KEGG ORTHOLOGY: K01453

ORTHOLOGY: K01453

Entry

K01453                      KO

Symbol

nylB

Name

6-aminohexanoate-oligomer exohydrolase [EC:3.5.1.46]

Pathway

map00930

Caprolactam degradation

map01120

Microbial metabolism in diverse environments

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00930 Caprolactam degradation
    K01453  nylB; 6-aminohexanoate-oligomer exohydrolase
Enzymes [BR:ko01000]
3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.1  In linear amides
    3.5.1.46  6-aminohexanoate-oligomer exohydrolase
     K01453  nylB; 6-aminohexanoate-oligomer exohydrolase

Other DBs

RN:	R00059 R10975 R10979

GO:

0019875

Genes

RPEL:

N7U68_18585

SLON:

LGI35_36615

MNF:	JSY13_10210

AMAU:

DSM26151_03960(nylB')

LEU:	Leucomu_04960

LTR:	EVS81_02965

LLUT:

K1X41_10430

ARR:	ARUE_c40930(nylB)

AAU:	AAur_3953(nylB)

NDK:	I601_2859(nylB')

AG:	BAA05087(nylB) BAA05089(nylB')

» show all

Reference

PMID:11092619

Authors

Negoro S

Title

Biodegradation of nylon oligomers.

Journal

Appl Microbiol Biotechnol 54:461-6 (2000)
DOI:10.1007/s002530000434

Sequence

[ag:BAA05087 BAA05089]

Reference

PMID:6646204

Authors

Okada H, Negoro S, Kimura H, Nakamura S

Title

Evolutionary adaptation of plasmid-encoded enzymes for degrading nylon oligomers.

Journal

Nature 306:203-6 (1983)
DOI:10.1038/306203a0

Sequence

[ag:BAA05087 BAA05089]

All links

Ontology (3)   
   KEGG BRITE (2)   
   GO (1)   
Pathway (4)   
   KEGG PATHWAY (4)   
Chemical reaction (6)   
   KEGG ENZYME (1)   
   KEGG REACTION (3)   
   KEGG RCLASS (2)   
Gene (13)   
   KEGG GENES (12)   
   OC (1)   
Literature (2)   
   PubMed (2)   
All databases (28)   

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ENZYME: 3.5.1.46

Entry

EC 3.5.1.46                 Enzyme

Name

6-aminohexanoate-oligomer exohydrolase;
6-aminohexanoate-dimer hydrolase;
nylB (gene name);
6-aminohexanoic acid oligomer hydrolase (ambiguous);
N-(6-aminohexanoyl)-6-aminohexanoate amidohydrolase;
nylon-6 hydrolase (ambiguous)

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

Sysname

N-(6-aminohexanoyl)-6-aminohexanoate exoamidohydrolase

Reaction(IUBMB)

(1) [N-(6-aminohexanoyl)]n + H2O = [N-(6-aminohexanoyl)]n-1 + 6-aminohexanoate [RN:R10975];
(2) N-(6-aminohexanoyl)-6-aminohexanoate + H2O = 2 6-aminohexanoate [RN:R00059]

Reaction(KEGG)

R00059 R10975;
(other) R10979

Substrate

[N-(6-aminohexanoyl)]n [CPD:C20988];
H2O [CPD:C00001];
N-(6-aminohexanoyl)-6-aminohexanoate [CPD:C01255]

Product

[N-(6-aminohexanoyl)]n-1;
6-aminohexanoate [CPD:C02378]

Comment

The enzyme is involved in degradation of nylon-6 oligomers. It degrades linear oligomers of 6-aminohexanoate with a degree of polymerization of 2--20 by exo-type cleavage, removing residues sequentially from the N-terminus. Activity decreases with the increase of the polymerization number of the oligomer. cf. EC 3.5.1.117, 6-aminohexanoate-oligomer endohydrolase and EC 3.5.2.12, 6-aminohexanoate-cyclic-dimer hydrolase.

History

EC 3.5.1.46 created 1983, modified 2014

Pathway

ec00930

Caprolactam degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K01453

6-aminohexanoate-oligomer exohydrolase

Genes

RPEL:

N7U68_18585

SLON:

LGI35_36615

MNF:	JSY13_10210

AMAU:

DSM26151_03960(nylB')

LEU:	Leucomu_04960

LTR:	EVS81_02965

LLUT:

K1X41_10430

ARR:	ARUE_c40930(nylB)

AAU:	AAur_3953(nylB)

NDK:	I601_2859(nylB')

Reference

1 [PMID:7262074]

Authors

Kinoshita S, Terada T, Taniguchi T, Takene Y, Masuda S, Matsunaga N, Okada H.

Title

Purification and characterization of 6-aminohexanoic-acid-oligomer hydrolase of Flavobacterium sp. Ki72.

Journal

Eur J Biochem 116:547-51 (1981)
DOI:10.1111/j.1432-1033.1981.tb05371.x

Other DBs

ExplorEnz - The Enzyme Database:

3.5.1.46

IUBMB Enzyme Nomenclature:

3.5.1.46

ExPASy - ENZYME nomenclature database:

3.5.1.46

UM-BBD (Biocatalysis/Biodegradation Database):

3.5.1.46

BRENDA, the Enzyme Database:

3.5.1.46

CAS:	75216-15-8

REACTION: R00059

Entry

R00059                      Reaction

Name

N-(6-aminohexanoyl)-6-aminohexanoate amidohydrolase

Definition

N-(6-Aminohexanoyl)-6-aminohexanoate + H2O <=> 2 6-Aminohexanoate

Equation

C01255 + C00001 <=> 2 C02378

Reaction class

RC00090

C01255_C02378

RC00096

C01255_C02378

Enzyme

3.5.1.46

Pathway

rn00930

Caprolactam degradation

rn01120

Microbial metabolism in diverse environments

Orthology

K01453

6-aminohexanoate-oligomer exohydrolase [EC:3.5.1.46]

Other DBs

RHEA:

21367

All links

Ontology (1)   
   KEGG BRITE (1)   
Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (3)   
   KEGG COMPOUND (3)   
Chemical reaction (4)   
   KEGG ENZYME (1)   
   KEGG RCLASS (2)   
   UMBBD-RN (1)   
Gene (13)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (12)   
All databases (25)   

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