KEGG   ORTHOLOGY: K04128
Entry
K04128                      KO                                     
Symbol
cmcH
Name
hydroxymethyl cephem carbamoyltransferase [EC:2.1.3.7]
Pathway
map00311  Penicillin and cephalosporin biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00673  Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00311 Penicillin and cephalosporin biosynthesis
    K04128  cmcH; hydroxymethyl cephem carbamoyltransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K04128  cmcH; hydroxymethyl cephem carbamoyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.3  Carboxy- and carbamoyltransferases
    2.1.3.7  3-hydroxymethylcephem carbamoyltransferase
     K04128  cmcH; hydroxymethyl cephem carbamoyltransferase
Polyketide biosynthesis proteins [BR:ko01008]
 Nonribosomal peptide tailoring proteins
  Others
   K04128  cmcH; hydroxymethyl cephem carbamoyltransferase
Other DBs
RN: R05230
GO: 0047155
Genes
RSL: RPSI07_2714
RSN: RSPO_c02723(cmcH)
SCT: SCAT_5682(cmcH)
SCY: SCATT_56820
SCLF: BB341_05070 BB341_07765
THAO: NI17_008625
AAB: A4R43_03245
AMYY: YIM_39160(novN2)
Reference
PMID:7557411
  Authors
Coque JJ, Perez-Llarena FJ, Enguita FJ, Fuente JL, Martin JF, Liras P
  Title
Characterization of the cmcH genes of Nocardia lactamdurans and Streptomyces clavuligerus encoding a functional 3'-hydroxymethylcephem O-carbamoyltransferase  for cephamycin biosynthesis.
  Journal
Gene 162:21-7 (1995)
DOI:10.1016/0378-1119(95)00308-s
Reference
PMID:9696752
  Authors
Alexander DC, Jensen SE
  Title
Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein.
  Journal
J Bacteriol 180:4068-79 (1998)
DOI:10.1128/JB.180.16.4068-4079.1998
  Sequence

KEGG   ENZYME: 2.1.3.7
Entry
EC 2.1.3.7                  Enzyme                                 
Name
3-hydroxymethylcephem carbamoyltransferase
Class
Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases
Sysname
carbamoyl-phosphate:3-hydroxymethylceph-3-em-4-carboxylate carbamoyltransferase
Reaction(IUBMB)
carbamoyl phosphate + a 3-hydroxymethylceph-3-em-4-carboxylate = phosphate + a 3-carbamoyloxymethylcephem [RN:R04281]
Reaction(KEGG)
R04281 > R05230
Substrate
carbamoyl phosphate [CPD:C00169];
3-hydroxymethylceph-3-em-4-carboxylate
Product
phosphate [CPD:C00009];
3-carbamoyloxymethylcephem [CPD:C03674]
Comment
Acts on a wide range of 3-hydroxymethylcephems (a subclass of the cephalosporin antibiotics). Activated by ATP.
History
EC 2.1.3.7 created 1983
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K04128  hydroxymethyl cephem carbamoyltransferase
Genes
RSLRPSI07_2714
RSNRSPO_c02723(cmcH)
SCTSCAT_5682(cmcH)
SCYSCATT_56820
SCLFBB341_05070 BB341_07765
THAONI17_008625
AABA4R43_03245
AMYYYIM_39160(novN2)
Reference
1  [PMID:6248024]
  Authors
Brewer SJ, Taylor PM, Turner MK.
  Title
An adenosine triphosphate-dependent carbamoylphosphate--3-hydroxymethylcephem O-carbamoyltransferase from Streptomyces clavuligerus.
  Journal
Biochem J 185:555-64 (1980)
DOI:10.1042/bj1850555
Other DBs
ExplorEnz - The Enzyme Database: 2.1.3.7
IUBMB Enzyme Nomenclature: 2.1.3.7
ExPASy - ENZYME nomenclature database: 2.1.3.7
BRENDA, the Enzyme Database: 2.1.3.7
CAS: 74315-96-1

KEGG   REACTION: R05230
Entry
R05230                      Reaction                               
Definition
Deacetylcephalosporin C + Carbamoyl phosphate <=> O-Carbamoyl-deacetylcephalosporin C + Orthophosphate
Equation
Reaction class
RC00041  C03112_C06568
Enzyme
Pathway
rn00311  Penicillin and cephalosporin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00673  Cephamycin C biosynthesis, aminoadipate + cycteine + valine => cephamycin C
Orthology
K04128  hydroxymethyl cephem carbamoyltransferase [EC:2.1.3.7]

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