KEGG   ORTHOLOGY: K12708
Entry
K12708                      KO                                     
Symbol
novR, cloR
Name
4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase [EC:1.13.11.83 1.13.12.23]
Pathway
map00401  Novobiocin biosynthesis
map01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00401 Novobiocin biosynthesis
    K12708  novR, cloR; 4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.13  Acting on single donors with incorporation of molecular oxygen (oxygenases)
   1.13.11  With incorporation of two atoms of oxygen
    1.13.11.83  4-hydroxy-3-prenylphenylpyruvate oxygenase
     K12708  novR, cloR; 4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase
   1.13.12  With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
    1.13.12.23  4-hydroxy-3-prenylbenzoate synthase
     K12708  novR, cloR; 4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase
Other DBs
RN: R06758 R06759
COG: COG0235
Genes
NGV: CDO52_02235
AG: AAF67511(novR) AAN65240(cloR)
Reference
  Authors
Pojer F, Kahlich R, Kammerer B, Li SM, Heide L.
  Title
CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis.
  Journal
J Biol Chem 278:30661-8 (2003)
DOI:10.1074/jbc.M303190200
  Sequence

KEGG   ENZYME: 1.13.12.23
Entry
EC 1.13.12.23               Enzyme                                 
Name
4-hydroxy-3-prenylbenzoate synthase;
CloR;
novR (gene name)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
Sysname
4-hydroxy-3-prenylmandelate:oxygen oxidoreductase (4-hydroxy-3-prenylbenzoate forming)
Reaction(IUBMB)
4-hydroxy-3-prenylmandelate + O2 = 4-hydroxy-3-prenylbenzoate + CO2 + H2O [RN:R06759]
Reaction(KEGG)
R06759
Substrate
4-hydroxy-3-prenylmandelate [CPD:C12457];
O2 [CPD:C00007]
Product
4-hydroxy-3-prenylbenzoate [CPD:C12458];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
Isolated from the bacterium Streptomyces roseochromogenes DS 12976. A bifunctional enzyme involved in clorobiocin biosynthesis that also catalyses the activity of EC 1.13.11.83, 4-hydroxy-3-prenylphenylpyruvate oxygenase.
History
EC 1.13.12.23 created 2017
Pathway
ec00401  Novobiocin biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12708  4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase
Genes
NGVCDO52_02235
Reference
1  [PMID:12777382]
  Authors
Pojer F, Kahlich R, Kammerer B, Li SM, Heide L.
  Title
CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis.
  Journal
J Biol Chem 278:30661-8 (2003)
DOI:10.1074/jbc.M303190200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.13.12.23
IUBMB Enzyme Nomenclature: 1.13.12.23
ExPASy - ENZYME nomenclature database: 1.13.12.23
BRENDA, the Enzyme Database: 1.13.12.23

KEGG   REACTION: R06759
Entry
R06759                      Reaction                               
Name
4-hydroxy-3-prenylmandelate:oxygen oxidoreductase (4-hydroxy-3-prenylbenzoate forming)
Definition
3-Dimethylallyl-4-hydroxymandelic acid + Oxygen <=> 3-Dimethylallyl-4-hydroxybenzoate + CO2 + H2O
Equation
Reaction class
RC01679  C12457_C12458
Enzyme
Pathway
rn00401  Novobiocin biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K12708  4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase [EC:1.13.11.83 1.13.12.23]
Other DBs
RHEA: 52915

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