KEGG   ORTHOLOGY: K11336
Entry
K11336                      KO                                     
Symbol
bchF
Name
3-vinyl bacteriochlorophyllide hydratase [EC:4.2.1.165]
Pathway
map00860  Porphyrin metabolism
map01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00860 Porphyrin metabolism
    K11336  bchF; 3-vinyl bacteriochlorophyllide hydratase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.165  chlorophyllide a 31-hydratase
     K11336  bchF; 3-vinyl bacteriochlorophyllide hydratase
Other DBs
RN: R09054 R09059
Genes
ALV: Alvin_2643
ATEP: Atep_05730(bchF)
TVI: Thivi_2411
TMB: Thimo_3026
MPUR: MARPU_13965
TSY: THSYN_31440
TTP: E6P07_13140(bchF)
THIP: N838_12635(bchF)
CJAP: GWK36_13325(bchF)
THIM: KFB96_13280(bchF)
TBOG: LT988_09000(bchF)
HHA: Hhal_1638
PANI: DCO16_03385(bchF)
PCOS: C2747_06875(bchF)
HYR: BSY239_1283(bchF)
HCZ: G9Q37_21695(bchF)
METP: C1M51_03465(bchF)
RGE: RGE_33450(bchF)
ATER: MW290_02685(bchF)
METR: BSY238_2468(bchF)
RPOD: E0E05_11905(bchF)
AGC: BSY240_3645(bchF)
RROS: D4A92_02750(bchF)
ABAW: D5400_05050(bchF)
PDES: FE840_002440(bchF)
BRA: BRADO1638(bchF)
BBT: BBta_6418(bchF)
BRS: S23_19370(bchF)
AOL: S58_59000
BRO: BRAD285_5763(bchF)
BRQ: CIT40_23845(bchF)
BGZ: XH91_02735(bchF)
BBET: F8237_24770(bchF)
RPA: RPA1541(bchF)
RPB: RPB_3982
RPC: RPC_1312
RPD: RPD_3737
RPE: RPE_1346
RPT: Rpal_1729
BOS: BSY19_4034(bchF)
MEA: Mex_1p5274(bchF)
MDI: METDI5874(bchF)
MEX: Mext_4817
MCH: Mchl_5284
MPO: Mpop_5362
MET: M446_3717
MOR: MOC_2253(bchF)
META: Y590_24615
MEE: DA075_04045(bchF)
METD: C0214_26355(bchF)
METS: DK389_01075(bchF)
METI: DK427_18030(bchF)
MMES: MMSR116_22245(bchF)
MTEA: DK419_05005(bchF)
MIND: mvi_09160
MOG: MMB17_13850(bchF)
MTAD: M6G65_04535(bchF)
MSL: Msil_2043
BBAR: RHAL1_00839(bchF)
RVA: Rvan_0999
BVR: BVIR_569
BLAG: BLTE_02050(bchF)
MROS: EHO51_17165(bchF)
AUB: LXB15_06275(bchF)
JAN: Jann_0162(bchF)
RDE: RD1_0140(bchF)
RLI: RLO149_p940700(bchF)
RPON: G3256_18980(bchF)
DSH: Dshi_3533(bchF)
ROM: EI983_05555(bchF)
THAA: CFI11_23835(bchF)
RMH: LVO79_15075(bchF)
MALU: KU6B_16680(bchF)
RSP: RSP_0284(bchF)
CAZT: LV780_04400(bchF)
RCP: RCAP_rcc00666(bchF)
PTP: RCA23_c29930(bchF)
RSU: NHU_00210(bchF)
RHC: RGUI_1987
RBG: BG454_02925(bchF)
GEH: HYN69_05420(bchF)
TAW: EI545_18840(bchF)
POZ: I0K15_16890(bchF)
NSM: JO391_16360(bchF)
FAQ: G5B39_09990(bchF)
NOT: C7W88_00555(bchF)
NGF: FRF71_09220(bchF)
NOG: GKE62_08510(bchF)
SPHI: TS85_23010
SSAN: NX02_18330
SCHY: GVO57_11520(bchF)
SAER: NEF64_07790(bchF)
BLAS: BSY18_2709(bchF)
PALG: HFP57_06875(bchF)
PARJ: J4G78_07460(bchF)
SAND: H3309_05815(bchF)
POQ: KZX46_18600(bchF)
PMEA: KTC28_16910(bchF)
ALH: G6N82_11510(bchF)
ERK: CD351_13945(bchF)
PHZ: CHX26_14570(bchF)
POT: E2E27_07265(bchF)
AMV: ACMV_18950(bchF)
SRF: LHU95_13985(bchF)
RAP: RHOA_2397
NCB: C0V82_18050(bchF)
SKT: IGS68_17000(bchF)
SMUC: JL100_009045(bchF)
SROE: JL101_015170(bchF)
RRU: Rru_A0624
RRF: F11_03200
RCE: RC1_2116(bchF)
RPM: RSPPHO_00022(bchF)
ELIO: KO353_02790(bchF)
RCA: Rcas_3748
CAU: Caur_0415
CAG: Cagg_3129
CVL: J8C06_12190(bchF)
CHLO: J8C02_13075(bchF)
GGR: HKW67_08900(bchF)
CTE: CT1421(bchF)
CPC: Cpar_0743
CCH: Cag_0402
CLI: Clim_0899
PVI: Cvib_1239
PLT: Plut_1421
PPH: Ppha_0981
PAA: Paes_1533
PROS: CHL67_03610(bchF)
 » show all
Reference
  Authors
Kim EJ, Kim JS, Lee IH, Rhee HJ, Lee JK
  Title
Superoxide generation by chlorophyllide a reductase of Rhodobacter sphaeroides.
  Journal
J Biol Chem 283:3718-30 (2008)
DOI:10.1074/jbc.M707774200
Reference
PMID:8385667
  Authors
Burke DH, Alberti M, Hearst JE
  Title
bchFNBH bacteriochlorophyll synthesis genes of Rhodobacter capsulatus and identification of the third subunit of light-independent protochlorophyllide reductase in bacteria and plants.
  Journal
J Bacteriol 175:2414-22 (1993)
DOI:10.1128/JB.175.8.2414-2422.1993
  Sequence

KEGG   ENZYME: 4.2.1.165
Entry
EC 4.2.1.165                Enzyme                                 
Name
chlorophyllide a 31-hydratase;
bchF (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
chlorophyllide-a 31-hydro-lyase
Reaction(IUBMB)
(1) 3-devinyl-3-(1-hydroxyethyl)chlorophyllide a = chlorophyllide a + H2O [RN:R09059];
(2) 3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a = 3-deacetyl-3-vinylbacteriochlorophyllide a + H2O [RN:R09054]
Reaction(KEGG)
R09054 R09059
Substrate
3-devinyl-3-(1-hydroxyethyl)chlorophyllide a [CPD:C18154];
3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a [CPD:C18153]
Product
chlorophyllide a [CPD:C02139];
H2O [CPD:C00001];
3-deacetyl-3-vinylbacteriochlorophyllide a [CPD:C18152]
Comment
The enzyme, together with EC 1.3.7.15, chlorophyllide-a reductase, and EC 1.1.1.396, bacteriochlorophyllide-a dehydrogenase, is involved in the conversion of chlorophyllide a to bacteriochlorophyllide a. The enzymes can act in multiple orders, resulting in the formation of different intermediates, but the final product of the cumulative action of the three enzymes is always bacteriochlorophyllide a. The enzyme catalyses the hydration of a vinyl group on ring A, converting it to a hydroxyethyl group.
History
EC 4.2.1.165 created 2016
Pathway
ec00860  Porphyrin metabolism
ec01110  Biosynthesis of secondary metabolites
Orthology
K11336  3-vinyl bacteriochlorophyllide hydratase
Genes
ALVAlvin_2643
ATEPAtep_05730(bchF)
TVIThivi_2411
TMBThimo_3026
MPURMARPU_13965
TSYTHSYN_31440
TTPE6P07_13140(bchF)
THIPN838_12635(bchF)
CJAPGWK36_13325(bchF)
THIMKFB96_13280(bchF)
TBOGLT988_09000(bchF)
HHAHhal_1638
HHKHH1059_06050
HHCM911_08235
EBSECTOBSL9_2976
PDQCL55_00013430
PANIDCO16_03385(bchF)
PCOSC2747_06875(bchF)
RACRA876_06135
LIML103DPR2_01003
LIHL63ED372_00659
HYRBSY239_1283(bchF)
HCZG9Q37_21695(bchF)
METPC1M51_03465(bchF)
RGERGE_33450(bchF)
RBNRBXJA2T_09567
RDPRD2015_1015
ATERMW290_02685(bchF)
METRBSY238_2468(bchF)
BEBAEM42_07585
RPODE0E05_11905(bchF)
AGCBSY240_3645(bchF)
RROSD4A92_02750(bchF)
ABAWD5400_05050(bchF)
PDESFE840_002440(bchF)
BRABRADO1638(bchF)
BBTBBta_6418(bchF)
BRSS23_19370(bchF)
AOLS58_59000
BROBRAD285_5763(bchF)
BRQCIT40_23845(bchF)
BGZXH91_02735(bchF)
BBETF8237_24770(bchF)
RPARPA1541(bchF)
RPBRPB_3982
RPCRPC_1312
RPDRPD_3737
RPERPE_1346
RPTRpal_1729
RPXRpdx1_3979
BOSBSY19_4034(bchF)
MEAMex_1p5274(bchF)
MDIMETDI5874(bchF)
MEXMext_4817
MCHMchl_5284
MPOMpop_5362
MZAB2G69_06070
MRDMrad2831_1849
METM446_3717
MORMOC_2253(bchF)
METAY590_24615
MAQUMaq22A_c01675
MPHYMCBMB27_01471
MEEDA075_04045(bchF)
METDC0214_26355(bchF)
METXA3862_21680
METSDK389_01075(bchF)
METIDK427_18030(bchF)
MMESMMSR116_22245(bchF)
MTEADK419_05005(bchF)
MINDmvi_09160
MOGMMB17_13850(bchF)
MTADM6G65_04535(bchF)
MSLMsil_2043
BBARRHAL1_00839(bchF)
RVARvan_0999
BVRBVIR_569
BLAGBLTE_02050(bchF)
MROSEHO51_17165(bchF)
AUZSa4125_27530
AUBLXB15_06275(bchF)
BSBBresu_3063
BRLBZG35_15535
RUTFIU92_10035
JANJann_0162(bchF)
RDERD1_0140(bchF)
RLIRLO149_p940700(bchF)
RPONG3256_18980(bchF)
DSHDshi_3533(bchF)
REDroselon_01095
SUAMBOO69_19195
TOMBWR18_20070
RMMROSMUCSMR3_00880
ROKRAK1035_3750
RIDRIdsm_01780
ROMEI983_05555(bchF)
ROHFIU89_14095
THAACFI11_23835(bchF)
MARUFIU81_16360
ROTFIV09_18150
RMHLVO79_15075(bchF)
MALUKU6B_16680(bchF)
RSPRSP_0284(bchF)
RSHRsph17029_1927
RSQRsph17025_1011
RSKRSKD131_1610
CAZTLV780_04400(bchF)
RCPRCAP_rcc00666(bchF)
RBLB6K69_03475
PTPRCA23_c29930(bchF)
RSUNHU_00210(bchF)
RHMB5V46_02450
RHCRGUI_1987
LVSLOKVESSMR4R_03603
RBGBG454_02925(bchF)
GEHHYN69_05420(bchF)
TAWEI545_18840(bchF)
POZI0K15_16890(bchF)
NSMJO391_16360(bchF)
FAQG5B39_09990(bchF)
HBCAEM38_14370
NOTC7W88_00555(bchF)
NGFFRF71_09220(bchF)
NOGGKE62_08510(bchF)
SPHITS85_23010
SSANNX02_18330
SCHYGVO57_11520(bchF)
SAERNEF64_07790(bchF)
CIJWG74_05110
BLASBSY18_2709(bchF)
BFWB5J99_04495
PALGHFP57_06875(bchF)
PARJJ4G78_07460(bchF)
SANDH3309_05815(bchF)
POQKZX46_18600(bchF)
PMEAKTC28_16910(bchF)
ALHG6N82_11510(bchF)
ELQGa0102493_111824
ERKCD351_13945(bchF)
ERFFIU90_13900
PNSA9D12_01355
PORLBG023_112684
PHZCHX26_14570(bchF)
POTE2E27_07265(bchF)
AMVACMV_18950(bchF)
SRFLHU95_13985(bchF)
RAPRHOA_2397
NCBC0V82_18050(bchF)
SKTIGS68_17000(bchF)
SMUCJL100_009045(bchF)
SROEJL101_015170(bchF)
RRURru_A0624
RRFF11_03200
RCERC1_2116(bchF)
RPMRSPPHO_00022(bchF)
ELIOKO353_02790(bchF)
RRSRoseRS_3262
RCARcas_3748
CAUCaur_0415
CHLChy400_0442
CAGCagg_3129
CTMCabther_B0080
CVLJ8C06_12190(bchF)
CHLOJ8C02_13075(bchF)
GPHGEMMAAP_06390
GGRHKW67_08900(bchF)
CTECT1421(bchF)
CPCCpar_0743
CLZBIU88_03610
CCHCag_0402
CPHCpha266_1790
CPBCphamn1_0939
CLIClim_0899
PVICvib_1239
PLTPlut_1421
PPHPpha_0981
PAAPaes_1533
PROCPtc2401_00669
PRSB9H02_04020
PROSCHL67_03610(bchF)
 » show all
Reference
1  [PMID:1080053]
  Authors
Pudek MR, Richards WR
  Title
A possible alternate pathway of bacteriochlorophyll biosynthesis in a mutant of Rhodopseudomonas sphaeroides.
  Journal
Biochemistry 14:3132-7 (1975)
DOI:10.1021/bi00685a015
Reference
2  [PMID:8385667]
  Authors
Burke DH, Alberti M, Hearst JE
  Title
bchFNBH bacteriochlorophyll synthesis genes of Rhodobacter capsulatus and identification of the third subunit of light-independent protochlorophyllide reductase in bacteria and plants.
  Journal
J Bacteriol 175:2414-22 (1993)
DOI:10.1128/JB.175.8.2414-2422.1993
  Sequence
Reference
3  [PMID:26088139]
  Authors
Lange C, Kiesel S, Peters S, Virus S, Scheer H, Jahn D, Moser J
  Title
Broadened Substrate Specificity of 3-Hydroxyethyl Bacteriochlorophyllide a Dehydrogenase (BchC) Indicates a New Route for the Biosynthesis of Bacteriochlorophyll a.
  Journal
J Biol Chem 290:19697-709 (2015)
DOI:10.1074/jbc.M115.660555
  Sequence
[cte:CT1421]
Reference
4  [PMID:26331578]
  Authors
Harada J, Teramura M, Mizoguchi T, Tsukatani Y, Yamamoto K, Tamiaki H
  Title
Stereochemical conversion of C3-vinyl group to 1-hydroxyethyl group in bacteriochlorophyll c by the hydratases BchF and BchV: adaptation of green sulfur bacteria to limited-light environments.
  Journal
Mol Microbiol 98:1184-98 (2015)
DOI:10.1111/mmi.13208
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.165
IUBMB Enzyme Nomenclature: 4.2.1.165
ExPASy - ENZYME nomenclature database: 4.2.1.165
BRENDA, the Enzyme Database: 4.2.1.165

KEGG   REACTION: R09059
Entry
R09059                      Reaction                               
Name
3-hydroxyethylchlorophyllide-a hydro-lyase (chlorophyllide-a-forming)
Definition
Chlorophyllide + H2O <=> 3-Hydroxyethylchlorophyllide a
Equation
Reaction class
RC02412  C02139_C18154
Enzyme
Pathway
rn00860  Porphyrin metabolism
rn01110  Biosynthesis of secondary metabolites
Orthology
K11336  3-vinyl bacteriochlorophyllide hydratase [EC:4.2.1.165]
Other DBs
RHEA: 15072

DBGET integrated database retrieval system