KEGG   ORTHOLOGY: K03383
Entry
K03383                      KO                                     
Symbol
atzD
Name
cyanuric acid amidohydrolase [EC:3.5.2.15]
Pathway
map00791  Atrazine degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00791 Atrazine degradation
    K03383  atzD; cyanuric acid amidohydrolase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.2  In cyclic amides
    3.5.2.15  cyanuric acid amidohydrolase
     K03383  atzD; cyanuric acid amidohydrolase
Other DBs
RN: R12332
GO: 0018753
Genes
PPSE: BN5_3198(trzD)
HMI: soil367_16665
NTG: NSCAC_1002
ADI: B5T_00742
AXE: P40_03495
ACII: C4901_13330
ATY: A9R16_003775
AANT: HUK68_18800
VPD: VAPA_1c50370
VAA: AX767_09230
HYB: Q5W_10340
AVI: Avi_5653
RLE: pRL100353(atzD)
RLG: Rleg_6452
RHL: LPU83_pLPU83c0721(atzD)
RII: FFM53_035660(atzD)
RRG: J3P73_33550(atzD)
BJA: blr7281(atzD)
BRA: BRADO6319(atzD)
BBT: BBta_1315(atzD)
BRS: S23_59060(atzD)
AOL: S58_64660
BRAD: BF49_2819
BRO: BRAD285_6240(atzD)
BARH: WN72_41590
BVZ: BRAD3257_1357(atzD)
MET: M446_3816
MOR: MOC_0480
MIND: mvi_43360
PLEO: OHA_1_04335(trzD)
HDI: HDIA_3388(trzD)
CID: P73_3150
AFR: AFE_0779
ACU: Atc_m024
SAY: TPY_1352
MTA: Moth_2120
MTHO: MOTHE_c21730(trzD)
MTHZ: MOTHA_c22470(trzD)
NWL: NWFMUON74_55550(atzD)
GOR: KTR9_4373
PDX: Psed_3188
AG: AAC61577(trzD)
 » show all
Reference
PMID:1991731
  Authors
Eaton RW, Karns JS
  Title
Cloning and comparison of the DNA encoding ammelide aminohydrolase and cyanuric acid amidohydrolase from three s-triazine-degrading bacterial strains.
  Journal
J Bacteriol 173:1363-6 (1991)
DOI:10.1128/JB.173.3.1363-1366.1991
  Sequence

KEGG   ENZYME: 3.5.2.15
Entry
EC 3.5.2.15                 Enzyme                                 
Name
cyanuric acid amidohydrolase;
atzD (gene name);
trzD (gene name)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides
Sysname
cyanuric acid amidohydrolase
Reaction(IUBMB)
cyanuric acid + H2O = 1-carboxybiuret [RN:R12332]
Reaction(KEGG)
R12332
Substrate
cyanuric acid [CPD:C06554];
H2O [CPD:C00001]
Product
1-carboxybiuret [CPD:C22084]
Comment
The enzyme catalyses the ring cleavage of cyanuric acid, an intermediate in the degradation of s-triazide herbicides such as atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine]. The enzyme is highly specific for cyanuric acid. The product was initially thought to be bioret, but was later shown to be 1-carboxybioret.
History
EC 3.5.2.15 created 2000, modified 2008, modified 2019
Pathway
ec00791  Atrazine degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03383  cyanuric acid amidohydrolase
Genes
PPSEBN5_3198(trzD)
HMIsoil367_16665
NTGNSCAC_1002
ADIB5T_00742
AXEP40_03495
ACIIC4901_13330
ATYA9R16_003775
AANTHUK68_18800
VPDVAPA_1c50370
VAAAX767_09230
CSERCCO03_17450
HYBQ5W_10340
ATERMW290_21240
RGUA4W93_01790
AVIAvi_5653
ARUIG6M88_15985
RLEpRL100353(atzD)
RLGRleg_6452
RLBRLEG3_02325
RTRRTCIAT899_PC08525
RHLLPU83_pLPU83c0721(atzD)
RHRCKA34_30155
RGRFZ934_06705
RIIFFM53_035660(atzD)
RRGJ3P73_33550(atzD)
RLSHB780_09660
NGLRG1141_CH21690
NGGRG540_CH22560
BJAblr7281(atzD)
BJUBJ6T_21060
BJPRN69_10315
BRABRADO6319(atzD)
BBTBBta_1315(atzD)
BRSS23_59060(atzD)
AOLS58_64660
BRCBCCGELA001_07285
BRADBF49_2819
BROBRAD285_6240(atzD)
BRQCIT40_04950
BGQX265_30660
BGZXH91_29120
BSYMCIT39_03550
BBETF8237_09205
BARHWN72_41590
BVZBRAD3257_1357(atzD)
BDGLPJ38_11440
BQBJ4P68_0017310
XDIEZH22_27145
AZCAZC_3203 AZC_3892
MRDMrad2831_4776
METM446_3816
MORMOC_0480
MAQUMaq22A_c23955
MPHYMCBMB27_05504
METXA3862_29180
MINDmvi_43360
MOGMMB17_15610
RHZRHPLAN_06430 RHPLAN_06910
PLEOOHA_1_04335(trzD)
HDIHDIA_3388(trzD)
AALMLUX29_03260
BRND1F64_13985
PSINCAK95_16640 CAK95_27680
PHRC6569_17010
PSTGE8M01_10955
LIZLGH83_07300
ACUTMRB58_19190
CIDP73_3150
KREGWK63_09735
SRFLHU95_13465 LHU95_17030
SKTIGS68_15860
SMUCJL100_010175
SROEJL101_031685
FERFNB15_10190 FNB15_10205
AFRAFE_0779
AFELferr_0922
ACUAtc_m024
AFIAcife_0653
AFJAFERRID_20110
BCOBcell_1233
BLEBleG1_0675
MLITKDJ21_006895
PJDPjdr2_4927
PBDPBOR_34555
PAENP40081_35880
PAEQR50912_32525
PAEAR70723_08795
PDHB9T62_31045
PTRIKDC22_31450
PTJJRJ22_07220
SAYTPY_1352
SAPSulac_2305
MTAMoth_2120
MTHOMOTHE_c21730(trzD)
MTHZMOTHA_c22470(trzD)
NWLNWFMUON74_55550(atzD)
GORKTR9_4373
GJIH1R19_12940
DKNNHB83_00420
YIALO772_02745
MSFIT882_09430
FRIFraEuI1c_3137
PDXPsed_3188
PSERABRG53_c018
GLPGlo7428_2663 Glo7428_2665
 » show all
Reference
1  [PMID:1991731]
  Authors
Eaton RW, Karns JS
  Title
Cloning and comparison of the DNA encoding ammelide aminohydrolase and cyanuric acid amidohydrolase from three s-triazine-degrading bacterial strains.
  Journal
J Bacteriol 173:1363-6 (1991)
DOI:10.1128/JB.173.3.1363-1366.1991
  Sequence
Reference
2  [PMID:1846859]
  Authors
Eaton RW, Karns JS
  Title
Cloning and analysis of s-triazine catabolic genes from Pseudomonas sp. strain NRRLB-12227.
  Journal
J Bacteriol 173:1215-22 (1991)
DOI:10.1128/JB.173.3.1215-1222.1991
  Sequence
Reference
3  [PMID:10427042]
  Authors
Karns JS
  Title
Gene sequence and properties of an s-triazine ring-cleavage enzyme from Pseudomonas sp. strain NRRLB-12227.
  Journal
Appl Environ Microbiol 65:3512-7 (1999)
DOI:10.1128/AEM.65.8.3512-3517.1999
  Sequence
Reference
4  [PMID:12788776]
  Authors
Fruchey I, Shapir N, Sadowsky MJ, Wackett LP.
  Title
On the origins of cyanuric acid hydrolase: purification, substrates, and prevalence of AtzD from Pseudomonas sp. strain ADP.
  Journal
Appl Environ Microbiol 69:3653-7 (2003)
DOI:10.1128/AEM.69.6.3653-3657.2003
Reference
5  [PMID:29523689]
  Authors
Esquirol L, Peat TS, Wilding M, Liu JW, French NG, Hartley CJ, Onagi H, Nebl T, Easton CJ, Newman J, Scott C
  Title
An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme.
  Journal
J Biol Chem 293:7880-7891 (2018)
DOI:10.1074/jbc.RA118.001996
Other DBs
ExplorEnz - The Enzyme Database: 3.5.2.15
IUBMB Enzyme Nomenclature: 3.5.2.15
ExPASy - ENZYME nomenclature database: 3.5.2.15
UM-BBD (Biocatalysis/Biodegradation Database): 3.5.2.15
BRENDA, the Enzyme Database: 3.5.2.15
CAS: 132965-78-7

KEGG   REACTION: R12332
Entry
R12332                      Reaction                               
Name
cyanuric acid amidohydrolase
Definition
Cyanuric acid + H2O <=> 1-Carboxybiuret
Equation
Enzyme
Pathway
rn00791  Atrazine degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K03383  cyanuric acid amidohydrolase [EC:3.5.2.15]
Other DBs
RHEA: 70366

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