KEGG   ORTHOLOGY: K10224
Entry
K10224                      KO                                     
Symbol
FADS1
Name
acyl-CoA (8-3)-desaturase (Delta-5 desaturase) [EC:1.14.19.44]
Pathway
map01040  Biosynthesis of unsaturated fatty acids
map01100  Metabolic pathways
map01212  Fatty acid metabolism
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   01040 Biosynthesis of unsaturated fatty acids
    K10224  FADS1; acyl-CoA (8-3)-desaturase (Delta-5 desaturase)
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01004 Lipid biosynthesis proteins
    K10224  FADS1; acyl-CoA (8-3)-desaturase (Delta-5 desaturase)
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.44  acyl-CoA (8-3)-desaturase
     K10224  FADS1; acyl-CoA (8-3)-desaturase (Delta-5 desaturase)
Lipid biosynthesis proteins [BR:ko01004]
 Desaturase
  Front-end
   K10224  FADS1; acyl-CoA (8-3)-desaturase (Delta-5 desaturase)
Other DBs
RN: R11059 R11060 R12169
Genes
HSA: 3992(FADS1)
PTR: 451243(FADS1)
PPS: 100981162(FADS1)
GGO: 101136185(FADS1)
PON: 100436159(FADS1)
NLE: 100596120(FADS1)
MCC: 722343(FADS1)
MCF: 101864778(FADS1)
CSAB: 103234224(FADS1)
CATY: 105577579(FADS1)
PANU: 100126707(FADS1)
TGE: 112606126(FADS1)
RRO: 104679375(FADS1)
RBB: 108533599(FADS1)
TFN: 117080259(FADS1)
PTEH: 111524317(FADS1)
CJC: 100389579(FADS1)
SBQ: 101052172(FADS1)
MMUR: 105878618(FADS1)
LCAT: 123641269(FADS1)
OGA: 100954652(FADS1)
MMU: 76267(Fads1)
MCAL: 110285897(Fads1)
MPAH: 110322451(Fads1)
RNO: 84575(Fads1)
MCOC: 116103544(Fads1)
MUN: 110548091(Fads1)
CGE: 100773095
MAUA: 101844332(Fads1)
PLEU: 114703435(Fads1)
MORG: 121453877(Fads1)
AAMP: 119813303(Fads1)
NGI: 103729124(Fads1)
HGL: 101698014(Fads1)
CPOC: 100723348(Fads1)
CCAN: 109685566(Fads1)
DORD: 105995514(Fads1)
DSP: 122104887(Fads1)
NCAR: 124958643
OCU: 100342403(FADS1)
OPI: 101524219(FADS1)
TUP: 102500595(FADS1)
CFA: 483793(FADS1)
CLUD: 112659419(FADS1)
VVP: 112911088(FADS1)
VLG: 121472259(FADS1)
AML: 100472955(FADS1)
UMR: 103678230(FADS1)
UAH: 113246984(FADS1)
UAR: 123785000(FADS1)
ELK: 111149587
LLV: 125078519
MPUF: 101676081(FADS1)
ORO: 101364825(FADS1)
EJU: 114220205(FADS1)
ZCA: 113914860(FADS1)
MLX: 118015924(FADS1)
FCA: 101083966(FADS1)
PYU: 121043898(FADS1)
PBG: 122483865(FADS1)
PTG: 102968608(FADS1)
PPAD: 109246555(FADS1)
AJU: 106986968(FADS1)
HHV: 120246150(FADS1)
BTA: 533107(FADS1)
BOM: 102276098(FADS1)
BIU: 109554369(FADS1)
BBUB: 102405648(FADS1)
CHX: 102178247(FADS1)
OAS: 101109049(FADS1)
ODA: 120874548(FADS1)
CCAD: 122431165(FADS1)
SSC: 444995(FADS1)
CFR: 102506763(FADS1)
CBAI: 105070110(FADS1)
CDK: 105094872(FADS1)
VPC: 102543424(FADS1)
BACU: 103008410(FADS1)
LVE: 103082696(FADS1)
OOR: 101275971(FADS1)
DLE: 111183884(FADS1)
PCAD: 102988776(FADS1)
PSIU: 116757880(FADS1)
ECB: 102150261(FADS1)
EPZ: 103543089(FADS1)
EAI: 106847567(FADS1)
MYB: 102261592(FADS1)
MYD: 102773181(FADS1)
MMYO: 118649002(FADS1)
MNA: 107527686(FADS1)
PKL: 118707199(FADS1)
HAI: 109390020(FADS1)
DRO: 112317234(FADS1)
SHON: 118996919(FADS1)
AJM: 119043682(FADS1)
PDIC: 114499452(FADS1)
PHAS: 123829519(FADS1)
MMF: 118627540(FADS1)
RFQ: 117030354(FADS1)
PALE: 102894313(FADS1)
PGIG: 120600651(FADS1)
PVP: 105297785(FADS1)
RAY: 107501590(FADS1)
MJV: 108392394(FADS1)
TOD: 119231397 119250072(FADS1)
SARA: 101540344(FADS1)
LAV: 100677495
TMU: 101347278
DNM: 101417194(FADS1)
MDO: 100028492(FADS1)
SHR: 100922163(FADS1)
PCW: 110195308(FADS1)
OAA: 103171425(FADS1)
GGA: 423119(FADS1L2) 423120(FADS1) 428851(FADS1L1)
FCH: 102049758(FADS1) 106631119
OHA: 104339400 104339408(FADS1)
GGN: 109298190
CPIC: 101936861(FADS1) 101937159
XLA: 108713836(fads1.L) 108715199(fads1.S)
XTR: 100489232(fads1)
NPR: 108794178(FADS1)
RTEM: 120917259(FADS1)
BBUF: 120981188(FADS1)
BGAR: 122920198(FADS1)
AANG: 118215203
LOC: 102691516(fads1)
PSPA: 121330344
LCM: 102350226(FADS1)
CMK: 103174860
RTP: 109937249
ATD: 109598043
TNL: 113500734
PCAN: 112557826
 » show all
Reference
  Authors
Cho HP, Nakamura M, Clarke SD
  Title
Cloning, expression, and fatty acid regulation of the human delta-5 desaturase.
  Journal
J Biol Chem 274:37335-9 (1999)
DOI:10.1074/jbc.274.52.37335
  Sequence
[hsa:3992]

KEGG   ENZYME: 1.14.19.44
Entry
EC 1.14.19.44               Enzyme                                 
Name
acyl-CoA (8-3)-desaturase;
FADS1 (gene name);
acyl-CoA 5-desaturase (methylene-interrupted)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
Delta8-acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (5,6-cis-dehydrogenating)
Reaction(IUBMB)
(1) (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = arachidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11059];
(2) (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11060]
Reaction(KEGG)
R11059 R11060;
(other) R12169
Substrate
(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-CoA [CPD:C03595];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
(8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-CoA [CPD:C16164]
Product
arachidonoyl-CoA [CPD:C02249];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-CoA [CPD:C16165]
Comment
The enzyme introduces a cis double bond at carbon 5 of acyl-CoAs that contain a double bond at position 8. The enzymes from algae, mosses, mammals and the protozoan Leishmania major catalyse the desaturation of dihomo-gamma-linoleate [(8Z,11Z,14Z)-icosa-8,11,14-trienoate] and (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoate to generate arachidonate and (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoate, respectively. The enzyme contains a cytochrome b5 domain that acts as the direct electron donor to the desaturase active site and does not require an external cytochrome. cf. EC 1.14.19.37, acyl-CoA 5-desaturase.
History
EC 1.14.19.44 created 2015
Pathway
ec01040  Biosynthesis of unsaturated fatty acids
Orthology
K10224  acyl-CoA (8-3)-desaturase (Delta-5 desaturase)
Genes
HSA3992(FADS1)
PTR451243(FADS1)
PPS100981162(FADS1)
GGO101136185(FADS1)
PON100436159(FADS1)
NLE100596120(FADS1)
MCC722343(FADS1)
MCF101864778(FADS1)
CSAB103234224(FADS1)
CATY105577579(FADS1)
PANU100126707(FADS1)
TGE112606126(FADS1)
RRO104679375(FADS1)
RBB108533599(FADS1)
TFN117080259(FADS1)
PTEH111524317(FADS1)
CJC100389579(FADS1)
SBQ101052172(FADS1)
MMUR105878618(FADS1)
LCAT123641269(FADS1)
OGA100954652(FADS1)
MMU76267(Fads1)
MCAL110285897(Fads1)
MPAH110322451(Fads1)
RNO84575(Fads1)
MCOC116103544(Fads1)
MUN110548091(Fads1)
CGE100773095
MAUA101844332(Fads1)
PLEU114703435(Fads1)
MORG121453877(Fads1)
AAMP119813303(Fads1)
NGI103729124(Fads1)
HGL101698014(Fads1)
CPOC100723348(Fads1)
CCAN109685566(Fads1)
DORD105995514(Fads1)
DSP122104887(Fads1)
NCAR124958643
OCU100342403(FADS1)
OPI101524219(FADS1)
TUP102500595(FADS1)
CFA483793(FADS1)
CLUD112659419(FADS1)
VVP112911088(FADS1)
VLG121472259(FADS1)
AML100472955(FADS1)
UMR103678230(FADS1)
UAH113246984(FADS1)
UAR123785000(FADS1)
ELK111149587
LLV125078519
MPUF101676081(FADS1)
ORO101364825(FADS1)
EJU114220205(FADS1)
ZCA113914860(FADS1)
MLX118015924(FADS1)
FCA101083966(FADS1)
PYU121043898(FADS1)
PBG122483865(FADS1)
PTG102968608(FADS1)
PPAD109246555(FADS1)
AJU106986968(FADS1)
HHV120246150(FADS1)
BTA533107(FADS1)
BOM102276098(FADS1)
BIU109554369(FADS1)
BBUB102405648(FADS1)
CHX102178247(FADS1)
OAS101109049(FADS1)
ODA120874548(FADS1)
CCAD122431165(FADS1)
SSC444995(FADS1)
CFR102506763(FADS1)
CBAI105070110(FADS1)
CDK105094872(FADS1)
VPC102543424(FADS1)
BACU103008410(FADS1)
LVE103082696(FADS1)
OOR101275971(FADS1)
DLE111183884(FADS1)
PCAD102988776(FADS1)
PSIU116757880(FADS1)
ECB102150261(FADS1)
EPZ103543089(FADS1)
EAI106847567(FADS1)
MYB102261592(FADS1)
MYD102773181(FADS1)
MMYO118649002(FADS1)
MNA107527686(FADS1)
PKL118707199(FADS1)
HAI109390020(FADS1)
DRO112317234(FADS1)
SHON118996919(FADS1)
AJM119043682(FADS1)
PDIC114499452(FADS1)
PHAS123829519(FADS1)
MMF118627540(FADS1)
RFQ117030354(FADS1)
PALE102894313(FADS1)
PGIG120600651(FADS1)
PVP105297785(FADS1)
RAY107501590(FADS1)
MJV108392394(FADS1)
TOD119231397 119250072(FADS1)
SARA101540344(FADS1)
LAV100677495
TMU101347278
DNM101417194(FADS1)
MDO100028492(FADS1)
SHR100922163(FADS1)
PCW110195308(FADS1)
OAA103171425(FADS1)
GGA423119(FADS1L2) 423120(FADS1) 428851(FADS1L1)
PCOC116239003 116239007 116239016
MGP100549516 100549671 100549825(FADS1)
CJO107314410 107314438 107314472
NMEL110400732 110400736 110401255(FADS1)
APLA101789732 101799007(FADS1) 101799800
ACYG106033871(FADS1) 106044856 106044871
AFUL116489595 116489696(FADS1) 116490009
TGU100225746(FADS1) 100228631 115495653
LSR110484927(FADS1) 110485021 110485022
SCAN103822124(FADS1) 103822125 103822228
PMOA120499563 120499565(FADS1) 120499600
OTC121339155(FADS1) 121339445 121339494
PRUF121354751(FADS1) 121354752 121354755
GFR102031677 102040395(FADS1) 102040846
FAB101809325 101809791(FADS1) 101813351
PHI102101859 102112391(FADS1) 102112775
PMAJ107205341 107205403(FADS1) 107205404
CCAE111930634 111930636 111930637
CCW104697619 104697620 104697621
CBRC103614699 103614730(FADS1) 103614731
ETL114066084 114066085 114066089
ZAB102066550 102069423 102069595(FADS1)
FPG101910435 101912001(FADS1) 106112851
FCH102049758(FADS1) 106631119
CLV102094615 102098315(FADS1) 102098790
EGZ104124680 104124682 104124699
NNI104009395 104009483(FADS1) 104009484
PLET104619104 104627919 104627920
PCRI104023942 104036418 104037506
ACUN113481253 113481341 113481355(FADS1)
TALA104363221 104368248 104368249(FADS1)
PADL103925784 103925826 103925828(FADS1)
ACHC115351609 115352165 115352167
HALD104313152 104313153 104322733
CCRI104162789 104162790 104166166
CSTI104554377 104554378 104562342
EHS104502732 104504142 104505341
CMAC104473140 104477798 104477799
FGA104069286 104070764
GSTE104253491 104253492 104261229
LDI104349394 104351594 104351595
MNB103769636 103781635 103781636
OHA104339400 104339408(FADS1)
AAM106490039 106490040 106490045(FADS1)
AROW112978023(FADS1) 112978027 112978037
NPD112948005(FADS1) 112948043 112948044
DNE112991679 112991686 112991696(FADS1)
ASN102378297 102382011 102382251 102382503 102382756 102386118 102387601 102388109
AMJ102563927 102564163 102564392 102564994 102565222(FADS1) 102574216 106737005
CPOO109318130 109318222
GGN109298190
PSS102460779 102461025
CMY102932384 102932824 102933055
CPIC101936861(FADS1) 101937159
TST117876524 117877424
CABI116827619 116828643 116830901(FADS1)
MRV120403578 120403579 120403581 120403991
ACS100551618(fads1) 100555351 100555547 100555740 100555937
PVT110086171 110086173 110086190 110086192(FADS1) 110086194 110086197
SUND121926305 121930479 121930494 121930500 121930504
PBI103050792(FADS1) 103053493 103065518
PMUR107287991 107291623 107291625 107291626 107291627 107291628 107291629 107291630 107295117
TSR106538435(FADS1) 106539606 106554505
PGUT117667020 117667022 117667025 117667027 117667030
VKO123021210 123021212 123021213 123021347 123021348
PMUA114584095 114584100 114600543 114600551 114600556 114600565
ZVI118089033 118089041 118089045 118089057 118089062
GJA107108850 107108852(FADS1) 107121912 107121913 107121914
STOW125426611 125426612 125427059 125427338 125427467 125427539
XLA108713836(fads1.L) 108715199(fads1.S)
XTR100489232(fads1)
NPR108794178(FADS1)
RTEM120917259(FADS1)
BBUF120981188(FADS1)
BGAR122920198(FADS1)
AANG118215203
LOC102691516(fads1)
PSPA121330344
LCM102350226(FADS1)
CMK103174860
RTP109937249
ATD109598043
TNL113500734
LGILOTGIDRAFT_170234
PCAN112557826
GTTGUITHDRAFT_104142
 » show all
Reference
1  [PMID:10601301]
  Authors
Cho HP, Nakamura M, Clarke SD
  Title
Cloning, expression, and fatty acid regulation of the human delta-5 desaturase.
  Journal
J Biol Chem 274:37335-9 (1999)
DOI:10.1074/jbc.274.52.37335
  Sequence
[hsa:3992]
Reference
2  [PMID:10769175]
  Authors
Leonard AE, Kelder B, Bobik EG, Chuang LT, Parker-Barnes JM, Thurmond JM, Kroeger PE, Kopchick JJ, Huang YS, Mukerji P
  Title
cDNA cloning and characterization of human Delta5-desaturase involved in the biosynthesis of arachidonic acid.
  Journal
Biochem J 347 Pt 3:719-24 (2000)
  Sequence
[hsa:3992]
Reference
3  [PMID:16403015]
  Authors
Tripodi KE, Buttigliero LV, Altabe SG, Uttaro AD
  Title
Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan.
  Journal
FEBS J 273:271-80 (2006)
DOI:10.1111/j.1742-4658.2005.05049.x
Reference
4  [PMID:21193673]
  Authors
Tavares S, Grotkjaer T, Obsen T, Haslam RP, Napier JA, Gunnarsson N
  Title
Metabolic engineering of Saccharomyces cerevisiae for production of Eicosapentaenoic Acid, using a novel {Delta}5-Desaturase from Paramecium tetraurelia.
  Journal
Appl Environ Microbiol 77:1854-61 (2011)
DOI:10.1128/AEM.01935-10
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.44
IUBMB Enzyme Nomenclature: 1.14.19.44
ExPASy - ENZYME nomenclature database: 1.14.19.44
BRENDA, the Enzyme Database: 1.14.19.44

KEGG   REACTION: R11060
Entry
R11060                      Reaction                               
Name
(8Z,11Z,14Z,17Z)-icosatetraenoyl-CoA,ferrocytochrome-b5:oxygen oxidoreductase (5,6-cis-dehydrogenating)
Definition
(8Z,11Z,14Z,17Z)-Icosatetraenoyl-CoA + 2 Ferrocytochrome b5 + Oxygen + 2 H+ <=> (5Z,8Z,11Z,14Z,17Z)-Icosapentaenoyl-CoA + 2 Ferricytochrome b5 + 2 H2O
Equation
C16164 + 2 C00999 + C00007 + 2 C00080 <=> C16165 + 2 C00996 + 2 C00001
Reaction class
RC00917  C16164_C16165
Enzyme
Pathway
rn01040  Biosynthesis of unsaturated fatty acids
rn01212  Fatty acid metabolism
Orthology
K10224  acyl-CoA (8-3)-desaturase (Delta-5 desaturase) [EC:1.14.19.44]
Other DBs
RHEA: 46423

DBGET integrated database retrieval system