KEGG   ORTHOLOGY: K14036
Entry
K14036                      KO                                     
Symbol
KSL7, DTC1
Name
ent-cassa-12,15-diene synthase [EC:4.2.3.28]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K14036  KSL7, DTC1; ent-cassa-12,15-diene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.28  ent-cassa-12,15-diene synthase
     K14036  KSL7, DTC1; ent-cassa-12,15-diene synthase
Other DBs
RN: R09119
GO: 0034277
Genes
OSA: 4329724
DOSA: Os02t0570400-01(Os02g0570400)
Reference
  Authors
Cho EM, Okada A, Kenmoku H, Otomo K, Toyomasu T, Mitsuhashi W, Sassa T, Yajima A, Yabuta G, Mori K, Oikawa H, Toshima H, Shibuya N, Nojiri H, Omori T, Nishiyama M, Yamane H
  Title
Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor.
  Journal
Plant J 37:1-8 (2004)
DOI:10.1046/j.1365-313X.2003.01926.x
  Sequence
[osa:4329724]

KEGG   ENZYME: 4.2.3.28
Entry
EC 4.2.3.28                 Enzyme                                 
Name
ent-cassa-12,15-diene synthase;
OsDTC1;
OsKS7
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
ent-copalyl-diphosphate diphosphate-lyase (ent-cassa-12,15-diene-forming)
Reaction(IUBMB)
ent-copalyl diphosphate = ent-cassa-12,15-diene + diphosphate [RN:R09119]
Reaction(KEGG)
R09119
Substrate
ent-copalyl diphosphate [CPD:C06089]
Product
ent-cassa-12,15-diene [CPD:C18226];
diphosphate [CPD:C00013]
Comment
This class I diterpene cyclase produces ent-cassa-12,15-diene, a precursor of the rice phytoalexins (-)-phytocassanes A-E. Phytoalexins are diterpenoid secondary metabolites that are involved in the defense mechanism of the plant, and are produced in response to pathogen attack through the perception of elicitor signal molecules such as chitin oligosaccharide, or after exposure to UV irradiation.
History
EC 4.2.3.28 created 2008
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K14036  ent-cassa-12,15-diene synthase
Genes
OSA4329724
DOSAOs02t0570400-01(Os02g0570400)
Reference
1  [PMID:14675427]
  Authors
Cho EM, Okada A, Kenmoku H, Otomo K, Toyomasu T, Mitsuhashi W, Sassa T, Yajima A, Yabuta G, Mori K, Oikawa H, Toshima H, Shibuya N, Nojiri H, Omori T, Nishiyama M, Yamane H
  Title
Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor.
  Journal
Plant J 37:1-8 (2004)
DOI:10.1046/j.1365-313X.2003.01926.x
  Sequence
[osa:4329724]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.28
IUBMB Enzyme Nomenclature: 4.2.3.28
ExPASy - ENZYME nomenclature database: 4.2.3.28
BRENDA, the Enzyme Database: 4.2.3.28

KEGG   REACTION: R09119
Entry
R09119                      Reaction                               
Name
ent-Copalyl-diphosphate diphosphate-lyase (ent-cassa-12,15-diene-forming)
Definition
ent-Copalyl diphosphate <=> ent-Cassa-12,15-diene + Diphosphate
Equation
Reaction class
RC02437  C06089_C18226
Enzyme
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K14036  ent-cassa-12,15-diene synthase [EC:4.2.3.28]
Other DBs
RHEA: 25535

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