Requires Mg2+ for activity. While purified enzyme from Escherichia coli is specific for acetate , others have found that the enzyme can also use propanoate as a substrate, but more slowly . Acetate can be converted into the key metabolic intermediate acetyl-CoA by coupling acetate kinase with EC 18.104.22.168
, phosphate acetyltransferase. Both this enzyme and EC 22.214.171.124
, propionate kinase, play important roles in the production of propanoate .