KEGG   ORTHOLOGY: K20616
Entry
K20616                      KO                                     
Symbol
lyeJ
Name
lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming) [EC:2.5.1.150]
Pathway
map00906  Carotenoid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00906 Carotenoid biosynthesis
    K20616  lyeJ; lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming)
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.150  lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming)
     K20616  lyeJ; lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming)
Other DBs
RN: R12285 R12286
COG: COG0382
Genes
MMAB: HQ865_22155
SABU: MBM09_03340
MTH: MTH_1809
MMG: MTBMA_c03790
MWO: MWSIV6_0342(lye)
METC: MTCT_1649
METE: tca_01747(menA_2)
METK: FVF72_02655
MTHM: FZP57_01590
MEME: HYG87_08515
HAL: VNG_1682C
HSL: OE_3380R(lye)
HDL: HALDL1_13225(ubiA)
HHB: Hhub_3181(lye)
HJE: HacjB3_05885(ubiA)
HALH: HTSR_0321(ubiA)
HHSR: HSR6_0307(ubiA)
HSU: HLASF_0239(ubiA)
HSF: HLASA_0239(ubiA)
SALI: L593_04760(ubiA)
HARA: AArcS_2863(ubiA2)
HMA: rrnAC0320(ubiA)
HHI: HAH_1057(ubiA1)
HHN: HISP_05430(ubiA)
HAB: SG26_13100(ubiA)
NPH: NP_4766A(lyeJ)
NMO: Nmlp_3027(lye)
HUT: Huta_2374
HTI: HTIA_2107
HMU: Hmuk_1361
HALL: LC1Hm_0582(ubiA)
HDS: HSR122_2629(ubiA)
HWA: HQ_2862A(lyeJ)
HWC: Hqrw_3256(lyeJ)
HVO: HVO_2527(lye)
HME: HFX_2549(ubiA)
HGI: ABY42_12665(ubiA)
HLA: Hlac_1898
HDF: AArcSl_1204(lyeJ)
HTU: Htur_2703
NMG: Nmag_1002(lye)
NAT: NJ7G_1611
HLR: HALLA_16020(ubiA)
POG: Pogu_1956
AG: BAP82510(lyeJ)
 » show all
Reference
  Authors
Yang Y, Yatsunami R, Ando A, Miyoko N, Fukui T, Takaichi S, Nakamura S
  Title
Complete biosynthetic pathway of the C50 carotenoid bacterioruberin from lycopene in the extremely halophilic archaeon Haloarcula japonica.
  Journal
J Bacteriol 197:1614-23 (2015)
DOI:10.1128/JB.02523-14
  Sequence

KEGG   ENZYME: 2.5.1.150
Entry
EC 2.5.1.150                Enzyme                                 
Name
lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming);
lbtA (gene name);
lyeJ (gene name);
dimethylallyl-diphosphate:all-trans-lycopene dimethylallyltransferase (hydrating, dihydrobisanhydrobacterioruberin-forming)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
prenyl-diphosphate:all-trans-lycopene prenyltransferase (hydrating, dihydrobisanhydrobacterioruberin-forming)
Reaction(IUBMB)
(1) prenyl diphosphate + all-trans-lycopene + H2O = dihydroisopentenyldehydrorhodopin + diphosphate [RN:R12285];
(2) prenyl diphosphate + isopentenyldehydrorhodopin + H2O = dihydrobisanhydrobacterioruberin + diphosphate [RN:R12286]
Reaction(KEGG)
R12285 R12286
Substrate
prenyl diphosphate [CPD:C00235];
all-trans-lycopene [CPD:C05432];
H2O [CPD:C00001];
isopentenyldehydrorhodopin [CPD:C22059]
Product
dihydroisopentenyldehydrorhodopin [CPD:C22058];
diphosphate [CPD:C00013];
dihydrobisanhydrobacterioruberin [CPD:C22060]
Comment
The enzyme, characterized from the bacterium Dietzia sp. CQ4 and the halophilic archaea Halobacterium salinarum and Haloarcula japonica, is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2 as well as the hydroxylation of the previous end of the molecule. The enzyme acts at both ends of the substrate, and combined with the action of EC 1.3.99.37, 1-hydroxy-2-isopentenylcarotenoid 3,4-desaturase, it forms the C50 carotenoid dihydrobisanhydrobacterioruberin. cf. EC 2.5.1.149, lycopene elongase/hydratase (flavuxanthin-forming).
History
EC 2.5.1.150 created 2018
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20616  lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming)
Genes
MMABHQ865_22155
SABUMBM09_03340
MTHMTH_1809
MMGMTBMA_c03790
MWOMWSIV6_0342(lye)
METCMTCT_1649
METEtca_01747(menA_2)
METKFVF72_02655
MTHMFZP57_01590
MEMEHYG87_08515
MZHMzhil_0946
MEMMemar_0118
HALVNG_1682C
HSLOE_3380R(lye)
HDLHALDL1_13225(ubiA)
HHBHhub_3181(lye)
HAGSJT689_08125
HABOJRZ79_06540
HNOLT974_11790
HLULT972_03120
HANRLJ422_06795
HJEHacjB3_05885(ubiA)
HALHHTSR_0321(ubiA)
HHSRHSR6_0307(ubiA)
HSUHLASF_0239(ubiA)
HSFHLASA_0239(ubiA)
SALRFQU85_06790
HALREFA46_012295
HAADMW046_14465
HALXM0R89_12785
HAXZM0R88_12940
SALIL593_04760(ubiA)
SAIMK0C01_06235
HARAAArcS_2863(ubiA2)
HMArrnAC0320(ubiA)
HHIHAH_1057(ubiA1)
HHNHISP_05430(ubiA)
HABSG26_13100(ubiA)
HTABVU17_05175
HALJG9465_16510
HSINKDQ40_08205
NPHNP_4766A(lyeJ)
NMONmlp_3027(lye)
NHOHWV23_16740
NSALHWV07_18230
HUTHuta_2374
HTIHTIA_2107
HALAHrd1104_01370
HMUHmuk_1361
HALZE5139_09965
HALLLC1Hm_0582(ubiA)
HAZPGBQ70_09960
HALIBV210_13220
HSNDV733_06790
HDSHSR122_2629(ubiA)
HRRHZS55_20280
HPELHZS54_23765
HLTI7X12_14755
HARCHARCEL1_04565
HDOMUK72_08395
HWAHQ_2862A(lyeJ)
HWCHqrw_3256(lyeJ)
HVOHVO_2527(lye)
HMEHFX_2549(ubiA)
HGIABY42_12665(ubiA)
HALEG3A49_16060
HLSKU306_00325
HBOHbor_10660
HAQDU484_15075
HAJDU500_15105
HAERDU502_13075
HRAEI982_16640
HLMDV707_06360
HALMFCF25_03145
HLAHlac_1898
HALPDOS48_09170
HALBEKH57_01635
HEZZEO776_09985
HALQHrr1229_010280
HSSJ7656_06540
HSAIHPS36_12060
HAZZKI388_07985
SRUBC2R22_15235
HAEhalTADL_0468
HALNB4589_011165
HALGHUG10_00790
HALUHUG12_16790
HMPK6T50_14390
HREK6T36_14340
HRMK6T25_02465
HACBHbl1158_06185
HDFAArcSl_1204(lyeJ)
HAHHalar_2116
HTUHtur_2703
HDABB347_07030 BB347_09720
HSALJMJ58_15355
HLOJ0X27_13915
HAKZJ0X25_02310
NMGNmag_1002(lye)
HXAHalxa_2221
NATNJ7G_1611
NPENatpe_1080
NVRFEJ81_05635
NPLFGF80_05290
NAYHYG81_05195
HALYHYG82_06625
HJTDVR14_14700
NGENatgr_2201
HRUHalru_1198
NOUNatoc_1918
HLRHALLA_16020(ubiA)
HLCCHINAEXTREME08690
NAJB1756_14585
NAGAArcMg_2864
NANAArc1_0839
NBGDV706_10180
NASGCU68_01895
SAWLNGM29_09965
POGPogu_1956
PYWPYWP30_01287
CSUCSUB_C0584
 » show all
Reference
1  [PMID:17008032]
  Authors
Tao L, Yao H, Cheng Q
  Title
Genes from a Dietzia sp. for synthesis of C40 and C50 beta-cyclic carotenoids.
  Journal
Gene 386:90-7 (2007)
DOI:10.1016/j.gene.2006.08.006
  Sequence
Reference
2  [PMID:21840984]
  Authors
Dummer AM, Bonsall JC, Cihla JB, Lawry SM, Johnson GC, Peck RF
  Title
Bacterioopsin-mediated regulation of bacterioruberin biosynthesis in Halobacterium salinarum.
  Journal
J Bacteriol 193:5658-67 (2011)
DOI:10.1128/JB.05376-11
  Sequence
[hal:VNG_1682C]
Reference
3  [PMID:25712483]
  Authors
Yang Y, Yatsunami R, Ando A, Miyoko N, Fukui T, Takaichi S, Nakamura S
  Title
Complete biosynthetic pathway of the C50 carotenoid bacterioruberin from lycopene in the extremely halophilic archaeon Haloarcula japonica.
  Journal
J Bacteriol 197:1614-23 (2015)
DOI:10.1128/JB.02523-14
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.150
IUBMB Enzyme Nomenclature: 2.5.1.150
ExPASy - ENZYME nomenclature database: 2.5.1.150
BRENDA, the Enzyme Database: 2.5.1.150

KEGG   REACTION: R12286
Entry
R12286                      Reaction                               
Name
dimethylallyl-diphosphate:isopentenyldehydrorhodopin dimethylallyltransferase (hydrating, dihydrobisanhydrobacterioruberin-forming)
Definition
Dimethylallyl diphosphate + Isopentenyldehydrorhodopin + H2O <=> Dihydrobisanhydrobacterioruberin + Diphosphate
Equation
Reaction class
RC03572  C22059_C22060
Enzyme
Pathway
rn00906  Carotenoid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K20616  lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming) [EC:2.5.1.150]

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