KEGG   ORTHOLOGY: K13329
Entry
K13329                      KO                                     

Name
spnR
Definition
dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase [EC:2.6.1.110]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00802  dTDP-D-forosamine biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K13329  spnR; dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01007 Amino acid related enzymes
    K13329  spnR; dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.6  Transferring nitrogenous groups
   2.6.1  Transaminases
    2.6.1.110  dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase
     K13329  spnR; dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase
Amino acid related enzymes [BR:ko01007]
 Aminotransferase (transaminase)
  Others
   K13329  spnR; dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase
Other DBs
RN: R08931
COG: COG0399
Genes
SAMB: SAM23877_5617(srm20)
SLX: SLAV_40480(arnB2)
SFK: KY5_6527c
AAB: A4R43_01825
AG: AAG23279(spnR)
Reference
  Authors
Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW
  Title
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
  Journal
J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383
Reference
  Authors
Waldron C, Matsushima P, Rosteck PR Jr, Broughton MC, Turner J, Madduri K, Crawford KP, Merlo DJ, Baltz RH
  Title
Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa.
  Journal
Chem Biol 8:487-99 (2001)
DOI:10.1016/S1074-5521(01)00029-1
  Sequence

KEGG   ENZYME: 2.6.1.110
Entry
EC 2.6.1.110                Enzyme                                 

Name
dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase;
SpnR;
TDP-4-keto-2,3,6-trideoxy-D-glucose 4-aminotransferase
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
Sysname
dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose:2-oxoglutarate aminotransferase
Reaction(IUBMB)
dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose + 2-oxoglutarate = dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + L-glutamate [RN:R08931]
Reaction(KEGG)
R08931
Substrate
dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose [CPD:C18033];
2-oxoglutarate [CPD:C00026]
Product
dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose [CPD:C18032];
L-glutamate [CPD:C00025]
Comment
A pyridoxal-phosphate protein. The enzyme, isolated from the bacterium Saccharopolyspora spinosa, participates in the biosynthesis of forosamine.
History
EC 2.6.1.110 created 2016
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13329  dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase
Genes
SAMB: SAM23877_5617(srm20)
SLX: SLAV_40480(arnB2)
SFK: KY5_6527c
AAB: A4R43_01825
Reference
1  [PMID:18345667]
  Authors
Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW
  Title
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
  Journal
J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.110
IUBMB Enzyme Nomenclature: 2.6.1.110
ExPASy - ENZYME nomenclature database: 2.6.1.110
BRENDA, the Enzyme Database: 2.6.1.110

KEGG   REACTION: R08931
Entry
R08931                      Reaction                               

Name
dTDP-4-amino-2,3,4,6-tetradeoxy-D-glucose:2-oxoglutarate aminotransferase
Definition
dTDP-4-oxo-2,3,6-trideoxy-D-glucose + L-Glutamate <=> dTDP-4-amino-2,3,4,6-tetradeoxy-D-glucose + 2-Oxoglutarate
Equation
Reaction class
RC00006  C00025_C00026
RC01514  C18032_C18033
Enzyme
Pathway
rn00523  Polyketide sugar unit biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00802  dTDP-D-forosamine biosynthesis
Orthology
K13329  dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase [EC:2.6.1.110]
Other DBs
RHEA: 49147

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