KEGG   ORTHOLOGY: K19854
Entry
K19854                      KO                                     

Name
tylB
Definition
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose transaminase [EC:2.6.1.89]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00796  dTDP-D-mycaminose biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K19854  tylB; dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose transaminase
Enzymes [BR:ko01000]
 2. Transferases
  2.6  Transferring nitrogenous groups
   2.6.1  Transaminases
    2.6.1.89  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose transaminase
     K19854  tylB; dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose transaminase
Other DBs
RN: R06423
Genes
SAMB: SAM23877_5622(srm15)
AG: AAA21342(tylB)
Reference
  Authors
Melancon CE 3rd, Hong L, White JA, Liu YN, Liu HW
  Title
Characterization of TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from the D-mycaminose biosynthetic pathway of Streptomyces fradiae: in vitro activity and substrate specificity studies.
  Journal
Biochemistry 46:577-90 (2007)
DOI:10.1021/bi061907y
Reference
  Authors
Karray F, Darbon E, Oestreicher N, Dominguez H, Tuphile K, Gagnat J, Blondelet-Rouault MH, Gerbaud C, Pernodet JL
  Title
Organization of the biosynthetic gene cluster for the macrolide antibiotic spiramycin in Streptomyces ambofaciens.
  Journal
Microbiology 153:4111-22 (2007)
DOI:10.1099/mic.0.2007/009746-0
  Sequence
Reference
PMID:7984112
  Authors
Merson-Davies LA, Cundliffe E
  Title
Analysis of five tylosin biosynthetic genes from the tyllBA region of the Streptomyces fradiae genome.
  Journal
Mol Microbiol 13:349-55 (1994)
DOI:10.1111/j.1365-2958.1994.tb00428.x
  Sequence

KEGG   ENZYME: 2.6.1.89
Entry
EC 2.6.1.89                 Enzyme                                 

Name
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose transaminase;
TylB;
TDP-3-keto-6-deoxy-D-glucose 3-aminotransferase;
TDP-3-dehydro-6-deoxy-D-glucose 3-aminotransferase;
dTDP-3-keto-6-deoxy-D-glucose 3-aminotransferase;
dTDP-3-dehydro-6-deoxy-D-glucose 3-aminotransferase
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
Sysname
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose:2-oxoglutarate aminotransferase
Reaction(IUBMB)
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose + 2-oxoglutarate = dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose + L-glutamate [RN:R06423]
Reaction(KEGG)
R06423
Substrate
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose;
2-oxoglutarate [CPD:C00026]
Product
dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose [CPD:C11908];
L-glutamate [CPD:C00025]
Comment
A pyridoxal-phosphate protein. The reaction occurs in the reverse direction. The enzyme is involved in biosynthesis of D-mycaminose.
History
EC 2.6.1.89 created 2011
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec00541  O-Antigen nucleotide sugar biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K19854  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose transaminase
K24250  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose transaminase
Genes
SAMB: SAM23877_5622(srm15)
Reference
1  [PMID:17209568]
  Authors
Melancon CE 3rd, Hong L, White JA, Liu YN, Liu HW
  Title
Characterization of TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from the D-mycaminose biosynthetic pathway of Streptomyces fradiae: in vitro activity and substrate specificity studies.
  Journal
Biochemistry 46:577-90 (2007)
DOI:10.1021/bi061907y
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.89
IUBMB Enzyme Nomenclature: 2.6.1.89
ExPASy - ENZYME nomenclature database: 2.6.1.89
BRENDA, the Enzyme Database: 2.6.1.89

KEGG   REACTION: R06423
Entry
R06423                      Reaction                               

Name
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose:2-oxoglutarate aminotransferase
Definition
3,6-Dideoxy-3-oxo-dTDP-D-glucose + L-Glutamate <=> dTDP-3-amino-3,6-dideoxy-D-glucose + 2-Oxoglutarate
Equation
Reaction class
RC00006  C00025_C00026
RC00781  C11908_C11925
Enzyme
Pathway
rn00523  Polyketide sugar unit biosynthesis
rn00541  O-Antigen nucleotide sugar biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00796  dTDP-D-mycaminose biosynthesis
Orthology
K19854  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose transaminase [EC:2.6.1.89]
K24250  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose transaminase [EC:2.6.1.89]
Other DBs
RHEA: 31670

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