01471(gcdA)
BPRS: CK3_23950
PED: ING2D1G_1422(gcdA)
PHAR: NCTC13077_00176(gcdA)
AIN: Acin_0318
FNU: FN0204
 » show all
Reference
1  [PMID:6628393]
  Authors
Buckel W, Semmler R.
  Title
Purification, characterisation and reconstitution of glutaconyl-CoA decarboxylase, a biotin-dependent sodium pump from anaerobic bacteria.
  Journal
Eur J Biochem 136:427-34 (1983)
DOI:10.1111/j.1432-1033.1983.tb07760.x
Reference
2  [PMID:11248185]
  Authors
Buckel W.
  Title
Sodium ion-translocating decarboxylases.
  Journal
Biochim Biophys Acta 1505:15-27 (2001)
DOI:10.1016/S0005-2728(00)00273-5
Other DBs
ExplorEnz - The Enzyme Database: 7.2.4.5
IUBMB Enzyme Nomenclature: 7.2.4.5
ExPASy - ENZYME nomenclature database: 7.2.4.5
BRENDA, the Enzyme Database: 7.2.4.5
CAS: 84399-93-9

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