KEGG   ORTHOLOGY: K15944
Entry
K15944                      KO                                     
Symbol
snoaF, dnrE, dauE, aknU
Name
nogalaviketone/aklaviketone reductase [EC:1.1.1.- 1.1.1.362]
Pathway
map01057  Biosynthesis of type II polyketide products
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00781  Nogalavinone/aklavinone biosynthesis, deoxynogalonate/deoxyaklanonate => nogalavinone/aklavinone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.362  aklaviketone reductase
     K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
    1.1.1.-
     K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Others
   K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
Other DBs
RN: R06677 R06682 R09329
Genes
SPHW: NFX46_24955
SGAL: CP966_29005
SCOE: CP976_32935
SXN: IAG42_36850
SDD: D9753_08660
TALX: FOF52_07460
MAU: Micau_4000
MIL: ML5_4421
DMAT: Dmats_05325
AG: AAA65202(dnrE) AAB08021(dauE) AAF01806(snoaF) AAF73457(aknU)
Reference
  Authors
Torkkell S, Kunnari T, Palmu K, Mantsala P, Hakala J, Ylihonko K
  Title
The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures.
  Journal
Mol Genet Genomics 266:276-88 (2001)
DOI:10.1007/s004380100554
  Sequence
Reference
PMID:8655529
  Authors
Dickens ML, Ye J, Strohl WR
  Title
Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5.
  Journal
J Bacteriol 178:3384-8 (1996)
DOI:10.1128/JB.178.11.3384-3388.1996
  Sequence
Reference
  Authors
Raty K, Kunnari T, Hakala J, Mantsala P, Ylihonko K
  Title
A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin.
  Journal
Mol Gen Genet 264:164-72 (2000)
DOI:10.1007/s004380000306
  Sequence

KEGG   ENZYME: 1.1.1.362
Entry
EC 1.1.1.362                Enzyme                                 
Name
aklaviketone reductase;
dauE (gene name);
aknU (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
aklavinone:NADP+ oxidoreductase
Reaction(IUBMB)
aklavinone + NADP+ = aklaviketone + NADPH + H+ [RN:R06682]
Reaction(KEGG)
R06682;
(other) R06677
Substrate
aklavinone [CPD:C12424];
NADP+ [CPD:C00006]
Product
aklaviketone [CPD:C12423];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme is involved in the synthesis of the aklavinone aglycone, a common precursor for several anthracycline antibiotics including aclacinomycins, daunorubicin and doxorubicin. The enzyme from the Gram-negative bacterium Streptomyces sp. C5 produces daunomycin.
History
EC 1.1.1.362 created 2013
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15944  nogalaviketone/aklaviketone reductase
Genes
SPHWNFX46_24955
SGALCP966_29005
SCOECP976_32935
SXNIAG42_36850
SDDD9753_08660
TALXFOF52_07460
MAUMicau_4000
MILML5_4421
DMATDmats_05325
Reference
1  [PMID:8655529]
  Authors
Dickens ML, Ye J, Strohl WR
  Title
Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5.
  Journal
J Bacteriol 178:3384-8 (1996)
DOI:10.1128/JB.178.11.3384-3388.1996
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.362
IUBMB Enzyme Nomenclature: 1.1.1.362
ExPASy - ENZYME nomenclature database: 1.1.1.362
BRENDA, the Enzyme Database: 1.1.1.362

KEGG   REACTION: R06677
Entry
R06677                      Reaction                               
Name
auramycinone:NADP+ 7-oxidoreductase
Definition
Auraviketone + NADPH + H+ <=> Auramycinone + NADP+
Equation
Reaction class
RC00001  C00005_C00006
RC00234  C12417_C12419
Enzyme
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01110  Biosynthesis of secondary metabolites
Orthology
K15944  nogalaviketone/aklaviketone reductase [EC:1.1.1.- 1.1.1.362]
Reference
1  [PMID:10658662]
  Authors
Kantola J, Kunnari T, Hautala A, Hakala J, Ylihonko K, Mantsala P
  Title
Elucidation of anthracyclinone biosynthesis by stepwise cloning of genes for anthracyclines from three different Streptomyces spp.
  Journal
Microbiology 146 ( Pt 1):155-63 (2000)
DOI:10.1099/00221287-146-1-155

DBGET integrated database retrieval system