KEGG   ORTHOLOGY: K12697
Entry
K12697            Tight     KO                                     
Symbol
D4H
Name
deacetoxyvindoline 4-hydroxylase [EC:1.14.11.20]
Pathway
map00901  Indole alkaloid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00965  Vindoline biosynthesis, tabersonine => vindoline
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00901 Indole alkaloid biosynthesis
    K12697  D4H; deacetoxyvindoline 4-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.11  With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
    1.14.11.20  deacetoxyvindoline 4-hydroxylase
     K12697  D4H; deacetoxyvindoline 4-hydroxylase
Other DBs
RN: R05857
GO: 0050590
Genes
AG: AAB97311(D4H)
Reference
PMID:9290645
  Authors
Vazquez-Flota F, De Carolis E, Alarco AM, De Luca V
  Title
Molecular cloning and characterization of desacetoxyvindoline-4-hydroxylase, a 2-oxoglutarate dependent-dioxygenase involved in the biosynthesis of vindoline in Catharanthus roseus (L.) G. Don.
  Journal
Plant Mol Biol 34:935-48 (1997)
DOI:10.1023/A:1005894001516
  Sequence

KEGG   ENZYME: 1.14.11.20
Entry
EC 1.14.11.20               Enzyme                                 
Name
deacetoxyvindoline 4-hydroxylase;
desacetoxyvindoline 4-hydroxylase;
desacetyoxyvindoline-17-hydroxylase;
D17H;
desacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4beta-hydroxylating)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
deacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4beta-hydroxylating)
Reaction(IUBMB)
deacetoxyvindoline + 2-oxoglutarate + O2 = deacetylvindoline + succinate + CO2 [RN:R05857]
Reaction(KEGG)
R05857
Substrate
deacetoxyvindoline [CPD:C02673];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
deacetylvindoline [CPD:C01091];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. Also acts on 3-hydroxy-16-methoxy-2,3-dihydrotabersonine and to a lesser extent on 16-methoxy-2,3-dihydrotabersonine.
History
EC 1.14.11.20 created 2002, modified 2005
Pathway
ec00901  Indole alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12697  deacetoxyvindoline 4-hydroxylase
Reference
1  [PMID:16667836]
  Authors
De Carolis E, Chan F, Balsevich J, De Luca V
  Title
Isolation and Characterization of a 2-Oxoglutarate Dependent Dioxygenase Involved in the Second-to-Last Step in Vindoline Biosynthesis.
  Journal
Plant Physiol 94:1323-1329 (1990)
DOI:10.1104/pp.94.3.1323
  Sequence
Reference
2  [PMID:8449913]
  Authors
De Carolis E, De Luca V
  Title
Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus.
  Journal
J Biol Chem 268:5504-11 (1993)
  Sequence
Reference
3  [PMID:9701591]
  Authors
Vazquez-Flota FA, De Luca V.
  Title
Developmental and light regulation of desacetoxyvindoline 4-hydroxylase in catharanthus roseus (L.) G. Don. . Evidence Of a multilevel regulatory mechanism
  Journal
Plant Physiol 117:1351-61 (1998)
DOI:10.1104/pp.117.4.1351
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.20
IUBMB Enzyme Nomenclature: 1.14.11.20
ExPASy - ENZYME nomenclature database: 1.14.11.20
BRENDA, the Enzyme Database: 1.14.11.20
CAS: 132084-83-4

KEGG   REACTION: R05857
Entry
R05857                      Reaction                               
Name
desacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4b-hydroxylating)
Definition
Desacetoxyvindoline + 2-Oxoglutarate + Oxygen <=> Deacetylvindoline + Succinate + CO2
Equation
Reaction class
RC01480  C01091_C02673
Enzyme
Pathway
rn00901  Indole alkaloid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00965  Vindoline biosynthesis, tabersonine => vindoline
Orthology
K12697  deacetoxyvindoline 4-hydroxylase [EC:1.14.11.20]
Other DBs
RHEA: 18976

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