KEGG   ORTHOLOGY: K07587
Entry
K07587                      KO                                     
Symbol
sepRS
Name
O-phosphoseryl-tRNA synthetase [EC:6.1.1.27]
Pathway
map00970  Aminoacyl-tRNA biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09120 Genetic Information Processing
  09122 Translation
   00970 Aminoacyl-tRNA biosynthesis
    K07587  sepRS; O-phosphoseryl-tRNA synthetase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01007 Amino acid related enzymes
    K07587  sepRS; O-phosphoseryl-tRNA synthetase
  09182 Protein families: genetic information processing
   03016 Transfer RNA biogenesis
    K07587  sepRS; O-phosphoseryl-tRNA synthetase
Enzymes [BR:ko01000]
 6. Ligases
  6.1  Forming carbon-oxygen bonds
   6.1.1  Ligases forming aminoacyl-tRNA and related compounds
    6.1.1.27  O-phospho-L-serine---tRNA ligase
     K07587  sepRS; O-phosphoseryl-tRNA synthetase
Amino acid related enzymes [BR:ko01007]
 Aminoacyl-tRNA synthetase
  Class II (C/G)
   K07587  sepRS; O-phosphoseryl-tRNA synthetase
Transfer RNA biogenesis [BR:ko03016]
 Prokaryotic type
  Aminoacyl-tRNA synthetases (AARSs)
   Other AARSs
    K07587  sepRS; O-phosphoseryl-tRNA synthetase
Aminoacyl-tRNA synthetases [br01613.html]
 Aminoacyl-tRNA synthetases in prokaryotes and eukaryotes
  K07587
Other DBs
RN: R08576
COG: COG2024
GO: 0043816
Genes
MJA: MJ_1660
MFE: Mefer_0735
MVU: Metvu_1155
MFS: MFS40622_0592
MIF: Metin_0235
MJH: JH146_1468
MESG: MLAUSG7_1103(sepS)
MESA: MLASG1_0675(sepS)
MIG: Metig_1346
MMP: MMP0688
MMD: GYY_02815
MMAK: MMKA1_07400(sepS)
MMAO: MMOS7_07340(sepS)
MMAD: MMJJ_02890(sepS)
MAE: Maeo_1060
MVO: Mvol_0433
METF: CFE53_05100(sepS)
MTH: MTH_1501
MMG: MTBMA_c00860(pheS1)
MWO: MWSIV6_0042(sepS)
MTEE: MTTB_00760
METC: MTCT_1365
METE: tca_01448(pheS_2)
METK: FVF72_04105(sepS)
MTHM: FZP57_00155(sepS)
METH: MBMB1_0036(sepS)
MFC: BRM9_0091(sepS)
MFI: DSM1535_1409(sepS)
MCUB: MCBB_0052(sepS)
METT: CIT01_05855(sepS)
METO: CIT02_06885(sepS)
MEME: HYG87_06790(sepS)
MFV: Mfer_0342
MKA: MK0439
AFU: AF_0110
FPL: Ferp_0952
GAC: GACE_0149
GAH: GAH_00998
MBAR: MSBR2_3396
MBAK: MSBR3_3408
MAC: MA_0090(pheRS)
MMA: MM_1383
MMAC: MSMAC_3298
METM: MSMTP_3173
MTHR: MSTHT_1883
MTHE: MSTHC_1401
MHOR: MSHOH_4028
MBU: Mbur_0923
MMET: MCMEM_1431
MELO: J7W08_05865(sepS)
MMH: Mmah_0685
MPY: Mpsy_1453
MTP: Mthe_0363
MCJ: MCON_3135(sepS)
MHI: Mhar_0928
MHU: Mhun_2839
MRTJ: KHC33_02760(sepS)
MLA: Mlab_0537
MEMA: MMAB1_1853(sepS)
MSUM: OH143_05355(sepS)
MPI: Mpet_0614
MEND: L6E24_05485(sepS)
MAQE: RJ40_06815
MOU: OU421_07565(sepS)
MBN: Mboo_0273
MPL: Mpal_0290
MPD: MCP_1457
MEZ: Mtc_1161
RCI: RRC273
MELU: MTLP_06560
BARB: AOA66_0046(sepS)
 » show all
Reference
  Authors
Sheppard K, Yuan J, Hohn MJ, Jester B, Devine KM, Soll D
  Title
From one amino acid to another: tRNA-dependent amino acid biosynthesis.
  Journal
Nucleic Acids Res 36:1813-25 (2008)
DOI:10.1093/nar/gkn015
Reference
  Authors
Fukunaga R, Yokoyama S
  Title
Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea.
  Journal
Nat Struct Mol Biol 14:272-9 (2007)
DOI:10.1038/nsmb1219
  Sequence
[mja:MJ_1660] [afu:AF_0110]

KEGG   ENZYME: 6.1.1.27
Entry
EC 6.1.1.27                 Enzyme                                 
Name
O-phospho-L-serine---tRNA ligase;
O-phosphoseryl-tRNA ligase;
non-canonical O-phosphoseryl-tRNA synthetase;
SepRS
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
Sysname
O-phospho-L-serine:tRNACys ligase (AMP-forming)
Reaction(IUBMB)
ATP + O-phospho-L-serine + tRNACys = AMP + diphosphate + O-phospho-L-seryl-tRNACys [RN:R08576]
Reaction(KEGG)
R08576
Substrate
ATP [CPD:C00002];
O-phospho-L-serine [CPD:C01005];
tRNA(Cys) [CPD:C01639]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
O-phospho-L-seryl-tRNACys
Comment
In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase) converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [1]. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase) [2].
History
EC 6.1.1.27 created 2009
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K07587  O-phosphoseryl-tRNA synthetase
Genes
MJAMJ_1660
MFEMefer_0735
MVUMetvu_1155
MFSMFS40622_0592
MIFMetin_0235
MJHJH146_1468
MESGMLAUSG7_1103(sepS)
MESAMLASG1_0675(sepS)
MIGMetig_1346
MMPMMP0688
MMQMmarC5_0888
MMXMmarC6_0196
MMZMmarC7_1715
MMDGYY_02815
MMAKMMKA1_07400(sepS)
MMAOMMOS7_07340(sepS)
MMADMMJJ_02890(sepS)
MAEMaeo_1060
MVNMevan_1570
MVOMvol_0433
MOKMetok_0981
METFCFE53_05100(sepS)
MTHMTH_1501
MMGMTBMA_c00860(pheS1)
MWOMWSIV6_0042(sepS)
MTEEMTTB_00760
METCMTCT_1365
METEtca_01448(pheS_2)
METZMETMT2_1346
METKFVF72_04105(sepS)
MTHMFZP57_00155(sepS)
MAROMarbSA_11170
MELMetbo_0044
MEWMSWAN_0057
METHMBMB1_0036(sepS)
MFCBRM9_0091(sepS)
MFIDSM1535_1409(sepS)
MCUBMCBB_0052(sepS)
MSUBBK009_09900
METNBK008_05560
METTCIT01_05855(sepS)
METOCIT02_06885(sepS)
MEMEHYG87_06790(sepS)
MFVMfer_0342
MKAMK0439
AFUAF_0110
AFGAFULGI_00001070
APOArcpr_1311
AVEArcve_1107
ASTAsulf_01133
FPLFerp_0952
GACGACE_0149
GAHGAH_00998
MBAMbar_A1204
MBYMSBRM_3441
MBWMSBRW_3520
MBARMSBR2_3396
MBAKMSBR3_3408
MACMA_0090(pheRS)
MMAMM_1383
MMAZMmTuc01_1435
MMJMSMAS_3279
MMACMSMAC_3298
MVCMSVAZ_3488
MEKMSKOL_3456
MLSMSLAZ_3154
METMMSMTP_3173
MEFMSWH1_3131
MEQMSWHS_3343
MSJMSSAC_0069
MSZMSSIH_0062
MSWMSSIT_0062
MTHRMSTHT_1883
MTHEMSTHC_1401
MHORMSHOH_4028
MFZAOB57_008705
MBUMbur_0923
MMETMCMEM_1431
MELOJ7W08_05865(sepS)
MMHMmah_0685
MHAZBHR79_10180
MEVMetev_1721
MZHMzhil_0247
MPYMpsy_1453
MZIHWN40_01550
MHZMetho_0248
MTPMthe_0363
MCJMCON_3135(sepS)
MHIMhar_0928
MHUMhun_2839
MRTJKHC33_02760(sepS)
MLAMlab_0537
MEMMemar_2215
MBGBN140_1447
MEMAMMAB1_1853(sepS)
MCHKMchiMG62_25320
MSUMOH143_05355(sepS)
MPIMpet_0614
MENDL6E24_05485(sepS)
MAQERJ40_06815
MOUOU421_07565(sepS)
MBNMboo_0273
MFOMetfor_2843
MPLMpal_0290
MPDMCP_1457
MEZMtc_1161
RCIRRC273
MELUMTLP_06560
BARBAOA66_0046(sepS)
 » show all
Reference
1  [PMID:17351629]
  Authors
Fukunaga R, Yokoyama S
  Title
Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea.
  Journal
Nat Struct Mol Biol 14:272-9 (2007)
DOI:10.1038/nsmb1219
  Sequence
[mja:MJ_1660] [afu:AF_0110]
Reference
2  [PMID:18559341]
  Authors
Hauenstein SI, Perona JJ
  Title
Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei.
  Journal
J Biol Chem 283:22007-17 (2008)
DOI:10.1074/jbc.M801839200
Other DBs
ExplorEnz - The Enzyme Database: 6.1.1.27
IUBMB Enzyme Nomenclature: 6.1.1.27
ExPASy - ENZYME nomenclature database: 6.1.1.27
BRENDA, the Enzyme Database: 6.1.1.27

KEGG   REACTION: R08576
Entry
R08576                      Reaction                               
Name
O-phospho-L-serine:tRNA(Cys) ligase (AMP-forming)
Definition
O-Phospho-L-serine + tRNA(Cys) + ATP <=> O-Phosphoseryl-tRNA(Cys) + Diphosphate + AMP
Equation
Comment
O-phosphoseryl-tRNA synthetase
Reaction class
RC00055  C01639_C17022
RC00523  C01005_C17022
Enzyme
Pathway
rn00970  Aminoacyl-tRNA biosynthesis
Orthology
K07587  O-phosphoseryl-tRNA synthetase [EC:6.1.1.27]
Other DBs
RHEA: 25681

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