The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. It can also catalyse EC 184.108.40.206, 12-dehydrotetracycline 5-monooxygenase.
Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
R04060 Anhydrotetracycline + Oxygen + NADPH + H+ <=> 5a,11a-Dehydrotetracycline + NADP+ + H2O