KEGG   ORTHOLOGY: K19963
Entry
K19963                      KO                                     
Symbol
nylC
Name
6-aminohexanoate-oligomer endohydrolase [EC:3.5.1.117]
Pathway
map00930  Caprolactam degradation
map01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00930 Caprolactam degradation
    K19963  nylC; 6-aminohexanoate-oligomer endohydrolase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.1  In linear amides
    3.5.1.117  6-aminohexanoate-oligomer endohydrolase
     K19963  nylC; 6-aminohexanoate-oligomer endohydrolase
Other DBs
RN: R10978 R10979
Genes
MICR: BMW26_04430
MRN: K8F61_06370
MICS: C1N74_01735
ASOI: MTP13_02070 MTP13_02075 MTP13_02450
LTR: EVS81_05135
AGG: C1N71_00300
GSD: M3M28_12475
ACTW: F7P10_35555
NOA: BKM31_31370
NGN: LCN96_13830
AMYY: YIM_16760
AG: BAA05088(nylC)
 » show all
Reference
  Authors
Negoro S
  Title
Biodegradation of nylon oligomers.
  Journal
Appl Microbiol Biotechnol 54:461-6 (2000)
DOI:10.1007/s002530000434
  Sequence
Reference
PMID:1459943
  Authors
Negoro S, Kakudo S, Urabe I, Okada H
  Title
A new nylon oligomer degradation gene (nylC) on plasmid pOAD2 from a Flavobacterium sp.
  Journal
J Bacteriol 174:7948-53 (1992)
DOI:10.1128/JB.174.24.7948-7953.1992
  Sequence

KEGG   ENZYME: 3.5.1.117
Entry
EC 3.5.1.117                Enzyme                                 
Name
6-aminohexanoate-oligomer endohydrolase;
endo-type 6-aminohexanoate oligomer hydrolase;
Ahx endo-type-oligomer hydrolase;
6-aminohexanoate oligomer hydrolase;
Ahx-oligomer hydrolase;
nylon hydrolase;
nylon-oligomer hydrolase;
NylC;
nylon-6 hydrolase (ambiguous)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
6-aminohexanoate oligomer endoamidohydrolase
Reaction(IUBMB)
[N-(6-aminohexanoyl)]n + H2O = [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x [RN:R10976]
Reaction(KEGG)
R10976;
(other) R10978 R10979
Substrate
[N-(6-aminohexanoyl)]n [CPD:C20988];
H2O [CPD:C00001]
Product
[N-(6-aminohexanoyl)]n-x;
[N-(6-aminohexanoyl)]x
Comment
The enzyme is involved in degradation of nylon-6 oligomers. It degrades linear or cyclic oligomers of poly(6-aminohexanoate) with a degree of polymerization greater than three (n > 3) by endo-type cleavage, to oligomers of a length of two or more (2 <= x < n). It shows negligible activity with N-(6-aminohexanoyl)-6-aminohexanoate (cf. EC 3.5.1.46, 6-aminohexanoate-oligomer exo hydrolase) or with 1,8-diazacyclotetradecane-2,9-dione (cf. EC 3.5.2.12, 6-aminohexanoate-cyclic-dimer hydrolase).
History
EC 3.5.1.117 created 2014
Pathway
ec00930  Caprolactam degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K19963  6-aminohexanoate-oligomer endohydrolase
Genes
MICRBMW26_04430
MRNK8F61_06370
MICSC1N74_01735
ASOIMTP13_02070 MTP13_02075 MTP13_02450
LTREVS81_05135
AGGC1N71_00300
GSDM3M28_12475
ACTWF7P10_35555
NOABKM31_31370
NGNLCN96_13830
AMYYYIM_16760
PMADBAY61_11955
MICHFJK98_13105
MCABHXZ27_01495
 » show all
Reference
1  [PMID:8285701]
  Authors
Kakudo S, Negoro S, Urabe I, Okada H
  Title
Nylon oligomer degradation gene, nylC, on plasmid pOAD2 from a Flavobacterium strain encodes endo-type 6-aminohexanoate oligomer hydrolase: purification and characterization of the nylC gene product.
  Journal
Appl Environ Microbiol 59:3978-80 (1993)
DOI:10.1128/AEM.59.11.3978-3980.1993
Reference
2  [PMID:17827307]
  Authors
Yasuhira K, Tanaka Y, Shibata H, Kawashima Y, Ohara A, Kato D, Takeo M, Negoro S
  Title
6-Aminohexanoate oligomer hydrolases from the alkalophilic bacteria Agromyces sp. strain KY5R and Kocuria sp. strain KY2.
  Journal
Appl Environ Microbiol 73:7099-102 (2007)
DOI:10.1128/AEM.00777-07
Reference
3  [PMID:22187439]
  Authors
Negoro S, Shibata N, Tanaka Y, Yasuhira K, Shibata H, Hashimoto H, Lee YH, Oshima S, Santa R, Oshima S, Mochiji K, Goto Y, Ikegami T, Nagai K, Kato D, Takeo M, Higuchi Y
  Title
Three-dimensional structure of nylon hydrolase and mechanism of nylon-6 hydrolysis.
  Journal
J Biol Chem 287:5079-90 (2012)
DOI:10.1074/jbc.M111.321992
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.117
IUBMB Enzyme Nomenclature: 3.5.1.117
ExPASy - ENZYME nomenclature database: 3.5.1.117
BRENDA, the Enzyme Database: 3.5.1.117

KEGG   REACTION: R10979
Entry
R10979                      Reaction                               
Definition
[N-(6-Aminohexanoyl)]n <=> N-(6-Aminohexanoyl)-6-aminohexanoate
Equation
Comment
exo-type cleavage (ec 3.5.1.46)
endo-type cleavage (ec 3.5.1.117)
Enzyme
3.5.1.46        3.5.1.117
Pathway
rn00930  Caprolactam degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K01453  6-aminohexanoate-oligomer exohydrolase [EC:3.5.1.46]
K19963  6-aminohexanoate-oligomer endohydrolase [EC:3.5.1.117]

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