KEGG   ORTHOLOGY: K13307
Entry
K13307                      KO                                     

Name
tylMI
Definition
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase [EC:2.1.1.235]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00796  dTDP-D-mycaminose biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K13307  tylMI; dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.235  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase
     K13307  tylMI; dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase
Other DBs
RN: R06439
Genes
SAMB: SAM23877_5630(srm7)
MAU: Micau_3984
MIL: ML5_4437
AG: CAA57473(tylM1)
Reference
PMID:9031628
  Authors
Gandecha AR, Large SL, Cundliffe E
  Title
Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome.
  Journal
Gene 184:197-203 (1997)
DOI:10.1016/S0378-1119(96)00595-1
  Sequence

KEGG   ENZYME: 2.1.1.235
Entry
EC 2.1.1.235                Enzyme                                 

Name
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase;
TylM1
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose 3-N,N-dimethyltransferase
Reaction(IUBMB)
2 S-adenosyl-L-methionine + dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose = 2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-3,6-dideoxy-alpha-D-glucopyranose [RN:R06439]
Reaction(KEGG)
R06439
Substrate
S-adenosyl-L-methionine [CPD:C00019];
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose
Product
S-adenosyl-L-homocysteine [CPD:C00021];
dTDP-3-dimethylamino-3,6-dideoxy-alpha-D-glucopyranose
Comment
The enzyme is involved in the biosynthesis of mycaminose, an essential structural component of the macrolide antibiotic tylosin, which is produced by the bacterium Streptomyces fradiae.
History
EC 2.1.1.235 created 2011
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13307  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase
Genes
SAMB: SAM23877_5630(srm7)
MAU: Micau_3984
MIL: ML5_4437
Reference
1  [PMID:12119032]
  Authors
Chen H, Yamase H, Murakami K, Chang CW, Zhao L, Zhao Z, Liu HW
  Title
Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine.
  Journal
Biochemistry 41:9165-83 (2002)
DOI:10.1021/bi020245j
  Sequence
Reference
2  [PMID:21142177]
  Authors
Carney AE, Holden HM
  Title
Molecular architecture of TylM1 from Streptomyces fradiae: an N,N-dimethyltransferase involved in the production of dTDP-D-mycaminose.
  Journal
Biochemistry 50:780-7 (2011)
DOI:10.1021/bi101733y
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.235
IUBMB Enzyme Nomenclature: 2.1.1.235
ExPASy - ENZYME nomenclature database: 2.1.1.235
BRENDA, the Enzyme Database: 2.1.1.235

KEGG   REACTION: R06439
Entry
R06439                      Reaction                               

Name
S-adenosyl-L-methionine:dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose 3-N,N-dimethyltransferase
Definition
dTDP-3-amino-3,6-dideoxy-D-glucose + 2 S-Adenosyl-L-methionine <=> dTDP-D-mycaminose + 2 S-Adenosyl-L-homocysteine + 2 H+
Equation
C11925 + 2 C00019 <=> C11933 + 2 C00021 + 2 C00080
Comment
N,N-dimethyltransferase,TylM1
Reaction class
RC00003  C00019_C00021
RC01515  C11925_C11933
Enzyme
Pathway
rn00523  Polyketide sugar unit biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00796  dTDP-D-mycaminose biosynthesis
Orthology
K13307  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase [EC:2.1.1.235]
Other DBs
RHEA: 31674

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