KEGG   ORTHOLOGY: K17070
Entry
K17070                      KO                                     

Symbol
dcmA
Name
dichloromethane dehalogenase [EC:4.5.1.3]
Pathway
map00625  Chloroalkane and chloroalkene degradation
map01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00625 Chloroalkane and chloroalkene degradation
    K17070  dcmA; dichloromethane dehalogenase
Enzymes [BR:ko01000]
 4. Lyases
  4.5  Carbon-halide lyases
   4.5.1  Carbon-halide lyases (only sub-subclass identified to date)
    4.5.1.3  dichloromethane dehalogenase
     K17070  dcmA; dichloromethane dehalogenase
Other DBs
RN: R00603
GO: 0018834
Genes
MDI: METDI2656(dcmA)
HDN: Hden_0711
Reference
  Authors
Vuilleumier S, Chistoserdova L, Lee MC, Bringel F, Lajus A, Zhou Y, Gourion B, Barbe V, Chang J, Cruveiller S, Dossat C, Gillett W, Gruffaz C, Haugen E, Hourcade E, Levy R, Mangenot S, Muller E, Nadalig T, Pagni M, Penny C, Peyraud R, Robinson DG, Roche D, Rouy Z, Saenampechek C, Salvignol G, Vallenet D, Wu Z, Marx CJ, Vorholt JA, Olson MV, Kaul R, Weissenbach J, Medigue C, Lidstrom ME
  Title
Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources.
  Journal
PLoS One 4:e5584 (2009)
DOI:10.1371/journal.pone.0005584
  Sequence
[mdi:METDI2656]

KEGG   ENZYME: 4.5.1.3
Entry
EC 4.5.1.3                  Enzyme                                 

Name
dichloromethane dehalogenase;
dichloromethane chloride-lyase (chloride-hydrolysing)
Class
Lyases;
Carbon-halide lyases;
Carbon-halide lyases (only sub-subclass identified to date)
Sysname
dichloromethane chloride-lyase (adding H2O; chloride-hydrolysing; formaldehyde-forming)
Reaction(IUBMB)
dichloromethane + H2O = formaldehyde + 2 chloride [RN:R00603]
Reaction(KEGG)
R00603
Substrate
dichloromethane [CPD:C02271];
H2O [CPD:C00001]
Product
formaldehyde [CPD:C00067];
chloride [CPD:C00698]
Comment
Requires glutathione.
History
EC 4.5.1.3 created 1989
Pathway
ec00625  Chloroalkane and chloroalkene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K17070  dichloromethane dehalogenase
Genes
MDI: METDI2656(dcmA)
HDN: Hden_0711
Reference
1  [PMID:3988708]
  Authors
Kohler-Staub D, Leisinger T.
  Title
Dichloromethane dehalogenase of Hyphomicrobium sp. strain DM2.
  Journal
J Bacteriol 162:676-81 (1985)
DOI:10.1128/JB.162.2.676-681.1985
Other DBs
ExplorEnz - The Enzyme Database: 4.5.1.3
IUBMB Enzyme Nomenclature: 4.5.1.3
ExPASy - ENZYME nomenclature database: 4.5.1.3
UM-BBD (Biocatalysis/Biodegradation Database): 4.5.1.3
BRENDA, the Enzyme Database: 4.5.1.3
CAS: 97002-70-5

KEGG   REACTION: R00603
Entry
R00603                      Reaction                               

Name
dichloromethane chloride-lyase (adding H2O; chloride-hydrolysing; formaldehyde-forming)
Definition
Dichloromethane + H2O <=> Formaldehyde + 2 Hydrochloric acid
Equation
Reaction class
RC00337  C00067_C02271
Enzyme
Pathway
rn00625  Chloroalkane and chloroalkene degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K17070  dichloromethane dehalogenase [EC:4.5.1.3]
Other DBs
RHEA: 15400

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