KEGG   ORTHOLOGY: K15092
Entry
K15092            Tight     KO                                     

Name
E1.3.1.81
Definition
(+)-pulegone reductase [EC:1.3.1.81]
Pathway
ko00902  Monoterpenoid biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00902 Monoterpenoid biosynthesis
    K15092  E1.3.1.81; (+)-pulegone reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.1  With NAD+ or NADP+ as acceptor
    1.3.1.81  (+)-pulegone reductase
     K15092  E1.3.1.81; (+)-pulegone reductase
Other DBs
RN: R06419 R06420
GO: 0052579 0052580
Genes
AG: AAQ75423
Reference
  Authors
Ringer KL, McConkey ME, Davis EM, Rushing GW, Croteau R
  Title
Monoterpene double-bond reductases of the (-)-menthol biosynthetic pathway: isolation and characterization of cDNAs encoding (-)-isopiperitenone reductase and (+)-pulegone reductase of peppermint.
  Journal
Arch Biochem Biophys 418:80-92 (2003)
DOI:10.1016/S0003-9861(03)00390-4
  Sequence

KEGG   ENZYME: 1.3.1.81
Entry
EC 1.3.1.81                 Enzyme                                 

Name
(+)-pulegone reductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(-)-menthone:NADP+ oxidoreductase
Reaction(IUBMB)
(1) (-)-menthone + NADP+ = (+)-pulegone + NADPH + H+ [RN:R06419];
(2) (+)-isomenthone + NADP+ = (+)-pulegone + NADPH + H+ [RN:R06420]
Reaction(KEGG)
R06419 R06420
Substrate
(-)-menthone [CPD:C00843];
NADP+ [CPD:C00006];
(+)-isomenthone [CPD:C11952]
Product
(+)-pulegone [CPD:C09893];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
NADH cannot replace NADPH as reductant. The Delta8,9-double bond of (+)-cis-isopulegone and the Delta1,2-double bond of (+/-)-piperitone are not substrates. The enzyme from peppermint (Mentha x piperita) converts (+)-pulegone into both (-)-menthone and (+)-isomenthone at a ratio of 70:30 for native enzyme but it does not catalyse the reverse reaction. This enzyme is a member of the medium-chain dehydrogenase/reductase superfamily.
History
EC 1.3.1.81 created 2008
Pathway
ec00902  Monoterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K15092  (+)-pulegone reductase
Reference
1  [PMID:13679086]
  Authors
Ringer KL, McConkey ME, Davis EM, Rushing GW, Croteau R
  Title
Monoterpene double-bond reductases of the (-)-menthol biosynthetic pathway: isolation and characterization of cDNAs encoding (-)-isopiperitenone reductase and (+)-pulegone reductase of peppermint.
  Journal
Arch Biochem Biophys 418:80-92 (2003)
DOI:10.1016/S0003-9861(03)00390-4
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.81
IUBMB Enzyme Nomenclature: 1.3.1.81
ExPASy - ENZYME nomenclature database: 1.3.1.81
BRENDA, the Enzyme Database: 1.3.1.81

KEGG   REACTION: R06419
Entry
R06419                      Reaction                               

Name
(-)-menthone:NADP+ oxidoreductase
Definition
Pulegone + NADPH + H+ <=> (-)-Menthone + NADP+
Equation
Reaction class
RC00001  C00005_C00006
RC01510  C00843_C09893
Enzyme
Pathway
rn00902  Monoterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K15092  (+)-pulegone reductase [EC:1.3.1.81]
Other DBs
RHEA: 25644

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