KEGG   ORTHOLOGY: K13327
Entry
K13327                      KO                                     

Name
spnN, oleW
Definition
dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase [EC:1.1.1.384]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00801  dTDP-L-olivose biosynthesis
M00802  dTDP-D-forosamine biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K13327  spnN, oleW; dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.384  dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase
     K13327  spnN, oleW; dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase
Other DBs
RN: R05526
COG: COG0673
Genes
LEZ: GLE_2525
VCX: VAA049_3443
SDE: Sde_2126
GUR: Gura_4277
GEO: Geob_0429
DMA: DMR_07590
DCB: C3Y92_16660
DSA: Desal_3611
BGZ: XH91_11585
LYG: C1N55_04680
PPY: PPE_03773
PPM: PPSC2_19910(gfo1)
PPO: PPM_3988(gfo1)
PPOL: X809_36015
PPOY: RE92_17275
TPR: Tpau_3482
SBH: SBI_06875
SVE: SVEN_5995
SALS: SLNWT_0707
SFI: SFUL_5283
SALU: DC74_6605
SALL: SAZ_34340
STRE: GZL_01617
SLD: T261_6843
SAMB: SAM23877_5610(srm27)
SRW: TUE45_01311(gfo_2)
SLX: SLAV_40170(afr2)
SFK: KY5_6078
NDA: Ndas_4698
NAL: B005_1464
MMAR: MODMU_0768
AMYY: YIM_08350(afr1) YIM_11650(afr3) YIM_22845(gfo2)
PSEH: XF36_05040
PHH: AFB00_29745(selSVI)
AMI: Amir_4534
SESP: BN6_58940(sam33)
AHG: AHOG_15155(afr3) AHOG_15190(gfo1)
SAQ: Sare_1266
MIL: ML5_4431
MCAB: HXZ27_04095(evdS8)
OBG: Verru16b_02068(gfo_5)
RBA: RB1063
RUL: UC8_43180(afr_5)
BFR: BF0810
BCEL: BcellWH2_00544(gfo_1) BcellWH2_04966(gfo_2)
GFO: GFO_2034
PROC: Ptc2401_00808(afr)
NBV: T478_0168
AG: AAD55450(oleW) AAG23275(spnN) AAL14250(jadP)
 » show all
Reference
  Authors
Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW
  Title
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
  Journal
J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383
Reference
  Authors
Waldron C, Matsushima P, Rosteck PR Jr, Broughton MC, Turner J, Madduri K, Crawford KP, Merlo DJ, Baltz RH
  Title
Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa.
  Journal
Chem Biol 8:487-99 (2001)
DOI:10.1016/S1074-5521(01)00029-1
  Sequence
Reference
  Authors
Aguirrezabalaga I, Olano C, Allende N, Rodriguez L, Brana AF, Mendez C, Salas JA
  Title
Identification and expression of genes involved in biosynthesis of L-oleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus.
  Journal
Antimicrob Agents Chemother 44:1266-75 (2000)
DOI:10.1128/AAC.44.5.1266-1275.2000
  Sequence

KEGG   ENZYME: 1.1.1.384
Entry
EC 1.1.1.384                Enzyme                                 

Name
dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase;
KijD10;
dTDP-4-keto-2,6-dideoxy-D-glucose 3-oxidoreductase;
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose 3-oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose:NADP+ 3-oxidoreductase
Reaction(IUBMB)
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + NADP+ = dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + NADPH + H+ [RN:R05526]
Reaction(KEGG)
R05526
Substrate
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose [CPD:C11922];
NADP+ [CPD:C00006]
Product
dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose [CPD:C06620];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme is involved in the biosynthesis of several deoxysugars, including L-digitoxose, L- and D-olivose, L-oliose, D-mycarose and forosamine.
History
EC 1.1.1.384 created 2015
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13327  dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase
Genes
LEZ: GLE_2525
VCX: VAA049_3443
SDE: Sde_2126
GUR: Gura_4277
GEO: Geob_0429
DMA: DMR_07590
DCB: C3Y92_16660
DSA: Desal_3611
BGZ: XH91_11585
LYG: C1N55_04680
PPY: PPE_03773
PPM: PPSC2_19910(gfo1)
PPO: PPM_3988(gfo1)
PPOL: X809_36015
PPOY: RE92_17275
TPR: Tpau_3482
SBH: SBI_06875
SVE: SVEN_5995
SALS: SLNWT_0707
SFI: SFUL_5283
SALU: DC74_6605
SALL: SAZ_34340
STRE: GZL_01617
SLD: T261_6843
SAMB: SAM23877_5610(srm27)
SRW: TUE45_01311(gfo_2)
SLX: SLAV_40170(afr2)
SFK: KY5_6078
NDA: Ndas_4698
NAL: B005_1464
MMAR: MODMU_0768
AMYY: YIM_08350(afr1) YIM_11650(afr3) YIM_22845(gfo2)
PSEH: XF36_05040
PHH: AFB00_29745(selSVI)
AMI: Amir_4534
SESP: BN6_58940(sam33)
AHG: AHOG_15155(afr3) AHOG_15190(gfo1)
SAQ: Sare_1266
MIL: ML5_4431
MCAB: HXZ27_04095(evdS8)
OBG: Verru16b_02068(gfo_5)
RBA: RB1063
RUL: UC8_43180(afr_5)
BFR: BF0810
BCEL: BcellWH2_00544(gfo_1) BcellWH2_04966(gfo_2)
GFO: GFO_2034
PROC: Ptc2401_00808(afr)
NBV: T478_0168
 » show all
Reference
1  [PMID:10770761]
  Authors
Aguirrezabalaga I, Olano C, Allende N, Rodriguez L, Brana AF, Mendez C, Salas JA
  Title
Identification and expression of genes involved in biosynthesis of L-oleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus.
  Journal
Antimicrob Agents Chemother 44:1266-75 (2000)
DOI:10.1128/AAC.44.5.1266-1275.2000
  Sequence
Reference
2  [PMID:11932454]
  Authors
Wang L, White RL, Vining LC
  Title
Biosynthesis of the dideoxysugar component of jadomycin B: genes in the jad cluster of Streptomyces venezuelae ISP5230 for L-digitoxose assembly and transfer to the angucycline aglycone.
  Journal
Microbiology 148:1091-103 (2002)
DOI:10.1099/00221287-148-4-1091
  Sequence
Reference
3  [PMID:18345667]
  Authors
Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW
  Title
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
  Journal
J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383
Reference
4  [PMID:21598943]
  Authors
Kubiak RL, Holden HM
  Title
Combined structural and functional investigation of a C-3''-ketoreductase involved in the biosynthesis of dTDP-L-digitoxose.
  Journal
Biochemistry 50:5905-17 (2011)
DOI:10.1021/bi200514b
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.384
IUBMB Enzyme Nomenclature: 1.1.1.384
ExPASy - ENZYME nomenclature database: 1.1.1.384
BRENDA, the Enzyme Database: 1.1.1.384

KEGG   REACTION: R05526
Entry
R05526                      Reaction                               

Name
dTDP-4-oxo-2,6-dideoxy-D-glucose:NADP+ 3-oxidoreductase
Definition
dTDP-3,4-dioxo-2,6-dideoxy-D-glucose + NADPH + H+ <=> dTDP-4-oxo-2,6-dideoxy-D-glucose + NADP+
Equation
Reaction class
RC00001  C00005_C00006
RC00897  C06620_C11922
Enzyme
Pathway
rn00523  Polyketide sugar unit biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00801  dTDP-L-olivose biosynthesis
M00802  dTDP-D-forosamine biosynthesis
Orthology
K13327  dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase [EC:1.1.1.384]
Other DBs
RHEA: 44627

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