KEGG   ORTHOLOGY: K17476
Entry
K17476                      KO                                     
Symbol
penM, pntM, CYP161C
Name
pentalenolactone synthase [EC:1.14.19.8]
Pathway
map00998  Biosynthesis of various antibiotics
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00819  Pentalenolactone biosynthesis, farnesyl-PP => pentalenolactone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various antibiotics
    K17476  penM, pntM, CYP161C; pentalenolactone synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K17476  penM, pntM, CYP161C; pentalenolactone synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.8  pentalenolactone synthase
     K17476  penM, pntM, CYP161C; pentalenolactone synthase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, bacteria type
  CYP161 family
   K17476  penM, pntM, CYP161C; pentalenolactone synthase
Other DBs
RN: R10162
COG: COG2124
Genes
SBH: SBI_09672(cyp47)
SCW: TU94_27370
SLE: sle_13230(sle_13230)
SSIA: A7J05_09275
SGJ: IAG43_27120
SHK: J2N69_35840
AG: ADO85571(pntM) ADO85587(penM)
Reference
PMID:21284395 (CYP161C2 CYP161C3)
  Authors
Zhu D, Seo MJ, Ikeda H, Cane DE
  Title
Genome mining in streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone.
  Journal
J Am Chem Soc 133:2128-31 (2011)
DOI:10.1021/ja111279h
  Sequence

KEGG   ENZYME: 1.14.19.8
Entry
EC 1.14.19.8                Enzyme                                 
Name
pentalenolactone synthase;
penM (gene name);
pntM (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
pentalenolactone-reduced-ferredoxin:oxygen oxidoreductase (pentalenolactone-forming)
Reaction(IUBMB)
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O [RN:R10162]
Reaction(KEGG)
R10162
Substrate
pentalenolactone F [CPD:C20397];
O2 [CPD:C00007];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]
Product
pentalenolactone [CPD:C20407];
oxidized ferredoxin [CPD:C00139];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). Isolated from the bacteria Streptomyces exfoliatus and Streptomyces arenae.
History
EC 1.14.19.8 created 2012 as EC 1.3.7.10, transferred 2013 to EC 1.14.19.8
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K17476  pentalenolactone synthase
Genes
SBHSBI_09672(cyp47)
SCWTU94_27370
SLEsle_13230(sle_13230)
SSIAA7J05_09275
SGJIAG43_27120
SHKJ2N69_35840
Reference
1  [PMID:21284395]
  Authors
Zhu D, Seo MJ, Ikeda H, Cane DE
  Title
Genome mining in streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone.
  Journal
J Am Chem Soc 133:2128-31 (2011)
DOI:10.1021/ja111279h
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.8
IUBMB Enzyme Nomenclature: 1.14.19.8
ExPASy - ENZYME nomenclature database: 1.14.19.8
BRENDA, the Enzyme Database: 1.14.19.8

KEGG   REACTION: R10162
Entry
R10162                      Reaction                               
Name
pentalenolactone-reduced-ferredoxin:oxygen oxidoreductase (pentalenolactone forming)
Definition
Pentalenolactone F + Oxygen + 2 Reduced ferredoxin + 2 H+ <=> Pentalenolactone + 2 Oxidized ferredoxin + 2 H2O
Equation
C20397 + C00007 + 2 C00138 + 2 C00080 <=> C20407 + 2 C00139 + 2 C00001
Reaction class
RC03132  C20397_C20407
Enzyme
Pathway
rn00998  Biosynthesis of various antibiotics
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00819  Pentalenolactone biosynthesis, farnesyl-PP => pentalenolactone
Orthology
K17476  pentalenolactone synthase [EC:1.14.19.8]
Other DBs
RHEA: 34578

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