KEGG   ORTHOLOGY: K05394
Entry
K05394            Tight     KO                                     
Symbol
atzA
Name
atrazine chlorohydrolase [EC:3.8.1.8]
Pathway
map00791  Atrazine degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00791 Atrazine degradation
    K05394  atzA; atrazine chlorohydrolase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.8  Acting on halide bonds
   3.8.1  In carbon-halide compounds
    3.8.1.8  atrazine chlorohydrolase
     K05394  atzA; atrazine chlorohydrolase
Other DBs
RN: R05558 R05569 R06968
GO: 0018788
Genes
AG: AAC64663(atzA)
Reference
PMID:8759853
  Authors
de Souza ML, Sadowsky MJ, Wackett LP
  Title
Atrazine chlorohydrolase from Pseudomonas sp. strain ADP: gene sequence, enzyme purification, and protein characterization.
  Journal
J Bacteriol 178:4894-900 (1996)
DOI:10.1128/JB.178.16.4894-4900.1996
  Sequence
Reference
  Authors
Seffernick JL, Shapir N, Schoeb M, Johnson G, Sadowsky MJ, Wackett LP
  Title
Enzymatic degradation of chlorodiamino-s-triazine.
  Journal
Appl Environ Microbiol 68:4672-5 (2002)
DOI:10.1128/AEM.68.9.4672-4675.2002

KEGG   ENZYME: 3.8.1.8
Entry
EC 3.8.1.8                  Enzyme                                 
Name
atrazine chlorohydrolase;
AtzA
Class
Hydrolases;
Acting on halide bonds;
In carbon-halide compounds
Sysname
atrazine chlorohydrolase
Reaction(IUBMB)
atrazine + H2O = hydroxyatrazine + chloride [RN:R05558]
Reaction(KEGG)
R05558;
(other) R05569 R06968
Substrate
atrazine [CPD:C06551];
H2O [CPD:C00001]
Product
hydroxyatrazine [CPD:C06552];
chloride [CPD:C00698]
Comment
Involved in the degradation of the herbicide atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, in bacteria.
History
EC 3.8.1.8 created 2000, modified 2011
Pathway
ec00791  Atrazine degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K05394  atrazine chlorohydrolase
Reference
1  [PMID:7574646]
  Authors
de Souza ML, Wackett LP, Boundy-Mills KL, Mandelbaum RT, Sadowsky MJ
  Title
Cloning, characterization, and expression of a gene region from Pseudomonas sp. strain ADP involved in the dechlorination of atrazine.
  Journal
Appl Environ Microbiol 61:3373-8 (1995)
DOI:10.1128/AEM.61.9.3373-3378.1995
  Sequence
Reference
2  [PMID:8759853]
  Authors
de Souza ML, Sadowsky MJ, Wackett LP
  Title
Atrazine chlorohydrolase from Pseudomonas sp. strain ADP: gene sequence, enzyme purification, and protein characterization.
  Journal
J Bacteriol 178:4894-900 (1996)
DOI:10.1128/JB.178.16.4894-4900.1996
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.8.1.8
IUBMB Enzyme Nomenclature: 3.8.1.8
ExPASy - ENZYME nomenclature database: 3.8.1.8
UM-BBD (Biocatalysis/Biodegradation Database): 3.8.1.8
BRENDA, the Enzyme Database: 3.8.1.8
CAS: 168680-16-8

KEGG   REACTION: R05569
Entry
R05569                      Reaction                               
Definition
Deisopropyldeethylatrazine + H2O <=> 2-Chloro-4-hydroxy-6-amino-1,3,5-triazine + Ammonia
Equation
Comment
melaminde deaminase, TriA
Reaction class
RC01427  C06560_C08735
Enzyme
Pathway
rn00791  Atrazine degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K05394  atrazine chlorohydrolase [EC:3.8.1.8]

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