KEGG   ORTHOLOGY: K21354
Entry
K21354            Tight     KO                                     
Symbol
UGT94E5
Name
beta-D-glucosyl crocetin beta-1,6-glucosyltransferase [EC:2.4.1.330]
Pathway
map00999  Biosynthesis of various plant secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00900  Crocin biosynthesis, crocetin => crocin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various plant secondary metabolites
    K21354  UGT94E5; beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.330  beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
     K21354  UGT94E5; beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
Other DBs
RN: R10988 R10989 R10990
CAZy: GT1
Genes
AG: BAK55744(UGT94E5)
Reference
  Authors
Nagatoshi M, Terasaka K, Owaki M, Sota M, Inukai T, Nagatsu A, Mizukami H
  Title
UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides.
  Journal
FEBS Lett 586:1055-61 (2012)
DOI:10.1016/j.febslet.2012.03.003
  Sequence

KEGG   ENZYME: 2.4.1.330
Entry
EC 2.4.1.330                Enzyme                                 
Name
beta-D-glucosyl crocetin beta-1,6-glucosyltransferase;
UGT94E5;
UDP-glucose:crocetin glucosyl ester glucosyltransferasee
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-alpha-D-glucose:beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
Reaction(IUBMB)
(1) UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + beta-D-gentiobiosyl crocetin [RN:R10988];
(2) UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin [RN:R10989];
(3) UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin = UDP + crocin [RN:R10990]
Reaction(KEGG)
Substrate
UDP-alpha-D-glucose [CPD:C00029];
beta-D-glucosyl crocetin [CPD:C19867];
bis(beta-D-glucosyl) crocetin [CPD:C19868];
beta-D-gentiobiosyl beta-D-glucosyl crocetin [CPD:C17513]
Product
UDP [CPD:C00015];
beta-D-gentiobiosyl crocetin [CPD:C19869];
beta-D-gentiobiosyl beta-D-glucosyl crocetin [CPD:C17513];
crocin [CPD:C08589]
Comment
The enzyme, characterized from the plant Gardenia jasminoides, adds a glucose to several crocetin glycosyl esters, but not to crocetin (cf. EC 2.4.1.271, crocetin glucosyltransferase).
History
EC 2.4.1.330 created 2014
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21354  beta-D-glucosyl crocetin beta-1,6-glucosyltransferase
Reference
1  [PMID:22569263]
  Authors
Nagatoshi M, Terasaka K, Owaki M, Sota M, Inukai T, Nagatsu A, Mizukami H
  Title
UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides.
  Journal
FEBS Lett 586:1055-61 (2012)
DOI:10.1016/j.febslet.2012.03.003
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.330
IUBMB Enzyme Nomenclature: 2.4.1.330
ExPASy - ENZYME nomenclature database: 2.4.1.330
BRENDA, the Enzyme Database: 2.4.1.330

KEGG   REACTION: R10990
Entry
R10990                      Reaction                               
Name
UDP-alpha-D-glucose:beta-D-gentiobiosyl-beta-D-glucosyl-crocetin beta-1,6-glucosyltransferase
Definition
UDP-glucose + trans-Crocetin (beta-D-glucosyl) (beta-D-gentibiosyl) ester <=> UDP + Crocin
Equation
Reaction class
RC00005  C00015_C00029
RC00059  C08589_C17513
Enzyme
Pathway
rn00999  Biosynthesis of various plant secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00900  Crocin biosynthesis, crocetin => crocin
Orthology
K21354  beta-D-glucosyl crocetin beta-1,6-glucosyltransferase [EC:2.4.1.330]
Other DBs
RHEA: 38862

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