KEGG   ORTHOLOGY: K04126
Entry
K04126                      KO                                     
Symbol
PCBC
Name
isopenicillin-N synthase [EC:1.21.3.1]
Pathway
map00311  Penicillin and cephalosporin biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00672  Penicillin biosynthesis, aminoadipate + cycteine + valine => penicillin
M00673  Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00311 Penicillin and cephalosporin biosynthesis
    K04126  PCBC; isopenicillin-N synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K04126  PCBC; isopenicillin-N synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.21  Catalysing the reaction X-H + Y-H = X-Y
   1.21.3  With oxygen as acceptor
    1.21.3.1  isopenicillin-N synthase
     K04126  PCBC; isopenicillin-N synthase
Polyketide biosynthesis proteins [BR:ko01008]
 Nonribosomal peptide tailoring proteins
  Others
   K04126  PCBC; isopenicillin-N synthase
Other DBs
RN: R04872
GO: 0016216
Genes
BCOO: 119077147
FCD: 110843583
ANI: AN2622.2
AOR: AO090038000544
AFV: AFLA_009030
APUU: APUU_12363A
PCS: Pc21g21380(ips)
ABE: ARB_02225
TVE: TRV_00552
SCLF: BB341_07795
SCT: SCAT_5683(pcbC)
AMYY: YIM_39180(cefE1)
 » show all
Reference
PMID:7791906
  Authors
Roach PL, Clifton IJ, Fulop V, Harlos K, Barton GJ, Hajdu J, Andersson I, Schofield CJ, Baldwin JE
  Title
Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes.
  Journal
Nature 375:700-4 (1995)
DOI:10.1038/375700a0
  Sequence
[ani:AN2622.2]

KEGG   ENZYME: 1.21.3.1
Entry
EC 1.21.3.1                 Enzyme                                 
Name
isopenicillin-N synthase;
isopenicillin N synthetase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With oxygen as acceptor
Sysname
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine:oxygen oxidoreductase (cyclizing)
Reaction(IUBMB)
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O
Reaction(KEGG)
(other) R04872
Substrate
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine;
O2 [CPD:C00007]
Product
isopenicillin N [CPD:C05557];
H2O [CPD:C00001]
Comment
Forms part of the penicillin biosynthesis pathway (for pathway, click here).
History
EC 1.21.3.1 created 2002
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K04126  isopenicillin-N synthase
Genes
BCOO119077147
FCD110843583
ANIAN2622.2
AORAO090038000544
AFVAFLA_009030
APUUAPUU_12363A
PCSPc21g21380(ips)
ABEARB_02225
TVETRV_00552
SCLFBB341_07795
STIRDDW44_23090
SCTSCAT_5683(pcbC)
SCYSCATT_56830
AABA4R43_03225
AMYYYIM_39180(cefE1)
PSUUPsuf_059690
 » show all
Reference
1  [PMID:1588566]
  Authors
Huffman GW, Gesellchen PD, Turner JR, Rothenberger RB, Osborne HE, Miller FD, Chapman JL, Queener SW.
  Title
Substrate specificity of isopenicillin N synthase.
  Journal
J Med Chem 35:1897-914 (1992)
DOI:10.1021/jm00088a028
Reference
2  [PMID:7791906]
  Authors
Roach PL, Clifton IJ, Fulop V, Harlos K, Barton GJ, Hajdu J, Andersson I, Schofield CJ, Baldwin JE
  Title
Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes.
  Journal
Nature 375:700-4 (1995)
DOI:10.1038/375700a0
  Sequence
[ani:AN2622.2]
Other DBs
ExplorEnz - The Enzyme Database: 1.21.3.1
IUBMB Enzyme Nomenclature: 1.21.3.1
ExPASy - ENZYME nomenclature database: 1.21.3.1
BRENDA, the Enzyme Database: 1.21.3.1
CAS: 78642-31-6

KEGG   REACTION: R04872
Entry
R04872                      Reaction                               
Definition
delta-(L-2-Aminoadipyl)-L-cysteinyl-D-valine + Oxygen <=> Isopenicillin N + 2 H2O
Equation
Comment
multi-step reaction
Reaction class
RC01229  C05556_C05557
Enzyme
Pathway
rn00311  Penicillin and cephalosporin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00672  Penicillin biosynthesis, aminoadipate + cycteine + valine => penicillin
M00673  Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C
Orthology
K04126  isopenicillin-N synthase [EC:1.21.3.1]
Other DBs
RHEA: 22431

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