KEGG   ORTHOLOGY: K00492
Entry
K00492            Tight     KO                                     

Name
tmuM
Definition
1,3,7-trimethyluric acid 5-monooxygenase [EC:1.14.13.212]
Pathway
ko00232  Caffeine metabolism
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00232 Caffeine metabolism
    K00492  tmuM; 1,3,7-trimethyluric acid 5-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.212  1,3,7-trimethyluric acid 5-monooxygenase
     K00492  tmuM; 1,3,7-trimethyluric acid 5-monooxygenase
Other DBs
RN: R11153
Genes
AG: AFO10115(tmuM)
Reference
  Authors
Mohanty SK, Yu CL, Das S, Louie TM, Gakhar L, Subramanian M
  Title
Delineation of the caffeine C-8 oxidation pathway in Pseudomonas sp. strain CBB1 via characterization of a new trimethyluric acid monooxygenase and genes involved in trimethyluric acid metabolism.
  Journal
J Bacteriol 194:3872-82 (2012)
DOI:10.1128/JB.00597-12
  Sequence

KEGG   ENZYME: 1.14.13.212
Entry
EC 1.14.13.212              Enzyme                                 

Name
1,3,7-trimethyluric acid 5-monooxygenase;
tmuM (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
1,3,7-trimethylurate,NADH:oxygen oxidoreductase (1,3,7-trimethyl-5-hydroxyisourate forming)
Reaction(IUBMB)
1,3,7-trimethylurate + NADH + H+ + O2 = 1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O [RN:R11153]
Reaction(KEGG)
R11153
Substrate
1,3,7-trimethylurate [CPD:C16361];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
1,3,7-trimethyl-5-hydroxyisourate [CPD:C21144];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC 1.14.13.113, FAD-dependent urate hydroxylase.
History
EC 1.14.13.212 created 2016
Pathway
ec00232  Caffeine metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K00492  1,3,7-trimethyluric acid 5-monooxygenase
Reference
1  [PMID:22609920]
  Authors
Mohanty SK, Yu CL, Das S, Louie TM, Gakhar L, Subramanian M
  Title
Delineation of the caffeine C-8 oxidation pathway in Pseudomonas sp. strain CBB1 via characterization of a new trimethyluric acid monooxygenase and genes involved in trimethyluric acid metabolism.
  Journal
J Bacteriol 194:3872-82 (2012)
DOI:10.1128/JB.00597-12
  Sequence
Reference
2  [PMID:25678373]
  Authors
Summers RM, Mohanty SK, Gopishetty S, Subramanian M
  Title
Genetic characterization of caffeine degradation by bacteria and its potential applications.
  Journal
Microb Biotechnol 8:369-78 (2015)
DOI:10.1111/1751-7915.12262
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.212
IUBMB Enzyme Nomenclature: 1.14.13.212
ExPASy - ENZYME nomenclature database: 1.14.13.212
BRENDA, the Enzyme Database: 1.14.13.212

KEGG   REACTION: R11153
Entry
R11153                      Reaction                               

Name
1,3,7-trimethylurate,NADH:oxygen oxidoreductase (1,3,7-trimethyl-5-hydroxyisourate forming)
Definition
1,3,7-Trimethyluric acid + NADH + H+ + Oxygen <=> 1,3,7-Trimethyl-5-hydroxyisourate + NAD+ + H2O
Equation
Reaction class
RC00001  C00003_C00004
RC03373  C16361_C21144
Enzyme
Pathway
rn00232  Caffeine metabolism
rn01120  Microbial metabolism in diverse environments
Orthology
K00492  1,3,7-trimethyluric acid 5-monooxygenase [EC:1.14.13.212]
Other DBs
RHEA: 48995

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