KEGG   ORTHOLOGY: K12676
Entry
K12676                      KO                                     
Symbol
pah
Name
proclavaminate amidinohydrolase [EC:3.5.3.22]
Pathway
map00331  Clavulanic acid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00674  Clavaminate biosynthesis, arginine + glyceraldehyde-3P => clavaminate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00331 Clavulanic acid biosynthesis
    K12676  pah; proclavaminate amidinohydrolase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.3  In linear amidines
    3.5.3.22  proclavaminate amidinohydrolase
     K12676  pah; proclavaminate amidinohydrolase
Other DBs
RN: R05357
GO: 0033972
Genes
APAU: AMPC_01500(speB_2)
RAP: RHOA_4422
SFA: Sfla_0557
STRP: F750_6322
SCLF: BB341_07815 BB341_29475
SFK: KY5_8016c
SKA: CP970_43085(speB)
SVR: CP971_33675(speB)
SSPB: CP982_00550(speB)
KAB: B7C62_18895
 » show all
Reference
PMID:8529893
  Authors
Hodgson JE, Fosberry AP, Rawlinson NS, Ross HN, Neal RJ, Arnell JC, Earl AJ, Lawlor EJ
  Title
Clavulanic acid biosynthesis in Streptomyces clavuligerus: gene cloning and characterization.
  Journal
Gene 166:49-55 (1995)
DOI:10.1016/0378-1119(95)00560-9
  Sequence
Reference
PMID:7601835
  Authors
Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA
  Title
Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.
  Journal
J Bacteriol 177:3714-20 (1995)
DOI:10.1128/JB.177.13.3714-3720.1995

KEGG   ENZYME: 3.5.3.22
Entry
EC 3.5.3.22                 Enzyme                                 
Name
proclavaminate amidinohydrolase;
PAH;
proclavaminate amidino hydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amidines
Sysname
amidinoproclavaminate amidinohydrolase
Reaction(IUBMB)
amidinoproclavaminate + H2O = proclavaminate + urea [RN:R05357]
Reaction(KEGG)
R05357
Substrate
amidinoproclavaminate [CPD:C06657];
H2O [CPD:C00001]
Product
proclavaminate [CPD:C06658];
urea [CPD:C00086]
Comment
Forms part of the pathway for the biosythesis of the beta-lactamase inhibitor clavulanate in Streptomyces clavuligerus. It carries out an intermediary reaction between the first reaction of EC 1.14.11.21, clavaminate synthase, and the second and third reactions of that enzyme. Requires Mn2+.
History
EC 3.5.3.22 created 2003
Pathway
ec00331  Clavulanic acid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12676  proclavaminate amidinohydrolase
Genes
APAUAMPC_01500(speB_2)
RAPRHOA_4422
SFASfla_0557
STRPF750_6322
SCLFBB341_07815 BB341_29475
SFKKY5_8016c
SKACP970_43085(speB)
SVRCP971_33675(speB)
SSPBCP982_00550(speB)
KABB7C62_18895
KAUB6264_26745
SVISvir_33400
 » show all
Reference
1  [PMID:1998687]
  Authors
Salowe SP, Krol WJ, Iwata-Reuyl D, Townsend CA.
  Title
Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction.
  Journal
Biochemistry 30:2281-92 (1991)
DOI:10.1021/bi00222a034
Reference
2  [PMID:11472170]
  Authors
Zhou J, Kelly WL, Bachmann BO, Gunsior M, Townsend CA, Solomon EI.
  Title
Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities.
  Journal
J Am Chem Soc 123:7388-98 (2001)
DOI:10.1021/ja004025+
Reference
3  [PMID:12413541]
  Authors
Townsend CA.
  Title
New reactions in clavulanic acid biosynthesis.
  Journal
Curr Opin Chem Biol 6:583-9 (2002)
DOI:10.1016/S1367-5931(02)00392-7
Reference
4  [PMID:7601835]
  Authors
Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA
  Title
Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.
  Journal
J Bacteriol 177:3714-20 (1995)
DOI:10.1128/JB.177.13.3714-3720.1995
Other DBs
ExplorEnz - The Enzyme Database: 3.5.3.22
IUBMB Enzyme Nomenclature: 3.5.3.22
ExPASy - ENZYME nomenclature database: 3.5.3.22
BRENDA, the Enzyme Database: 3.5.3.22

KEGG   REACTION: R05357
Entry
R05357                      Reaction                               
Name
amidinoproclavaminate amidinohydrolase
Definition
Guanidinoproclavaminic acid + H2O <=> Proclavaminic acid + Urea
Equation
Comment
pah gene
Reaction class
RC00024  C06657_C06658
RC00329  C00086_C06657
Enzyme
Pathway
rn00331  Clavulanic acid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00674  Clavaminate biosynthesis, arginine + glyceraldehyde-3P => clavaminate
Orthology
K12676  proclavaminate amidinohydrolase [EC:3.5.3.22]
Reference
1  [PMID:9614345]
  Authors
McGowan SJ, Bycroft BW, Salmond GP.
  Title
Bacterial production of carbapenems and clavams: evolution of beta-lactam antibiotic pathways.
  Journal
Trends Microbiol 6:203-8 (1998)
DOI:10.1016/S0966-842X(98)01251-7
Other DBs
RHEA: 17004

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