KEGG   ORTHOLOGY: K12467
Entry
K12467                      KO                                     

Name
E4.2.3.15
Definition
myrcene/ocimene synthase [EC:4.2.3.15]
Pathway
ko00902  Monoterpenoid biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00902 Monoterpenoid biosynthesis
    K12467  E4.2.3.15; myrcene/ocimene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.15  myrcene synthase
     K12467  E4.2.3.15; myrcene/ocimene synthase
Other DBs
RN: R02009
GO: 0050551
Genes
ATH: AT2G24210(TPS10) AT3G25810
CRB: 17889158 17889311
CSAT: 104702938
BRP: 103858386 103858582
BNA: 106385578 106402047 106443840
BOE: 106328108 106334082
RSZ: 108846403 108846672
AG: AAB71084(ag2)
Reference
PMID:9268308
  Authors
Bohlmann J, Steele CL, Croteau R
  Title
Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.
  Journal
J Biol Chem 272:21784-92 (1997)
DOI:10.1074/jbc.272.35.21784
  Sequence
Reference
  Authors
Chen F, Tholl D, D'Auria JC, Farooq A, Pichersky E, Gershenzon J
  Title
Biosynthesis and emission of terpenoid volatiles from Arabidopsis flowers.
  Journal
Plant Cell 15:481-94 (2003)
DOI:10.1105/tpc.007989

KEGG   ORTHOLOGY: K21925
Entry
K21925            Tight     KO                                     

Name
TPS3
Definition
(-)-camphene/tricyclene/(4S)-limonene/myrcene synthase [EC:4.2.3.117 4.2.3.105 4.2.3.16 4.2.3.15]
Pathway
ko00902  Monoterpenoid biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00902 Monoterpenoid biosynthesis
    K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.15  myrcene synthase
     K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
    4.2.3.16  (4S)-limonene synthase
     K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
    4.2.3.105  tricyclene synthase
     K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
    4.2.3.117  (-)-camphene synthase
     K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
Other DBs
RN: R02009 R02013 R09972
GO: 0102703 0102701 0050552 0050551
Genes
SLY: 101245212(TPS3)
SPEN: 107012939
SOT: 102591915
Reference
  Authors
Falara V, Akhtar TA, Nguyen TT, Spyropoulou EA, Bleeker PM, Schauvinhold I, Matsuba Y, Bonini ME, Schilmiller AL, Last RL, Schuurink RC, Pichersky E
  Title
The tomato terpene synthase gene family.
  Journal
Plant Physiol 157:770-89 (2011)
DOI:10.1104/pp.111.179648
  Sequence
[sly:101245212]

KEGG   ENZYME: 4.2.3.15
Entry
EC 4.2.3.15                 Enzyme                                 

Name
myrcene synthase
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
geranyl-diphosphate diphosphate-lyase (myrcene-forming)
Reaction(IUBMB)
geranyl diphosphate = myrcene + diphosphate [RN:R02009]
Reaction(KEGG)
R02009
Substrate
geranyl diphosphate [CPD:C00341]
Product
myrcene [CPD:C06074];
diphosphate [CPD:C00013]
Comment
A recombinant enzyme (also known as a monoterpene synthase or cyclase) from the grand fir (Abies grandis) requires Mn2+ and K+ for activity. Mg2+ is essentially ineffective as the divalent metal ion cofactor.
History
EC 4.2.3.15 created 2000 as EC 4.1.99.9, transferred 2000 to EC 4.2.3.15
Pathway
ec00902  Monoterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12467  myrcene/ocimene synthase
K21925  (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
Genes
ATH: AT2G24210(TPS10) AT3G25810
CRB: 17889158 17889311
CSAT: 104702938
BRP: 103858386 103858582
BNA: 106385578 106402047 106443840
BOE: 106328108 106334082
RSZ: 108846403 108846672
SLY: 101245212(TPS3)
SPEN: 107012939
SOT: 102591915
Reference
1  [PMID:9268308]
  Authors
Bohlmann J, Steele CL, Croteau R
  Title
Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.
  Journal
J Biol Chem 272:21784-92 (1997)
DOI:10.1074/jbc.272.35.21784
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.15
IUBMB Enzyme Nomenclature: 4.2.3.15
ExPASy - ENZYME nomenclature database: 4.2.3.15
BRENDA, the Enzyme Database: 4.2.3.15
CAS: 197462-59-2

KEGG   REACTION: R02009
Entry
R02009                      Reaction                               

Name
geranyldiphosphate diphosphate-lyase (myrcene-forming)
Definition
Geranyl diphosphate <=> Myrcene + Diphosphate
Equation
Comment
myrcene synthase
Reaction class
RC00637  C00341_C06074
Enzyme
Pathway
rn00902  Monoterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K12467  myrcene/ocimene synthase [EC:4.2.3.15]
K21925  (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase [EC:4.2.3.117 4.2.3.105 4.2.3.16 4.2.3.15]
Other DBs
RHEA: 16968

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