KEGG   ORTHOLOGY: K00607
Entry
K00607                      KO                                     
Symbol
wbkC
Name
GDP-perosamine N-formyltransferase [EC:2.1.2.14]
Pathway
map00541  O-Antigen nucleotide sugar biosynthesis
map01100  Metabolic pathways
map01250  Biosynthesis of nucleotide sugars
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09107 Glycan biosynthesis and metabolism
   00541 O-Antigen nucleotide sugar biosynthesis
    K00607  wbkC; GDP-perosamine N-formyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.2  Hydroxymethyl-, formyl- and related transferases
    2.1.2.14  GDP-perosamine N-formyltransferase
     K00607  wbkC; GDP-perosamine N-formyltransferase
Other DBs
RN: R12790
COG: COG0223
Genes
CNV: CNZW441b_1265
BME: BMEI1418
BMEL: DK63_2070
BMI: BMEA_A0555
BMZ: BM28_A0534
BMG: BM590_A0536
BMW: BMNI_I0525
BMEE: DK62_884
BMF: BAB1_0540
BMB: BruAb1_0540
BABO: DK55_547
BABR: DO74_1339
BABT: DK49_311
BABB: DK48_1578
BABU: DK53_537
BABS: DK51_923
BABC: DO78_454
BMS: BR0517
BMT: BSUIS_A0548(arnA)
BSZ: DK67_1446
BOV: BOV_0521
BCS: BCAN_A0529(arnA)
BCAR: DK60_600
BCAS: DA85_02475
BMR: BMI_I519
BPP: BPI_I548
BPV: DK65_842
 » show all
Reference
  Authors
Godfroid F, Cloeckaert A, Taminiau B, Danese I, Tibor A, de Bolle X, Mertens P, Letesson JJ.
  Title
Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of brucella melitensis 16M (wbk).
  Journal
Res Microbiol 151:655-68 (2000)
DOI:10.1016/s0923-2508(00)90130-x
  Sequence
[bme:BMEI1418]

KEGG   ENZYME: 2.1.2.14
Entry
EC 2.1.2.14                 Enzyme                                 
Name
GDP-perosamine N-formyltransferase;
wbkC (gene name)
Class
Transferases;
Transferring one-carbon groups;
Hydroxymethyl-, formyl- and related transferases
Sysname
10-formyltetrahydrofolate:GDP-alpha-D-perosamine N-formyltransferase
Reaction(IUBMB)
10-formyltetrahydrofolate + GDP-alpha-D-perosamine = tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine [RN:R12790]
Reaction(KEGG)
R12790
Substrate
10-formyltetrahydrofolate [CPD:C00234];
GDP-alpha-D-perosamine [CPD:C20638]
Product
tetrahydrofolate [CPD:C00101];
GDP-N-formyl-alpha-D-perosamine [CPD:C22397]
Comment
The enzyme, characterized from the bacterium Brucella melitensis, synthesizes a building block of the O antigen produced by Brucella species.
History
EC 2.1.2.14 created 2021
Pathway
ec00541  O-Antigen nucleotide sugar biosynthesis
ec01100  Metabolic pathways
Orthology
K00607  GDP-perosamine N-formyltransferase
Genes
CNVCNZW441b_1265
BMEBMEI1418
BMELDK63_2070
BMIBMEA_A0555
BMZBM28_A0534
BMGBM590_A0536
BMWBMNI_I0525
BMEEDK62_884
BMFBAB1_0540
BMBBruAb1_0540
BMCBAbS19_I05060
BAABAA13334_I03106
BABODK55_547
BABRDO74_1339
BABTDK49_311
BABBDK48_1578
BABUDK53_537
BABSDK51_923
BABCDO78_454
BMSBR0517
BSIBS1330_I0516
BSFBSS2_I0504
BSUIBSSP1_I1186
BSUPBSPT1_I1197
BSUVBSPT2_I1182
BSUCBSSP2_I0533
BMTBSUIS_A0548(arnA)
BSZDK67_1446
BSVBSVBI22_A0516
BSWIY71_02635
BSGIY72_02360
BOVBOV_0521
BCSBCAN_A0529(arnA)
BSKBCA52141_I0862
BOLBCOUA_I0517
BCARDK60_600
BCASDA85_02475
BMRBMI_I519
BPPBPI_I548
BPVDK65_842
BVLBF3285c1_0240
BRUBFS01_02560
 » show all
Reference
1  [PMID:11081580]
  Authors
Godfroid F, Cloeckaert A, Taminiau B, Danese I, Tibor A, de Bolle X, Mertens P, Letesson JJ.
  Title
Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of brucella melitensis 16M (wbk).
  Journal
Res Microbiol 151:655-68 (2000)
DOI:10.1016/s0923-2508(00)90130-x
  Sequence
[bme:BMEI1418]
Reference
2  [PMID:28636341]
  Authors
Riegert AS, Chantigian DP, Thoden JB, Tipton PA, Holden HM.
  Title
Biochemical Characterization of WbkC, an N-Formyltransferase from Brucella melitensis.
  Journal
Biochemistry 56:3657-3668 (2017)
DOI:10.1021/acs.biochem.7b00494
  Sequence
[bme:BMEI1418]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.2.14
IUBMB Enzyme Nomenclature: 2.1.2.14
ExPASy - ENZYME nomenclature database: 2.1.2.14
BRENDA, the Enzyme Database: 2.1.2.14

KEGG   REACTION: R12790
Entry
R12790                      Reaction                               
Name
10-formyltetrahydrofolate:GDP-alpha-D-perosamine N-formyltransferase
Definition
10-Formyltetrahydrofolate + GDP-4-amino-4,6-dideoxy-alpha-D-mannose <=> Tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
Equation
Reaction class
RC00026  C00101_C00234
Enzyme
Pathway
rn00541  O-Antigen nucleotide sugar biosynthesis
rn01100  Metabolic pathways
rn01250  Biosynthesis of nucleotide sugars
Orthology
K00607  GDP-perosamine N-formyltransferase [EC:2.1.2.14]
Other DBs
RHEA: 68591

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