oxyL (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
4-hydroxy-6-methylpretetramide,NADPH:oxygen oxidoreductase (12a-hydroxylating)
4-hydroxy-6-methylpretetramide + NADPH + H+ + O2 = 4-de(dimethylamino)-4-oxoanhydrotetracycline + NADP+ + H2O [RN:
Contains FAD. The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. The enzyme is bifunctional, and can also catalyse EC
, 6-methylpretetramide 4-monooxygenase.
EC 188.8.131.52 created 2016
ec01057 Biosynthesis of type II polyketide products
ec01110 Biosynthesis of secondary metabolites
K14252 6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase
SPRI: SPRI_0201 SPRI_7152
SHUN: DWB77_05143(pcpB_4) » show all
Zhang W, Watanabe K, Cai X, Jung ME, Tang Y, Zhan J
Identifying the minimal enzymes required for anhydrotetracycline biosynthesis.