KEGG   ORTHOLOGY: K21202
Entry
K21202            Tight     KO                                     
Symbol
2-ODD
Name
(-)-deoxypodophyllotoxin synthase [EC:1.14.20.8]
Pathway
map00999  Biosynthesis of various plant secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various plant secondary metabolites
    K21202  2-ODD; (-)-deoxypodophyllotoxin synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.20  With 2-oxoglutarate as one donor, and the other dehydrogenated
    1.14.20.8  (-)-deoxypodophyllotoxin synthase
     K21202  2-ODD; (-)-deoxypodophyllotoxin synthase
Other DBs
RN: R11210
Genes
AG: ALG05135(2-ODD)
Reference
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence

KEGG   ENZYME: 1.14.20.8
Entry
EC 1.14.20.8                Enzyme                                 
Name
(-)-deoxypodophyllotoxin synthase;
2-ODD (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated
Sysname
(-)-yatein,2-oxoglutarate:oxygen oxidoreductase (ring-forming)
Reaction(IUBMB)
(-)-yatein + 2-oxoglutarate + O2 = (-)-deoxypodophyllotoxin + succinate + CO2 + H2O [RN:R11210]
Reaction(KEGG)
R11210
Substrate
(-)-yatein [CPD:C10557];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(-)-deoxypodophyllotoxin [CPD:C10556];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the plant Sinopodophyllum hexandrum (mayapple), is involved in the biosynthetic pathway of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs. It catalyses the closure of the central six-membered ring in the aryltetralin scaffold.
History
EC 1.14.20.8 created 2016 as EC 1.14.11.50, transferred 2018 to EC 1.14.20.8
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21202  (-)-deoxypodophyllotoxin synthase
Reference
1  [PMID:26359402]
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.20.8
IUBMB Enzyme Nomenclature: 1.14.20.8
ExPASy - ENZYME nomenclature database: 1.14.20.8
BRENDA, the Enzyme Database: 1.14.20.8

KEGG   REACTION: R11210
Entry
R11210                      Reaction                               
Name
(-)-yatein,2-oxoglutarate:oxygen oxidoreductase (ring-forming)
Definition
Dihydroanhydropodorhizol + 2-Oxoglutarate + Oxygen <=> Deoxypodophyllotoxin + Succinate + CO2 + H2O
Equation
Reaction class
RC03387  C10556_C10557
Enzyme
Pathway
rn00999  Biosynthesis of various plant secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00902  Podophyllotoxin biosynthesis, coniferyl alcohol => podophyllotoxin
Orthology
K21202  (-)-deoxypodophyllotoxin synthase [EC:1.14.20.8]
Other DBs
RHEA: 48747

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