KEGG   ORTHOLOGY: K15096
Entry
K15096                      KO                                     

Name
E4.2.3.20
Definition
(R)-limonene synthase [EC:4.2.3.20]
Pathway
ko00902  Monoterpenoid biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00902 Monoterpenoid biosynthesis
    K15096  E4.2.3.20; (R)-limonene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.20  (R)-limonene synthase
     K15096  E4.2.3.20; (R)-limonene synthase
Other DBs
RN: R06120
GO: 0034002
Genes
CIT: 102607029
AG: AAM53944
Reference
  Authors
Lucker J, El Tamer MK, Schwab W, Verstappen FW, van der Plas LH, Bouwmeester HJ, Verhoeven HA
  Title
Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases.
  Journal
Eur J Biochem 269:3160-71 (2002)
DOI:10.1046/j.1432-1033.2002.02985.x
  Sequence

KEGG   ENZYME: 4.2.3.20
Entry
EC 4.2.3.20                 Enzyme                                 

Name
(R)-limonene synthase;
(+)-limonene synthase;
geranyldiphosphate diphosphate lyase [(+)-(R)-limonene-forming];
geranyl-diphosphate diphosphate-lyase [cyclizing, (+)-(4R)-limonene-forming]
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
geranyl-diphosphate diphosphate-lyase [cyclizing, (R)-limonene-forming]
Reaction(IUBMB)
geranyl diphosphate = (R)-limonene + diphosphate [RN:R06120]
Reaction(KEGG)
R06120
Substrate
geranyl diphosphate [CPD:C00341]
Product
(R)-limonene [CPD:C06099];
diphosphate [CPD:C00013]
Comment
Forms the first step of carvone biosynthesis in caraway. The enzyme from Carum carvi (caraway) seeds requires a divalent metal ion (preferably Mn2+) for catalysis. This enzyme occurs in Citrus, Carum (caraway) and Anethum (dill); (-)-limonene, however, is made in the fir, Abies, and mint, Mentha, by EC 4.2.3.16, (4S)-limonene synthase.
History
EC 4.2.3.20 created 2003
Pathway
ec00902  Monoterpenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15096  (R)-limonene synthase
Genes
CIT: 102607029
Reference
1  [PMID:9662532]
  Authors
Bouwmeester HJ, Gershenzon J, Konings MC, Croteau R.
  Title
Biosynthesis of the monoterpenes limonene and carvone in the fruit of caraway. I. Demonstration Of enzyme activities and their changes with development
  Journal
Plant Physiol 117:901-12 (1998)
DOI:10.1104/pp.117.3.901
Reference
2  [PMID:12084056]
  Authors
Lucker J, El Tamer MK, Schwab W, Verstappen FW, van der Plas LH, Bouwmeester HJ, Verhoeven HA
  Title
Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases.
  Journal
Eur J Biochem 269:3160-71 (2002)
DOI:10.1046/j.1432-1033.2002.02985.x
  Sequence
Reference
3  [PMID:11305598]
  Authors
Maruyama T, Ito M, Kiuchi F, Honda G.
  Title
Molecular cloning, functional expression and characterization of d-limonene synthase from Schizonepeta tenuifolia.
  Journal
Biol Pharm Bull 24:373-7 (2001)
DOI:10.1248/bpb.24.373
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.20
IUBMB Enzyme Nomenclature: 4.2.3.20
ExPASy - ENZYME nomenclature database: 4.2.3.20
BRENDA, the Enzyme Database: 4.2.3.20
CAS: 155807-65-1

KEGG   REACTION: R06120
Entry
R06120                      Reaction                               

Name
geranyldiphosphate diphosphate lyase [(+)-(R)-limonene-forming]
Definition
Geranyl diphosphate <=> (R)-Limonene + Diphosphate
Equation
Reaction class
RC02771  C00341_C06099
Enzyme
Pathway
rn00902  Monoterpenoid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K15096  (R)-limonene synthase [EC:4.2.3.20]
Other DBs
RHEA: 10943

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