KEGG ORTHOLOGY: K03268

ORTHOLOGY: K03268

Entry

K03268 Tight KO

Symbol

todC1, bedC1, tcbAa

Name

benzene/toluene/chlorobenzene dioxygenase subunit alpha [EC:1.14.12.3 1.14.12.11 1.14.12.26]

Pathway

map00361

Chlorocyclohexane and chlorobenzene degradation

map00362

Benzoate degradation

map00623

Toluene degradation

map00625

Chloroalkane and chloroalkene degradation

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

map01220

Degradation of aromatic compounds

Module

M00547

Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
   00625 Chloroalkane and chloroalkene degradation
    K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
   00361 Chlorocyclohexane and chlorobenzene degradation
    K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
   00623 Toluene degradation
    K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.3  benzene 1,2-dioxygenase
     K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
    1.14.12.11  toluene dioxygenase
     K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
    1.14.12.26  chlorobenzene dioxygenase
     K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha

Other DBs

RN:	R03543 R03559 R05429 R06831 R06856 R09151 R09152

GO:	0018619 0018624 0018609

Genes

PPF:

Pput_2881

PPX:	T1E_4266(todC1)

PMON:

X969_10930

PMOT:

X970_10585

BPT:

Bpet3738

AG:	AAC43632(tcbAa)

Reference

PMID:8824602

Authors

Fong KP, Goh CB, Tan HM

Title

Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase.

Journal

J Bacteriol 178:5592-601 (1996)
DOI:10.1128/JB.178.19.5592-5601.1996

Reference

PMID:2670929

Authors

Zylstra GJ, Gibson DT

Title

Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli.

Journal

J Biol Chem 264:14940-6 (1989)

Sequence

[ppf:Pput_2881]

Reference

PMID:8626733

Authors

Werlen C, Kohler HP, van der Meer JR

Title

The broad substrate chlorobenzene dioxygenase and cis-chlorobenzene dihydrodiol dehydrogenase of Pseudomonas sp. strain P51 are linked evolutionarily to the enzymes for benzene and toluene degradation.

Journal

J Biol Chem 271:4009-16 (1996)
DOI:10.1074/jbc.271.8.4009

Sequence

[ag:AAC43632]

All links

Ontology (6)   
   KEGG BRITE (3)   
   GO (3)   
Pathway (15)   
   KEGG PATHWAY (14)   
   KEGG MODULE (1)   
Chemical reaction (16)   
   KEGG ENZYME (3)   
   KEGG REACTION (7)   
   KEGG RCLASS (6)   
Gene (7)   
   KEGG GENES (6)   
   OC (1)   
Literature (3)   
   PubMed (3)   
All databases (47)   

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ORTHOLOGY: K16268

Entry

K16268 Tight KO

Symbol

todC2, bedC2, tcbAb

Name

benzene/toluene/chlorobenzene dioxygenase subunit beta [EC:1.14.12.3 1.14.12.11 1.14.12.26]

Pathway

map00361

Chlorocyclohexane and chlorobenzene degradation

map00362

Benzoate degradation

map00623

Toluene degradation

map00625

Chloroalkane and chloroalkene degradation

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

map01220

Degradation of aromatic compounds

Module

M00547

Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
   00625 Chloroalkane and chloroalkene degradation
    K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
   00361 Chlorocyclohexane and chlorobenzene degradation
    K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
   00623 Toluene degradation
    K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.3  benzene 1,2-dioxygenase
     K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
    1.14.12.11  toluene dioxygenase
     K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
    1.14.12.26  chlorobenzene dioxygenase
     K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta

Other DBs

RN:	R03543 R03559 R05429 R06831 R06856 R09151 R09152

GO:	0018619 0018624 0018609

Genes

PPF:

Pput_2880

PPX:	T1E_4267(todC2)

PMON:

X969_10935

PMOT:

X970_10590

BPT:

Bpet3739

AG:	AAC43633(tcbAb)

Reference

PMID:8824602

Authors

Fong KP, Goh CB, Tan HM

Title

Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase.

Journal

J Bacteriol 178:5592-601 (1996)
DOI:10.1128/JB.178.19.5592-5601.1996

Reference

PMID:2670929

Authors

Zylstra GJ, Gibson DT

Title

Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli.

Journal

J Biol Chem 264:14940-6 (1989)

Sequence

[ppf:Pput_2880]

Reference

PMID:8626733

Authors

Werlen C, Kohler HP, van der Meer JR

Title

Journal

J Biol Chem 271:4009-16 (1996)
DOI:10.1074/jbc.271.8.4009

Sequence

[ag:AAC43633]

All links

Ontology (6)   
   KEGG BRITE (3)   
   GO (3)   
Pathway (15)   
   KEGG PATHWAY (14)   
   KEGG MODULE (1)   
Chemical reaction (16)   
   KEGG ENZYME (3)   
   KEGG REACTION (7)   
   KEGG RCLASS (6)   
Gene (7)   
   KEGG GENES (6)   
   OC (1)   
Literature (3)   
   PubMed (3)   
All databases (47)   

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ORTHOLOGY: K18089

Entry

K18089 Tight KO

Symbol

todB, tcbAc

Name

benzene/toluene/chlorobenzene dioxygenase ferredoxin component

Pathway

map00361

Chlorocyclohexane and chlorobenzene degradation

map00362

Benzoate degradation

map00623

Toluene degradation

map00625

Chloroalkane and chloroalkene degradation

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

map01220

Degradation of aromatic compounds

Module

M00547

Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component
   00625 Chloroalkane and chloroalkene degradation
    K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component
   00361 Chlorocyclohexane and chlorobenzene degradation
    K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component
   00623 Toluene degradation
    K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component

Other DBs

RN:	R03543 R03559 R05429 R06831 R06856 R09151 R09152

COG:

COG2146

Genes

PPF:

Pput_2879

PPX:	T1E_4268(tobB)

PMON:

X969_10940

PMOT:

X970_10595

BPT:

Bpet3740

AG:	AAC43634(tcbAc)

Reference

PMID:8626733

Authors

Werlen C, Kohler HP, van der Meer JR

Title

Journal

J Biol Chem 271:4009-16 (1996)
DOI:10.1074/jbc.271.8.4009

Sequence

[ag:AAC43634]

All links

Ontology (3)   
   KEGG BRITE (2)   
   COG (1)   
Pathway (15)   
   KEGG PATHWAY (14)   
   KEGG MODULE (1)   
Chemical reaction (13)   
   KEGG REACTION (7)   
   KEGG RCLASS (6)   
Gene (7)   
   KEGG GENES (6)   
   OC (1)   
Literature (1)   
   PubMed (1)   
All databases (39)   

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ORTHOLOGY: K18090

Entry

K18090 Tight KO

Symbol

todA, tcbAd

Name

benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component [EC:1.18.1.3 1.18.1.-]

Pathway

map00361

Chlorocyclohexane and chlorobenzene degradation

map00362

Benzoate degradation

map00623

Toluene degradation

map00625

Chloroalkane and chloroalkene degradation

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

map01220

Degradation of aromatic compounds

Module

M00547

Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
   00625 Chloroalkane and chloroalkene degradation
    K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
   00361 Chlorocyclohexane and chlorobenzene degradation
    K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
   00623 Toluene degradation
    K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.3  ferredoxin---NAD+ reductase
     K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
    1.18.1.-
     K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component

Other DBs

RN:	R03543 R03559 R05429 R06831 R06856 R09151 R09152

COG:

COG0446

GO:

0008860

Genes

PPF:

Pput_2878

PPX:	T1E_4269(tobA)

PMON:

X969_10945

PMOT:

X970_10600

BPT:

Bpet3741

AG:	AAC43635(tcbAd)

Reference

PMID:8626733

Authors

Werlen C, Kohler HP, van der Meer JR

Title

Journal

J Biol Chem 271:4009-16 (1996)
DOI:10.1074/jbc.271.8.4009

Sequence

[ag:AAC43635]

All links

Ontology (5)   
   KEGG BRITE (3)   
   GO (1)   
   COG (1)   
Pathway (15)   
   KEGG PATHWAY (14)   
   KEGG MODULE (1)   
Chemical reaction (14)   
   KEGG ENZYME (1)   
   KEGG REACTION (7)   
   KEGG RCLASS (6)   
Gene (7)   
   KEGG GENES (6)   
   OC (1)   
Literature (1)   
   PubMed (1)   
All databases (42)   

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ENZYME: 1.14.12.11

Entry

EC 1.14.12.11 Enzyme

Name

toluene dioxygenase;
toluene 2,3-dioxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating)

Reaction(IUBMB)

toluene + NADH + H+ + O2 = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R03559]

Reaction(KEGG)

R03559;
(other) R09151 R09152

Substrate

toluene [CPD:C01455];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol [CPD:C04592];
NAD+ [CPD:C00003]

Comment

A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase, and a ferredoxin. Some other aromatic compounds, including ethylbenzene, 4-xylene and some halogenated toluenes, are converted into the corresponding cis-dihydrodiols.

History

EC 1.14.12.11 created 1992

Pathway

ec00623

Toluene degradation

ec00625

Chloroalkane and chloroalkene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K03268

benzene/toluene/chlorobenzene dioxygenase subunit alpha

K16268

benzene/toluene/chlorobenzene dioxygenase subunit beta

Genes

PPF:	Pput_2880 Pput_2881

PPX:	T1E_4266(todC1) T1E_4267(todC2)

PMON:

X969_10930 X969_10935

PMOT:

X970_10585 X970_10590

BPT:	Bpet3738 Bpet3739

Reference

1 [PMID:16347845]

Authors

Renganathan V.

Title

Possible Involvement of Toluene-2,3-Dioxygenase in Defluorination of 3-Fluoro-Substituted Benzenes by Toluene-Degrading Pseudomonas sp. Strain T-12.

Journal

Appl Environ Microbiol 55:330-334 (1989)
DOI:10.1128/AEM.55.2.330-334.1989

Reference

2 [PMID:526270]

Authors

Subramanian V, Liu TN, Yeh WK, Gibson DT.

Title

Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography.

Journal

Biochem Biophys Res Commun 91:1131-9 (1979)
DOI:10.1016/0006-291X(79)91998-3

Other DBs

ExplorEnz - The Enzyme Database:

1.14.12.11

IUBMB Enzyme Nomenclature:

1.14.12.11

ExPASy - ENZYME nomenclature database:

1.14.12.11

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.12.11

BRENDA, the Enzyme Database:

1.14.12.11

CAS:	120038-36-0

REACTION: R09151

Entry

R09151 Reaction

Name

trichloroethene,NADH:oxygen oxidoreductase (glyoxylate-forming)

Definition

Trichloroethene + Oxygen + H2O + NADH + H+ <=> Glyoxylate + 3 Hydrochloric acid + NAD+

Equation

C06790 + C00007 + C00001 + C00004 + C00080 <=> C00048 + 3 C01327 + C00003

Comment

dioxygenase (distinct from R09141+R09144)
requiring ferredoxin and ferredoxin reductase (ec 1.18.1.3)

Reaction class

RC00001

C00003_C00004

RC03011

C00048_C06790

Enzyme

1.14.12.11

Pathway

rn00625

Chloroalkane and chloroalkene degradation

Orthology

K03268

benzene/toluene/chlorobenzene dioxygenase subunit alpha [EC:1.14.12.3 1.14.12.11 1.14.12.26]

K16268

benzene/toluene/chlorobenzene dioxygenase subunit beta [EC:1.14.12.3 1.14.12.11 1.14.12.26]

K18089

benzene/toluene/chlorobenzene dioxygenase ferredoxin component

K18090

benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component [EC:1.18.1.3 1.18.1.-]

All links

Ontology (1)   
   KEGG BRITE (1)   
Pathway (2)   
   KEGG PATHWAY (2)   
Chemical substance (8)   
   KEGG COMPOUND (8)   
Chemical reaction (4)   
   KEGG ENZYME (1)   
   KEGG RCLASS (2)   
   UMBBD-RN (1)   
Gene (28)   
   KEGG ORTHOLOGY (4)   
   KEGG GENES (24)   
All databases (43)   

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